A direct introduction of 18O isotopes into peptides and proteins for quantitative mass spectroscopy analysis
A method for direct introduction of 18 O isotopes into carboxyl groups of peptides and proteins via the exchange with H 2 18 O in the presence of TFA is described. The isotope label is sufficiently stable in a wide pH range. Since the compounds labeled by this method retain their physicochemical cha...
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| Veröffentlicht in: | Russian journal of bioorganic chemistry 2011-11, Vol.37 (6), p.719-731 |
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| container_title | Russian journal of bioorganic chemistry |
| container_volume | 37 |
| creator | Kozmin, Yu. P. Manoilov, A. V. Serebryakova, M. V. Mirgorodskaya, O. A. |
| description | A method for direct introduction of
18
O isotopes into carboxyl groups of peptides and proteins via the exchange with H
2
18
O in the presence of TFA is described. The isotope label is sufficiently stable in a wide pH range. Since the compounds labeled by this method retain their physicochemical characteristics, they can be used as an internal standard in quantitative assay of authentic compounds in the analyzed objects by means of mass spectrometry. This method is applicable to quantitative analysis of peptides and proteins in biological environments, as well as for quantitative kinetic studies of metabolism and enzyme activity. The quantitative analysis of polypeptides and proteins is combined with trypsinolysis. When necessary, the isotope label can be simultaneously introduced into all peptides and proteins in a control biosample, making it applicable as a standard for comparative analysis of experimental biosamples. |
| doi_str_mv | 10.1134/S1068162011060094 |
| format | Article |
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18
O isotopes into carboxyl groups of peptides and proteins via the exchange with H
2
18
O in the presence of TFA is described. The isotope label is sufficiently stable in a wide pH range. Since the compounds labeled by this method retain their physicochemical characteristics, they can be used as an internal standard in quantitative assay of authentic compounds in the analyzed objects by means of mass spectrometry. This method is applicable to quantitative analysis of peptides and proteins in biological environments, as well as for quantitative kinetic studies of metabolism and enzyme activity. The quantitative analysis of polypeptides and proteins is combined with trypsinolysis. When necessary, the isotope label can be simultaneously introduced into all peptides and proteins in a control biosample, making it applicable as a standard for comparative analysis of experimental biosamples.</description><identifier>ISSN: 1068-1620</identifier><identifier>EISSN: 1608-330X</identifier><identifier>DOI: 10.1134/S1068162011060094</identifier><language>eng</language><publisher>Dordrecht: SP MAIK Nauka/Interperiodica</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Bioorganic Chemistry ; Life Sciences ; Organic Chemistry</subject><ispartof>Russian journal of bioorganic chemistry, 2011-11, Vol.37 (6), p.719-731</ispartof><rights>Pleiades Publishing, Ltd. 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1334-ca60b3fe9e3a587dfeb1f3e74c5c952d29e466f645f3427cb73bd8bd1d1eae583</citedby><cites>FETCH-LOGICAL-c1334-ca60b3fe9e3a587dfeb1f3e74c5c952d29e466f645f3427cb73bd8bd1d1eae583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1068162011060094$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1068162011060094$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Kozmin, Yu. P.</creatorcontrib><creatorcontrib>Manoilov, A. V.</creatorcontrib><creatorcontrib>Serebryakova, M. V.</creatorcontrib><creatorcontrib>Mirgorodskaya, O. A.</creatorcontrib><title>A direct introduction of 18O isotopes into peptides and proteins for quantitative mass spectroscopy analysis</title><title>Russian journal of bioorganic chemistry</title><addtitle>Russ J Bioorg Chem</addtitle><description>A method for direct introduction of
18
O isotopes into carboxyl groups of peptides and proteins via the exchange with H
2
18
