Review—CRISPR/Cas Systems: Endless Possibilities for Electrochemical Nucleic Acid Sensors
The CRISPR/Cas system has gained enormous attention for its excellent gene-editing capabilities. In recent years, the reported trans-cleavage activity of some Cas proteins, including Cas12, Cas13 and Cas14, has given the CRISPR/Cas system an increasingly powerful molecular diagnostic ability. When t...
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Veröffentlicht in: | Journal of the Electrochemical Society 2022-03, Vol.169 (3), p.37522 |
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container_title | Journal of the Electrochemical Society |
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creator | Zhuang, Xiwei Yang, Xueqiong Cao, Bo Sun, Haiming Lv, Xiaoyan Zeng, Chijia Li, Fugang Qu, Bin Zhou, H. Susan Cui, Feiyun Zhou, Qin |
description | The CRISPR/Cas system has gained enormous attention for its excellent gene-editing capabilities. In recent years, the reported trans-cleavage activity of some Cas proteins, including Cas12, Cas13 and Cas14, has given the CRISPR/Cas system an increasingly powerful molecular diagnostic ability. When the CRISPR/Cas system is introduced into the field of electrochemical (EC) biosensor, it confers the high specificity to distinguish single base mismatches of nucleic acid, excellent sensitivity with the limit of detection as low as attomole range, and well meets the point-of-care testing (POCT) requirements of nucleic acid testing (NAT). In this review, we have briefly introduced the history and inherent advantages of the CRISPR/Cas system. The EC sensing platforms based on CRISPR/Cas systems have been compared with the classical fluorescence and colorimetric platforms. And the isothermal amplification strategies suitable for CRISPR/Cas system have been summarized. After that, we have highlighted the application of EC biosensor based on CRISPR/Cas system (EC-CRISPR) in the detection and identification of cancers, bacteria and viruses. Finally, the future prospects of EC-CRISPR have been proposed. |
doi_str_mv | 10.1149/1945-7111/ac5cec |
format | Article |
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The EC sensing platforms based on CRISPR/Cas systems have been compared with the classical fluorescence and colorimetric platforms. And the isothermal amplification strategies suitable for CRISPR/Cas system have been summarized. After that, we have highlighted the application of EC biosensor based on CRISPR/Cas system (EC-CRISPR) in the detection and identification of cancers, bacteria and viruses. Finally, the future prospects of EC-CRISPR have been proposed.</description><identifier>ISSN: 0013-4651</identifier><identifier>EISSN: 1945-7111</identifier><identifier>DOI: 10.1149/1945-7111/ac5cec</identifier><identifier>CODEN: JESOAN</identifier><language>eng</language><publisher>IOP Publishing</publisher><subject>CRISPR/Cas system ; electrochemical (EC) biosensor ; nucleic acid testing (NAT) ; point-of-care testing (POCT)</subject><ispartof>Journal of the Electrochemical Society, 2022-03, Vol.169 (3), p.37522</ispartof><rights>2022 The Electrochemical Society (“ECS”). 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In this review, we have briefly introduced the history and inherent advantages of the CRISPR/Cas system. The EC sensing platforms based on CRISPR/Cas systems have been compared with the classical fluorescence and colorimetric platforms. And the isothermal amplification strategies suitable for CRISPR/Cas system have been summarized. After that, we have highlighted the application of EC biosensor based on CRISPR/Cas system (EC-CRISPR) in the detection and identification of cancers, bacteria and viruses. 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Soc</addtitle><date>2022-03-01</date><risdate>2022</risdate><volume>169</volume><issue>3</issue><spage>37522</spage><pages>37522-</pages><issn>0013-4651</issn><eissn>1945-7111</eissn><coden>JESOAN</coden><abstract>The CRISPR/Cas system has gained enormous attention for its excellent gene-editing capabilities. In recent years, the reported trans-cleavage activity of some Cas proteins, including Cas12, Cas13 and Cas14, has given the CRISPR/Cas system an increasingly powerful molecular diagnostic ability. When the CRISPR/Cas system is introduced into the field of electrochemical (EC) biosensor, it confers the high specificity to distinguish single base mismatches of nucleic acid, excellent sensitivity with the limit of detection as low as attomole range, and well meets the point-of-care testing (POCT) requirements of nucleic acid testing (NAT). In this review, we have briefly introduced the history and inherent advantages of the CRISPR/Cas system. The EC sensing platforms based on CRISPR/Cas systems have been compared with the classical fluorescence and colorimetric platforms. And the isothermal amplification strategies suitable for CRISPR/Cas system have been summarized. After that, we have highlighted the application of EC biosensor based on CRISPR/Cas system (EC-CRISPR) in the detection and identification of cancers, bacteria and viruses. Finally, the future prospects of EC-CRISPR have been proposed.</abstract><pub>IOP Publishing</pub><doi>10.1149/1945-7111/ac5cec</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-6659-6965</orcidid><orcidid>https://orcid.org/0000-0002-6834-354X</orcidid><orcidid>https://orcid.org/0000-0001-5055-1423</orcidid></addata></record> |
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source | Institute of Physics Journals |
subjects | CRISPR/Cas system electrochemical (EC) biosensor nucleic acid testing (NAT) point-of-care testing (POCT) |
title | Review—CRISPR/Cas Systems: Endless Possibilities for Electrochemical Nucleic Acid Sensors |
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