Desmin cytoskeletal modifications after a bout of eccentric exercise in the rat
1 Departments of Orthopaedics and Bioengineering, and the Biomedical Sciences Graduate Group, University of California and Veterans Affairs Medical Centers, San Diego, California 92161; and 2 Department of Hand Surgery, Sahlgrenska University Hospital, SE405 30 Göteborg, Sweden Desmin content and...
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| Veröffentlicht in: | American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 2002-10, Vol.283 (4), p.958-R963 |
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| container_end_page | R963 |
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| container_issue | 4 |
| container_start_page | 958 |
| container_title | American journal of physiology. Regulatory, integrative and comparative physiology |
| container_volume | 283 |
| creator | Barash, Ilona A Peters, David Friden, Jan Lutz, Gordon J Lieber, Richard L |
| description | 1 Departments of Orthopaedics and Bioengineering,
and the Biomedical Sciences Graduate Group, University of California
and Veterans Affairs Medical Centers, San Diego, California 92161;
and 2 Department of Hand Surgery, Sahlgrenska
University Hospital, SE405 30 Göteborg,
Sweden
Desmin
content and immunohistochemical appearance were measured in tibialis
anterior muscles of rats subjected to a single bout of 30 eccentric
contractions (ECs). Ankle torque was measured before EC and at various
recovery times, after which immunohistochemical and immunoblot analyses
were performed. Torque decreased by ~50% immediately after EC and
fully recovered 168 h later ( P |
| doi_str_mv | 10.1152/ajpregu.00185.2002 |
| format | Article |
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and the Biomedical Sciences Graduate Group, University of California
and Veterans Affairs Medical Centers, San Diego, California 92161;
and 2 Department of Hand Surgery, Sahlgrenska
University Hospital, SE405 30 Göteborg,
Sweden
Desmin
content and immunohistochemical appearance were measured in tibialis
anterior muscles of rats subjected to a single bout of 30 eccentric
contractions (ECs). Ankle torque was measured before EC and at various
recovery times, after which immunohistochemical and immunoblot analyses
were performed. Torque decreased by ~50% immediately after EC and
fully recovered 168 h later ( P < 0.001). Loss of
desmin staining was maximal 12 h after EC and recovered by 72 h. Immunoblots unexpectedly demonstrated a significant increase in the
desmin-to-actin ratio by 72 h after EC ( P < 0.01)
and was still increasing after 168 h ( P < 0.0001). These data demonstrate a relatively rapid qualitative loss of
desmin immunostaining immediately after a single EC bout but a
tremendous quantitative increase in desmin content 72-168 h later.
This dynamic restructuring of the muscle's intermediate filament
system may be involved in the mechanism of EC-induced muscle injury and
may provide a structural explanation for the protective effects
observed in muscle after a single EC bout.
intermediate filaments; muscle injury; calpain; cytoskeleton; biomechanics</description><identifier>ISSN: 0363-6119</identifier><identifier>EISSN: 1522-1490</identifier><identifier>DOI: 10.1152/ajpregu.00185.2002</identifier><identifier>PMID: 12228066</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Cytoskeleton - metabolism ; Desmin - metabolism ; Immunoblotting ; Immunohistochemistry ; Male ; Motor Activity - physiology ; Muscle, Skeletal - metabolism ; Rats ; Rats, Sprague-Dawley ; Time Factors</subject><ispartof>American journal of physiology. Regulatory, integrative and comparative physiology, 2002-10, Vol.283 (4), p.958-R963</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-3fc1d3cb073ad8f3d4f2c6c9ecb0c42b115bb434b8e876da46fb89fb9c1a01e33</citedby><cites>FETCH-LOGICAL-c453t-3fc1d3cb073ad8f3d4f2c6c9ecb0c42b115bb434b8e876da46fb89fb9c1a01e33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,3040,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12228066$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barash, Ilona A</creatorcontrib><creatorcontrib>Peters, David</creatorcontrib><creatorcontrib>Friden, Jan</creatorcontrib><creatorcontrib>Lutz, Gordon J</creatorcontrib><creatorcontrib>Lieber, Richard L</creatorcontrib><title>Desmin cytoskeletal modifications after a bout of eccentric exercise in the rat</title><title>American journal of physiology. Regulatory, integrative and comparative physiology</title><addtitle>Am J Physiol Regul Integr Comp Physiol</addtitle><description>1 Departments of Orthopaedics and Bioengineering,
and the Biomedical Sciences Graduate Group, University of California
and Veterans Affairs Medical Centers, San Diego, California 92161;
and 2 Department of Hand Surgery, Sahlgrenska
University Hospital, SE405 30 Göteborg,
Sweden
Desmin
content and immunohistochemical appearance were measured in tibialis
anterior muscles of rats subjected to a single bout of 30 eccentric
contractions (ECs). Ankle torque was measured before EC and at various
recovery times, after which immunohistochemical and immunoblot analyses
were performed. Torque decreased by ~50% immediately after EC and
fully recovered 168 h later ( P < 0.001). Loss of
desmin staining was maximal 12 h after EC and recovered by 72 h. Immunoblots unexpectedly demonstrated a significant increase in the
desmin-to-actin ratio by 72 h after EC ( P < 0.01)
and was still increasing after 168 h ( P < 0.0001). These data demonstrate a relatively rapid qualitative loss of
desmin immunostaining immediately after a single EC bout but a
tremendous quantitative increase in desmin content 72-168 h later.
