Genome-specific repetitive DNA and RAPD markers for genome identification in Elymus and Hordelymus
We have developed RFLP and RAPD markers specific for the genomes involved in the evolution of Elymus species, i.e., the St. Y, H, P, and W genomes. Two P genome specific repetitive DNA sequences, pAgc1 (350 bp) and pAgc30 (458 bp), and three W genome specific sequences, pAuv3 (221 bp), pAuv7 (200 bp...
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| Veröffentlicht in: | Genome 1998-02, Vol.41 (1), p.120-128 |
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| creator | Svitashev, S Bryngelsson, T Li, X Wang, R.R.C |
| description | We have developed RFLP and RAPD markers specific for the genomes involved in the evolution of Elymus species, i.e., the St. Y, H, P, and W genomes. Two P genome specific repetitive DNA sequences, pAgc1 (350 bp) and pAgc30 (458 bp), and three W genome specific sequences, pAuv3 (221 bp), pAuv7 (200 bp), and pAuv13 (207 bp), were isolated from the genomes of Agropyron cristatum and Australopyrum velutinum, respectively. Attempts to find Y genome specific sequences were not successful. Primary-structure analysis demonstrated that pAgc1 (P genome) and pAgc30 (P genome) share 81% similarity over a 227-bp stretch. The three W genome specific sequences were also highly homologous. Sequence comparison analysis revealed no homology to sequences in the EMBL- GenBank databases. Three to four genome-specific RAPD markers were found for each of the five genomes. Genome-specific bands were cloned and demonstrated to be mainly low-copy sequences present in venous Triticeae species. The RFLP and RAPD markers obtained, together with the previously described H and St genome specific clones pHch2 and pP1Taq2.5 and the Ns genome specific RAPD markers were used to investigate the genomic composition of a few Elymus species and Hordelymus europaeus, whose genome formulas were unknown. Our results demonstrate that only three of eight Elymus species examined (the tetraploid species Elymus grandis and the hexaploid species Elymus caesifolius and Elymus borianus) really belong to Elymus. |
| doi_str_mv | 10.1139/g97-108 |
| format | Article |
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Two P genome specific repetitive DNA sequences, pAgc1 (350 bp) and pAgc30 (458 bp), and three W genome specific sequences, pAuv3 (221 bp), pAuv7 (200 bp), and pAuv13 (207 bp), were isolated from the genomes of Agropyron cristatum and Australopyrum velutinum, respectively. Attempts to find Y genome specific sequences were not successful. Primary-structure analysis demonstrated that pAgc1 (P genome) and pAgc30 (P genome) share 81% similarity over a 227-bp stretch. The three W genome specific sequences were also highly homologous. Sequence comparison analysis revealed no homology to sequences in the EMBL- GenBank databases. Three to four genome-specific RAPD markers were found for each of the five genomes. Genome-specific bands were cloned and demonstrated to be mainly low-copy sequences present in venous Triticeae species. The RFLP and RAPD markers obtained, together with the previously described H and St genome specific clones pHch2 and pP1Taq2.5 and the Ns genome specific RAPD markers were used to investigate the genomic composition of a few Elymus species and Hordelymus europaeus, whose genome formulas were unknown. Our results demonstrate that only three of eight Elymus species examined (the tetraploid species Elymus grandis and the hexaploid species Elymus caesifolius and Elymus borianus) really belong to Elymus.</description><identifier>ISSN: 0831-2796</identifier><identifier>EISSN: 1480-3321</identifier><identifier>DOI: 10.1139/g97-108</identifier><identifier>PMID: 9549065</identifier><identifier>CODEN: GENOE3</identifier><language>eng</language><publisher>Ottawa, Canada: NRC Research Press</publisher><subject>Base Sequence ; Chromosomes ; Cloning, Molecular ; Deoxyribonucleic acid ; DNA ; DNA, Plant - genetics ; Edible Grain - classification ; Edible Grain - genetics ; Elymus ; Flowers & plants ; genbank/y14132 ; genbank/y14133 ; genbank/y14134 ; genbank/y14135 ; genbank/y14136 ; Genetic engineering ; Genetic Markers ; Genetics ; genome ; Genome, Plant ; Genomics ; Hybridization ; identification ; Molecular Sequence Data ; nucleotide sequences ; Poaceae ; Polymorphism, Restriction Fragment Length ; Polyploidy ; provenance ; Random Amplified Polymorphic DNA Technique ; repetitive sequences ; Repetitive Sequences, Nucleic Acid ; restriction fragment length polymorphism ; Sequence Homology, Nucleic Acid ; Species Specificity</subject><ispartof>Genome, 1998-02, Vol.41 (1), p.120-128</ispartof><rights>Copyright National Research Council of Canada Feb 