Oxidation of the mercurous ion by peroxidase

Oxidation of the mercurous ion by peroxidase

The kinetics of oxidation of the mercurous ion by peroxidase have been measured by following the disappearance of mercurous ion using cold-vapour atomic absorption spectroscopy. Pseudo-first-order kinetics are observed with respect to mercurous ion, and the pseudo-first-order rate constants are line...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Canadian journal of chemistry 1986-05, Vol.64 (5), p.969-972
Hauptverfasser: Wigfield, Donald C, Tse, Season
Format: Artikel
Sprache:eng
Schlagworte:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Oxidoreductases
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 972
container_issue 5
container_start_page 969
container_title Canadian journal of chemistry
container_volume 64
creator Wigfield, Donald C
Tse, Season
description The kinetics of oxidation of the mercurous ion by peroxidase have been measured by following the disappearance of mercurous ion using cold-vapour atomic absorption spectroscopy. Pseudo-first-order kinetics are observed with respect to mercurous ion, and the pseudo-first-order rate constants are linearly related to peroxidase concentration, showing first-order dependence on peroxidase. This behaviour is identical to oxidation of elemental mercury, and the second-order rate constant, 1.44 × 10 4  M −1 s −1 at 23 °C, is also, within experimental error, the same as that for elemental mercury oxidation. The data are interpreted in terms of peroxidase-induced disproportionation of the mercurous dimer, followed by two-electron oxidation of zero-valent mercury.
doi_str_mv 10.1139/v86-162
format Article
fullrecord <record><control><sourceid>pascalfrancis_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1139_v86_162</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>8188223</sourcerecordid><originalsourceid>FETCH-LOGICAL-c282t-db408fe9f2be103746cec17d1fae5299979aae1dc40a327882512f0dfb820f263</originalsourceid><addsrcrecordid>eNp1z01Lw0AQBuBFFIxV_As5iIIYnZl87R6l-AWFXvQcNptZGmmTsJuK_ffd0tKD4GmY4eEdXiGuER4RU_X0I4sECzoREWYSkpQUnooIAGSSQUbn4sL777CWQHkkHua_baPHtu_i3sbjguMVO7N2_drHu2O9iQd2_Q55vhRnVi89Xx3mRHy9vnxO35PZ_O1j-jxLDEkak6bOQFpWlmpGSMusMGywbNBqzkkpVSqtGRuTgU6plJJyJAuNrSWBpSKdiLt9rnG9945tNbh2pd2mQqh2JatQsgolg7zZy0F7o5fW6c60_sglhnBKA7vds84Zx561M4sjOmRVQ2MDvP8f_n2-BZsna8g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Oxidation of the mercurous ion by peroxidase</title><source>NRC Research Press</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Free Full-Text Journals in Chemistry</source><creator>Wigfield, Donald C ; Tse, Season</creator><creatorcontrib>Wigfield, Donald C ; Tse, Season</creatorcontrib><description>The kinetics of oxidation of the mercurous ion by peroxidase have been measured by following the disappearance of mercurous ion using cold-vapour atomic absorption spectroscopy. Pseudo-first-order kinetics are observed with respect to mercurous ion, and the pseudo-first-order rate constants are linearly related to peroxidase concentration, showing first-order dependence on peroxidase. This behaviour is identical to oxidation of elemental mercury, and the second-order rate constant, 1.44 × 10 4  M −1 s −1 at 23 °C, is also, within experimental error, the same as that for elemental mercury oxidation. The data are interpreted in terms of peroxidase-induced disproportionation of the mercurous dimer, followed by two-electron oxidation of zero-valent mercury.</description><identifier>ISSN: 0008-4042</identifier><identifier>EISSN: 1480-3291</identifier><identifier>DOI: 10.1139/v86-162</identifier><identifier>CODEN: CJCHAG</identifier><language>eng</language><publisher>Ottawa, Canada: NRC Research Press</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; Oxidoreductases</subject><ispartof>Canadian journal of chemistry, 1986-05, Vol.64 (5), p.969-972</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://cdnsciencepub.com/doi/pdf/10.1139/v86-162$$EPDF$$P50$$Gnrcresearch$$H</linktopdf><linktohtml>$$Uhttps://cdnsciencepub.com/doi/full/10.1139/v86-162$$EHTML$$P50$$Gnrcresearch$$H</linktohtml><link.rule.ids>314,780,784,2932,27924,27925,64428,65234</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=8188223$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Wigfield, Donald C</creatorcontrib><creatorcontrib>Tse, Season</creatorcontrib><title>Oxidation of the mercurous ion by peroxidase</title><title>Canadian journal of chemistry</title><addtitle>Revue canadienne de chimie</addtitle><description>The