Nanocomplexes of recombinant proteins and polysialic acid: Preparation, characteristics, and biological activity
A preparation of nanocomplexes containing recombinant proteins (interferons α2b and β1b, insulin, and human granulocyte colony stimulating factor) and natural polysialic acid (PSA) has been described. The incorporation of protein into the complex changes its electrophoretic mobility. Atomic force mi...
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| Veröffentlicht in: | Russian journal of bioorganic chemistry 2009-05, Vol.35 (3), p.320-325 |
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| container_title | Russian journal of bioorganic chemistry |
| container_volume | 35 |
| creator | Bezuglov, V. V. Gretskaya, N. M. Klinov, D. V. Bobrov, M. Yu Shibanova, E. D. Akimov, M. G. Fomina-Ageeva, E. V. Zinchenko, G. N. Bairamashvili, D. I. Miroshnikov, A. I. |
| description | A preparation of nanocomplexes containing recombinant proteins (interferons α2b and β1b, insulin, and human granulocyte colony stimulating factor) and natural polysialic acid (PSA) has been described. The incorporation of protein into the complex changes its electrophoretic mobility. Atomic force microscopy reveals the average size of 23-kD insulin complexes with PSA of 10–20 nm and demonstrates that more than 60% of glycopolymer molecules carry a single protein molecule. Experiments with cultured cells show that cytokines bound to polysialic acid retain their ability to regulate cell proliferation. Insulin bound to PSA has a prolonged hypoglycemic effect in vivo. |
| doi_str_mv | 10.1134/S1068162009030066 |
| format | Article |
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Experiments with cultured cells show that cytokines bound to polysialic acid retain their ability to regulate cell proliferation. Insulin bound to PSA has a prolonged hypoglycemic effect in vivo.</description><identifier>ISSN: 1068-1620</identifier><identifier>EISSN: 1608-330X</identifier><identifier>DOI: 10.1134/S1068162009030066</identifier><language>eng</language><publisher>Dordrecht: SP MAIK Nauka/Interperiodica</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Bioorganic Chemistry ; Life Sciences ; Organic Chemistry</subject><ispartof>Russian journal of bioorganic chemistry, 2009-05, Vol.35 (3), p.320-325</ispartof><rights>Pleiades Publishing, Ltd. 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c288t-25cfbefd7bb78a8b526009edc2fc5e33a8cb2f81f1d384fa36cd6263514189543</citedby><cites>FETCH-LOGICAL-c288t-25cfbefd7bb78a8b526009edc2fc5e33a8cb2f81f1d384fa36cd6263514189543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1068162009030066$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1068162009030066$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Bezuglov, V. 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Atomic force microscopy reveals the average size of 23-kD insulin complexes with PSA of 10–20 nm and demonstrates that more than 60% of glycopolymer molecules carry a single protein molecule. Experiments with cultured cells show that cytokines bound to polysialic acid retain their ability to regulate cell proliferation. Insulin bound to PSA has a prolonged hypoglycemic effect in vivo.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bioorganic Chemistry</subject><subject>Life Sciences</subject><subject>Organic Chemistry</subject><issn>1068-1620</issn><issn>1608-330X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp9kN1KAzEQhYMoWKsP4F0eoKv52U1T76RoFYoKKni3JLNJTdkmSxLFvr2p9U7was5w5htmDkLnlFxQyuvLZ0qEpIIRMiOcECEO0IgKIivOydth0cWudv4xOklpTQglpJEjNDwoHyBsht58mYSDxdGUVjuvfMZDDNk4n7DyHR5Cv01O9Q6wAtdd4adoBhVVdsFPMLwXCdlEl7KDNPlBtAt9WDlQfUGy-3R5e4qOrOqTOfutY_R6e_Myv6uWj4v7-fWyAiZlrlgDVhvbTbWeSiV1w0R5zXTALDSGcyVBMyuppR2XtVVcQCeY4A2tqZw1NR8jut8LMaQUjW2H6DYqbltK2l1k7Z_ICsP2TCqzfmViuw4f0Zcz_4G-AaxlcHw</recordid><startdate>20090501</startdate><enddate>20090501</enddate><creator>Bezuglov, V. V.</creator><creator>Gretskaya, N. 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I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanocomplexes of recombinant proteins and polysialic acid: Preparation, characteristics, and biological activity</atitle><jtitle>Russian journal of bioorganic chemistry</jtitle><stitle>Russ J Bioorg Chem</stitle><date>2009-05-01</date><risdate>2009</risdate><volume>35</volume><issue>3</issue><spage>320</spage><epage>325</epage><pages>320-325</pages><issn>1068-1620</issn><eissn>1608-330X</eissn><abstract>A preparation of nanocomplexes containing recombinant proteins (interferons α2b and β1b, insulin, and human granulocyte colony stimulating factor) and natural polysialic acid (PSA) has been described. The incorporation of protein into the complex changes its electrophoretic mobility. Atomic force microscopy reveals the average size of 23-kD insulin complexes with PSA of 10–20 nm and demonstrates that more than 60% of glycopolymer molecules carry a single protein molecule. 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| ispartof | Russian journal of bioorganic chemistry, 2009-05, Vol.35 (3), p.320-325 |
| issn | 1068-1620 1608-330X |
| language | eng |
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| source | SpringerNature Journals |
| subjects | Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Life Sciences Organic Chemistry |
| title | Nanocomplexes of recombinant proteins and polysialic acid: Preparation, characteristics, and biological activity |
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