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-28010f2828ceea6f5ca9857f04059dbaa60283eba5ae054b35f58201537c50483</citedby><cites>FETCH-LOGICAL-c424t-28010f2828ceea6f5ca9857f04059dbaa60283eba5ae054b35f58201537c50483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://cdnsciencepub.com/doi/pdf/10.1139/g97-108$$EPDF$$P50$$Gnrcresearch$$H</linktopdf><linktohtml>$$Uhttps://cdnsciencepub.com/doi/full/10.1139/g97-108$$EHTML$$P50$$Gnrcresearch$$H</linktohtml><link.rule.ids>314,776,780,2919,27901,27902,64401,64979</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9549065$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Svitashev, S</creatorcontrib><creatorcontrib>Bryngelsson, T</creatorcontrib><creatorcontrib>Li, X</creatorcontrib><creatorcontrib>Wang, R.R.C</creatorcontrib><title>Genome-specific repetitive DNA and RAPD markers for genome identification in Elymus and Hordelymus</title><title>Genome</title><addtitle>Génome</addtitle><description>We have developed RFLP and RAPD markers specific for the genomes involved in the evolution of Elymus species, i.e., the St. Y, H, P, and W genomes. Two P genome specific repetitive DNA sequences, pAgc1 (350 bp) and pAgc30 (458 bp), and three W genome specific sequences, pAuv3 (221 bp), pAuv7 (200 bp), and pAuv13 (207 bp), were isolated from the genomes of Agropyron cristatum and Australopyrum velutinum, respectively. Attempts to find Y genome specific sequences were not successful. Primary-structure analysis demonstrated that pAgc1 (P genome) and pAgc30 (P genome) share 81% similarity over a 227-bp stretch. The three W genome specific sequences were also highly homologous. Sequence comparison analysis revealed no homology to sequences in the EMBL- GenBank databases. Three to four genome-specific RAPD markers were found for each of the five genomes. Genome-specific bands were cloned and demonstrated to be mainly low-copy sequences present in venous Triticeae species. The RFLP and RAPD markers obtained, together with the previously described H and St genome specific clones pHch2 and pP1Taq2.5 and the Ns genome specific RAPD markers were used to investigate the genomic composition of a few Elymus species and Hordelymus europaeus, whose genome formulas were unknown. Our results demonstrate that only three of eight Elymus species examined (the tetraploid species Elymus grandis and the hexaploid species Elymus caesifolius and Elymus borianus) really belong to Elymus.</description><subject>Base Sequence</subject><subject>Chromosomes</subject><subject>Cloning, Molecular</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Plant - genetics</subject><subject>Edible Grain - classification</subject><subject>Edible Grain - genetics</subject><subject>Elymus</subject><subject>Flowers & plants</subject><subject>genbank/y14132</subject><subject>genbank/y14133</subject><subject>genbank/y14134</subject><subject>genbank/y14135</subject><subject>genbank/y14136</subject><subject>Genetic engineering</subject><subject>Genetic Markers</subject><subject>Genetics</subject><subject>genome</subject><subject>Genome, Plant</subject><subject>Genomics</subject><subject>Hybridization</subject><subject>identification</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>Poaceae</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Polyploidy</subject><subject>provenance</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>repetitive sequences</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>restriction fragment length polymorphism</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Species Specificity</subject><issn>0831-2796</issn><issn>1480-3321</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkV9rFDEUxYNY6raKn0AMPlQojN4kk5nkcWlrKxQVtc8hk7lZU3cmYzJT6Lfv7B8qKOJTEvidk3PvIeQlg3eMCf1-peuCgXpCFqxUUAjB2VOyACVYwWtdPSNHOd8CMBCaHZJDLUsNlVyQ5hL72GGRB3TBB0cTDjiGMdwhPf-0pLZv6dfll3Pa2fQTU6Y-JrraamhosR83IjuG2NPQ04v1fTflregqpha3z-fkwNt1xhf785jcfLj4fnZVXH--_Hi2vC5cycux4GpO57niyiHayktntZK1hxKkbhtrK-BKYGOlRZBlI6SXigOTonYSSiWOycnOd0jx14R5NF3IDtdr22Ocsqm1AtAa_guyquRC8g345g_wNk6pn4cwnIMEpnU1Q293kEsx54TeDCnM27o3DMymGzN3M983AV_t7aamw_aR25fxO1efXMKMNrkfj9DexAytn8HTf4N___p6B3sbjV2lkM3Nt3lvAliteS2keAC3h6wH</recordid><startdate>19980201</startdate><enddate>19980201</enddate><creator>Svitashev, S</creator><creator>Bryngelsson, T</creator><creator>Li, X</creator><creator>Wang, R.R.C</creator><general>NRC Research Press</general><general>Canadian Science Publishing NRC Research Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FQ</scope><scope>8FV</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M3G</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19980201</creationdate><title>Genome-specific repetitive DNA and RAPD markers for genome identification in Elymus and Hordelymus</title><author>Svitashev, S ; Bryngelsson, T ; Li, X ; Wang, R.R.