kinetics of oxidation of the mercurous ion by peroxidase have been measured by following the disappearance of mercurous ion using cold-vapour atomic absorption spectroscopy. Pseudo-first-order kinetics are observed with respect to mercurous ion, and the pseudo-first-order rate constants are linearly related to peroxidase concentration, showing first-order dependence on peroxidase. This behaviour is identical to oxidation of elemental mercury, and the second-order rate constant, 1.44 × 10 4  M −1 s −1 at 23 °C, is also, within experimental error, the same as that for elemental mercury oxidation. The data are interpreted in terms of peroxidase-induced disproportionation of the mercurous dimer, followed by two-electron oxidation of zero-valent mercury.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Oxidoreductases</subject><issn>0008-4042</issn><issn>1480-3291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNp1z01Lw0AQBuBFFIxV_As5iIIYnZl87R6l-AWFXvQcNptZGmmTsJuK_ffd0tKD4GmY4eEdXiGuER4RU_X0I4sECzoREWYSkpQUnooIAGSSQUbn4sL777CWQHkkHua_baPHtu_i3sbjguMVO7N2_drHu2O9iQd2_Q55vhRnVi89Xx3mRHy9vnxO35PZ_O1j-jxLDEkak6bOQFpWlmpGSMusMGywbNBqzkkpVSqtGRuTgU6plJJyJAuNrSWBpSKdiLt9rnG9945tNbh2pd2mQqh2JatQsgolg7zZy0F7o5fW6c60_sglhnBKA7vds84Zx561M4sjOmRVQ2MDvP8f_n2-BZsna8g</recordid><startdate>19860501</startdate><enddate>19860501</enddate><creator>Wigfield, Donald C</creator><creator>Tse, Season</creator><general>NRC Research Press</general><general>National Research Council of Canada</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19860501</creationdate><title>Oxidation of the mercurous ion by peroxidase</title><author>Wigfield, Donald C ; Tse, Season</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-db408fe9f2be103746cec17d1fae5299979aae1dc40a327882512f0dfb820f263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Oxidoreductases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wigfield, Donald C</creatorcontrib><creatorcontrib>Tse, Season</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Canadian journal of chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wigfield, Donald C</au><au>Tse, Season</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oxidation of the mercurous ion by peroxidase</atitle><jtitle>Canadian journal of chemistry</jtitle><addtitle>Revue canadienne de chimie</addtitle><date>1986-05-01</date><risdate>1986</risdate><volume>64</volume><issue>5</issue><spage>969</spage><epage>972</epage><pages>969-972</pages><issn>0008-4042</issn><eissn>1480-3291</eissn><coden>CJCHAG</coden><abstract>The kinetics of oxidation of the mercurous ion by peroxidase have been measured by following the disappearance of mercurous ion using cold-vapour atomic absorption spectroscopy. Pseudo-first-order kinetics are observed with respect to mercurous ion, and the pseudo-first-order rate constants are linearly related to peroxidase concentration, showing first-order dependence on peroxidase. This behaviour is identical to oxidation of elemental mercury, and the second-order rate constant, 1.44 × 10 4  M −1 s −1 at 23 °C, is also, within experimental error, the same as that for elemental mercury oxidation. The data are interpreted in terms of peroxidase-induced disproportionation of the mercurous dimer, followed by two-electron oxidation of zero-valent mercury.</abstract><cop>Ottawa, Canada</cop><pub>NRC Research Press</pub><doi>10.1139/v86-162</doi><tpages>4</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0008-4042
ispartof Canadian journal of chemistry, 1986-05, Vol.64 (5), p.969-972
issn 0008-4042
1480-3291
language eng
recordid cdi_crossref_primary_10_1139_v86_162
source NRC Research Press; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry
subjects Analytical, structural and metabolic biochemistry
Biological and medical sciences
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Oxidoreductases
title Oxidation of the mercurous ion by peroxidase
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T04%3A43%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pascalfrancis_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Oxidation%20of%20the%20mercurous%20ion%20by%20peroxidase&rft.jtitle=Canadian%20journal%20of%20chemistry&rft.au=Wigfield,%20Donald%20C&rft.date=1986-05-01&rft.volume=64&rft.issue=5&rft.spage=969&rft.epage=972&rft.pages=969-972&rft.issn=0008-4042&rft.eissn=1480-3291&rft.coden=CJCHAG&rft_id=info:doi/10.1139/v86-162&rft_dat=%3Cpascalfrancis_cross%3E8188223%3C/pascalfrancis_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true