O in the presence of TFA is described. The isotope label is sufficiently stable in a wide pH range. Since the compounds labeled by this method retain their physicochemical characteristics, they can be used as an internal standard in quantitative assay of authentic compounds in the analyzed objects by means of mass spectrometry. This method is applicable to quantitative analysis of peptides and proteins in biological environments, as well as for quantitative kinetic studies of metabolism and enzyme activity. The quantitative analysis of polypeptides and proteins is combined with trypsinolysis. When necessary, the isotope label can be simultaneously introduced into all peptides and proteins in a control biosample, making it applicable as a standard for comparative analysis of experimental biosamples.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bioorganic Chemistry</subject><subject>Life Sciences</subject><subject>Organic Chemistry</subject><issn>1068-1620</issn><issn>1608-330X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9UMtKAzEUDaJgrX6Au_zAaO4k81qW4gsKXajgbsgkN5LSTsbcVOjfm1J3gqt7LufB4TB2C-IOQKr7VxB1C3UpIAMhOnXGZlCLtpBSfJxnnOniyF-yK6KNECBE1c7YdsGtj2gS92OKwe5N8mHkwXFo19xTSGFCOpKBTzglb_OnR8unGBL6kbgLkX_t9Zh80sl_I99pIk5TzoyBTJgOWa-3B_J0zS6c3hLe_N45e398eFs-F6v108tysSoMSKkKo2sxSIcdSl21jXU4gJPYKFOZript2aGqa1eryklVNmZo5GDbwYIF1Fi1cs7glGtyA4ro-in6nY6HHkR_nKv_M1f2lCcPZe34ibHfhH3Mxekf0w9JeG8x</recordid><startdate>201111</startdate><enddate>201111</enddate><creator>Kozmin, Yu. P.</creator><creator>Manoilov, A. V.</creator><creator>Serebryakova, M. V.</creator><creator>Mirgorodskaya, O. A.</creator><general>SP MAIK Nauka/Interperiodica</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>201111</creationdate><title>A direct introduction of 18O isotopes into peptides and proteins for quantitative mass spectroscopy analysis</title><author>Kozmin, Yu. P. ; Manoilov, A. V. ; Serebryakova, M. V. ; Mirgorodskaya, O. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1334-ca60b3fe9e3a587dfeb1f3e74c5c952d29e466f645f3427cb73bd8bd1d1eae583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bioorganic Chemistry</topic><topic>Life Sciences</topic><topic>Organic Chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kozmin, Yu. P.</creatorcontrib><creatorcontrib>Manoilov, A. V.</creatorcontrib><creatorcontrib>Serebryakova, M. V.</creatorcontrib><creatorcontrib>Mirgorodskaya, O. A.</creatorcontrib><collection>CrossRef</collection><jtitle>Russian journal of bioorganic chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kozmin, Yu. P.</au><au>Manoilov, A. V.</au><au>Serebryakova, M. V.</au><au>Mirgorodskaya, O. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A direct introduction of 18O isotopes into peptides and proteins for quantitative mass spectroscopy analysis</atitle><jtitle>Russian journal of bioorganic chemistry</jtitle><stitle>Russ J Bioorg Chem</stitle><date>2011-11</date><risdate>2011</risdate><volume>37</volume><issue>6</issue><spage>719</spage><epage>731</epage><pages>719-731</pages><issn>1068-1620</issn><eissn>1608-330X</eissn><abstract>A method for direct introduction of
18
O isotopes into carboxyl groups of peptides and proteins via the exchange with H
2
18
O in the presence of TFA is described. The isotope label is sufficiently stable in a wide pH range. Since the compounds labeled by this method retain their physicochemical characteristics, they can be used as an internal standard in quantitative assay of authentic compounds in the analyzed objects by means of mass spectrometry. This method is applicable to quantitative analysis of peptides and proteins in biological environments, as well as for quantitative kinetic studies of metabolism and enzyme activity. The quantitative analysis of polypeptides and proteins is combined with trypsinolysis. When necessary, the isotope label can be simultaneously introduced into all peptides and proteins in a control biosample, making it applicable as a standard for comparative analysis of experimental biosamples.</abstract><cop>Dordrecht</cop><pub>SP MAIK Nauka/Interperiodica</pub><doi>10.1134/S1068162011060094</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Russian journal of bioorganic chemistry, 2011-11, Vol.37 (6), p.719-731 |
| issn | 1068-1620 1608-330X |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Life Sciences Organic Chemistry |
| title | A direct introduction of 18O isotopes into peptides and proteins for quantitative mass spectroscopy analysis |
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