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-28010f2828ceea6f5ca9857f04059dbaa60283eba5ae054b35f58201537c50483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Base Sequence</topic><topic>Chromosomes</topic><topic>Cloning, Molecular</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Plant - genetics</topic><topic>Edible Grain - classification</topic><topic>Edible Grain - genetics</topic><topic>Elymus</topic><topic>Flowers & plants</topic><topic>genbank/y14132</topic><topic>genbank/y14133</topic><topic>genbank/y14134</topic><topic>genbank/y14135</topic><topic>genbank/y14136</topic><topic>Genetic engineering</topic><topic>Genetic Markers</topic><topic>Genetics</topic><topic>genome</topic><topic>Genome, Plant</topic><topic>Genomics</topic><topic>Hybridization</topic><topic>identification</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>Poaceae</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Polyploidy</topic><topic>provenance</topic><topic>Random Amplified Polymorphic DNA Technique</topic><topic>repetitive sequences</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>restriction fragment length polymorphism</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Svitashev, S</creatorcontrib><creatorcontrib>Bryngelsson, T</creatorcontrib><creatorcontrib>Li, X</creatorcontrib><creatorcontrib>Wang, R.R.C</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Canadian Business & Current Affairs Database</collection><collection>Canadian Business & Current Affairs Database (Alumni Edition)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>CBCA Reference & Current Events</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genome</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Svitashev, S</au><au>Bryngelsson, T</au><au>Li, X</au><au>Wang, R.R.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome-specific repetitive DNA and RAPD markers for genome identification in Elymus and Hordelymus</atitle><jtitle>Genome</jtitle><addtitle>Génome</addtitle><date>1998-02-01</date><risdate>1998</risdate><volume>41</volume><issue>1</issue><spage>120</spage><epage>128</epage><pages>120-128</pages><issn>0831-2796</issn><eissn>1480-3321</eissn><coden>GENOE3</coden><abstract>We have developed RFLP and RAPD markers specific for the genomes involved in the evolution of Elymus species, i.e., the St. Y, H, P, and W genomes. Two P genome specific repetitive DNA sequences, pAgc1 (350 bp) and pAgc30 (458 bp), and three W genome specific sequences, pAuv3 (221 bp), pAuv7 (200 bp), and pAuv13 (207 bp), were isolated from the genomes of Agropyron cristatum and Australopyrum velutinum, respectively. Attempts to find Y genome specific sequences were not successful. Primary-structure analysis demonstrated that pAgc1 (P genome) and pAgc30 (P genome) share 81% similarity over a 227-bp stretch. The three W genome specific sequences were also highly homologous. Sequence comparison analysis revealed no homology to sequences in the EMBL- GenBank databases. Three to four genome-specific RAPD markers were found for each of the five genomes. Genome-specific bands were cloned and demonstrated to be mainly low-copy sequences present in venous Triticeae species. The RFLP and RAPD markers obtained, together with the previously described H and St genome specific clones pHch2 and pP1Taq2.5 and the Ns genome specific RAPD markers were used to investigate the genomic composition of a few Elymus species and Hordelymus europaeus, whose genome formulas were unknown. Our results demonstrate that only three of eight Elymus species examined (the tetraploid species Elymus grandis and the hexaploid species Elymus caesifolius and Elymus borianus) really belong to Elymus.</abstract><cop>Ottawa, Canada</cop><pub>NRC Research Press</pub><pmid>9549065</pmid><doi>10.1139/g97-108</doi><tpages>9</tpages></addata></record> |
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| source | NRC Research Press; MEDLINE |
| subjects | Base Sequence Chromosomes Cloning, Molecular Deoxyribonucleic acid DNA DNA, Plant - genetics Edible Grain - classification Edible Grain - genetics Elymus Flowers & plants genbank/y14132 genbank/y14133 genbank/y14134 genbank/y14135 genbank/y14136 Genetic engineering Genetic Markers Genetics genome Genome, Plant Genomics Hybridization identification Molecular Sequence Data nucleotide sequences Poaceae Polymorphism, Restriction Fragment Length Polyploidy provenance Random Amplified Polymorphic DNA Technique repetitive sequences Repetitive Sequences, Nucleic Acid restriction fragment length polymorphism Sequence Homology, Nucleic Acid Species Specificity |
| title | Genome-specific repetitive DNA and RAPD markers for genome identification in Elymus and Hordelymus |
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