This dynamic restructuring of the muscle's intermediate filament
system may be involved in the mechanism of EC-induced muscle injury and
may provide a structural explanation for the protective effects
observed in muscle after a single EC bout.
intermediate filaments; muscle injury; calpain; cytoskeleton; biomechanics</description><subject>Animals</subject><subject>Cytoskeleton - metabolism</subject><subject>Desmin - metabolism</subject><subject>Immunoblotting</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Motor Activity - physiology</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Time Factors</subject><issn>0363-6119</issn><issn>1522-1490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1KAzEURoMoWqsv4EKycjc1P9NpZilqVRAKUtchydy00ZlmTDLYvr1T2-LK1YXc73zcHISuKBlROma36qMNsOhGhFAxHjFC2BEa9AuW0bwkx2hAeMGzgtLyDJ3H-EEIyXnOT9EZZYwJUhQDNHuA2LgVNpvk4yfUkFSNG18564xKzq8iVjZBwApr3yXsLQZjYJWCMxjWEIyLgPuCtAQcVLpAJ1bVES73c4jep4_z--fsdfb0cn_3mpl8zFPGraEVN5pMuKqE5VVumSlMCf2TyZnu_6d1f6wWICZFpfLCalFaXRqqCAXOh-hm19sG_9VBTLJx0UBdqxX4LsoJI2JCy22Q7YIm-BgDWNkG16iwkZTIrUa51yh_Ncqtxh663rd3uoHqD9l76wPlLrB0i-W3CyDb5SY6X_vFRk67up7DOh2ameAyl2_lWMi2sj2b_c8ejvlj-A8bHJaF</recordid><startdate>20021001</startdate><enddate>20021001</enddate><creator>Barash, Ilona A</creator><creator>Peters, David</creator><creator>Friden, Jan</creator><creator>Lutz, Gordon J</creator><creator>Lieber, Richard L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20021001</creationdate><title>Desmin cytoskeletal modifications after a bout of eccentric exercise in the rat</title><author>Barash, Ilona A ; Peters, David ; Friden, Jan ; Lutz, Gordon J ; Lieber, Richard L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-3fc1d3cb073ad8f3d4f2c6c9ecb0c42b115bb434b8e876da46fb89fb9c1a01e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Cytoskeleton - metabolism</topic><topic>Desmin - metabolism</topic><topic>Immunoblotting</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Motor Activity - physiology</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barash, Ilona A</creatorcontrib><creatorcontrib>Peters, David</creatorcontrib><creatorcontrib>Friden, Jan</creatorcontrib><creatorcontrib>Lutz, Gordon J</creatorcontrib><creatorcontrib>Lieber, Richard L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barash, Ilona A</au><au>Peters, David</au><au>Friden, Jan</au><au>Lutz, Gordon J</au><au>Lieber, Richard L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Desmin cytoskeletal modifications after a bout of eccentric exercise in the rat</atitle><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle><addtitle>Am J Physiol Regul Integr Comp Physiol</addtitle><date>2002-10-01</date><risdate>2002</risdate><volume>283</volume><issue>4</issue><spage>958</spage><epage>R963</epage><pages>958-R963</pages><issn>0363-6119</issn><eissn>1522-1490</eissn><abstract>1 Departments of Orthopaedics and Bioengineering,
and the Biomedical Sciences Graduate Group, University of California
and Veterans Affairs Medical Centers, San Diego, California 92161;
and 2 Department of Hand Surgery, Sahlgrenska
University Hospital, SE405 30 Göteborg,
Sweden
Desmin
content and immunohistochemical appearance were measured in tibialis
anterior muscles of rats subjected to a single bout of 30 eccentric
contractions (ECs). Ankle torque was measured before EC and at various
recovery times, after which immunohistochemical and immunoblot analyses
were performed. Torque decreased by ~50% immediately after EC and
fully recovered 168 h later ( P < 0.001). Loss of
desmin staining was maximal 12 h after EC and recovered by 72 h. Immunoblots unexpectedly demonstrated a significant increase in the
desmin-to-actin ratio by 72 h after EC ( P < 0.01)
and was still increasing after 168 h ( P < 0.0001). These data demonstrate a relatively rapid qualitative loss of
desmin immunostaining immediately after a single EC bout but a
tremendous quantitative increase in desmin content 72-168 h later.
This dynamic restructuring of the muscle's intermediate filament
system may be involved in the mechanism of EC-induced muscle injury and
may provide a structural explanation for the protective effects
observed in muscle after a single EC bout.
intermediate filaments; muscle injury; calpain; cytoskeleton; biomechanics</abstract><cop>United States</cop><pmid>12228066</pmid><doi>10.1152/ajpregu.00185.2002</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0363-6119 |
| ispartof | American journal of physiology. Regulatory, integrative and comparative physiology, 2002-10, Vol.283 (4), p.958-R963 |
| issn | 0363-6119 1522-1490 |
| language | eng |
| recordid | cdi_crossref_primary_10_1152_ajpregu_00185_2002 |
| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Cytoskeleton - metabolism Desmin - metabolism Immunoblotting Immunohistochemistry Male Motor Activity - physiology Muscle, Skeletal - metabolism Rats Rats, Sprague-Dawley Time Factors |
| title | Desmin cytoskeletal modifications after a bout of eccentric exercise in the rat |
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