Polymorphisms, Molecular Characteristics of LGB Gene in River and Swamp Buffalo (Bubalus bubalis)
Beta-lactoglobulin (β-LG) is the major whey protein associated with lactation traits in ruminants and has a potential impact on human health. So far, the polymorphisms and molecular characteristics of buffalo LGB gene have not been well understood. In this study, the complete coding sequence (CDS) o...
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| creator | Fan, X. Y. Guo, W. B. Qiu, L. H. Zhang, Y. Y. Miao, Y. W. |
| description | Beta-lactoglobulin (β-LG) is the major whey protein associated with lactation traits in ruminants and has a potential impact on human health. So far, the polymorphisms and molecular characteristics of buffalo
LGB
gene have not been well understood. In this study, the complete coding sequence (CDS) of buffalo
LGB
were isolated and its polymorphisms were detected using direct sequencing of PCR product. The CDS of
LGB
gene for river and swamp buffalo was 543 nucleotides in length, encoding a peptide with 180 amino acid residues. A total of eight single nucleotide polymorphisms (SNPs) was identified in the CDS of
LGB
gene in two types of buffalo. Among them, c.46G>A and c.131C>T were non-synonymous, which led to an amino acid substitution from Ala to Thr at the 16th position of the signal peptide and a p.Ala26Val substitution in the mature peptide of buffalo β-LG, respectively. The prediction showed that the p.Ala26Val may affect the function of buffalo β-LG. Seven
LGB
haplotypes were defined based on the SNPs observed in buffalo, and accordingly, two variants and two synonymous variants of buffalo β-LG were inferred, named variant A, A
1
, A
2
and B, respectively. All the variants determined in buffalo did not exist in the animals of
Bos
genus. The sequence alignment of β-LG mature peptide showed that there were two different amino acids (p.Ile1Leu and p.Val162Ile) between buffalo and the animals of
Bos
genus. The signal peptide of buffalo β-LG was two amino acids longer than that of
Bos
genus and there was an differential amino acid at the 13th position of signal peptide, which may lead to different functions of signal peptide between buffalo and
Bos
genus. The hydrophilicity of buffalo β-LG variants A and B were slightly different, and their physicochemical characteristics were similar to those of cattle β-LG B. The mature peptides of β-LG in buffalo and
Bos
genus had a lipocalin domain, which revealed that they had the same function. This study will give us new insights into the variation, physicochemical characteristics and biological function of buffalo
LGB
gene. |
| doi_str_mv | 10.1134/S1022795421020034 |
| format | Article |
| fullrecord | <record><control><sourceid>crossref_sprin</sourceid><recordid>TN_cdi_crossref_primary_10_1134_S1022795421020034</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_1134_S1022795421020034</sourcerecordid><originalsourceid>FETCH-LOGICAL-c288t-edc9f1cd8a76b03e4998ea4f0ddc3bf3dd107956b9fdff84a4c579176a7181f63</originalsourceid><addsrcrecordid>eNp9kE9LAzEUxIMoWKsfwFuOCq6-bNJscrRFq1BRrJ6XbP7YlN1NSbpKv70pehM8zcDvzWMYhM4JXBNC2c2SQFlWcsLKbAAoO0AjwkEUlHJ5mH3GxZ4fo5OU1gAEgNMRUi-h3XUhblY-dekKP4XW6qFVEc9WKiq9tdGnrdcJB4cX8yme295i3-NX_2kjVr3Byy_VbfB0cE61AV9Mh0a1Q8LNXn26PEVHGSR79qtj9H5_9zZ7KBbP88fZ7aLQpRDbwhotHdFGqIo3QC2TUljFHBijaeOoMQRyf95IZ5wTTDE9qSSpuKqIII7TMSI_f3UMKUXr6k30nYq7mkC936j-s1HOlD-ZlG_7DxvrdRhin2v-E_oGHRdpGA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Polymorphisms, Molecular Characteristics of LGB Gene in River and Swamp Buffalo (Bubalus bubalis)</title><source>SpringerNature Complete Journals</source><creator>Fan, X. Y. ; Guo, W. B. ; Qiu, L. H. ; Zhang, Y. Y. ; Miao, Y. W.</creator><creatorcontrib>Fan, X. Y. ; Guo, W. B. ; Qiu, L. H. ; Zhang, Y. Y. ; Miao, Y. W.</creatorcontrib><description>Beta-lactoglobulin (β-LG) is the major whey protein associated with lactation traits in ruminants and has a potential impact on human health. So far, the polymorphisms and molecular characteristics of buffalo
LGB
gene have not been well understood. In this study, the complete coding sequence (CDS) of buffalo
LGB
were isolated and its polymorphisms were detected using direct sequencing of PCR product. The CDS of
LGB
gene for river and swamp buffalo was 543 nucleotides in length, encoding a peptide with 180 amino acid residues. A total of eight single nucleotide polymorphisms (SNPs) was identified in the CDS of
LGB
gene in two types of buffalo. Among them, c.46G>A and c.131C>T were non-synonymous, which led to an amino acid substitution from Ala to Thr at the 16th position of the signal peptide and a p.Ala26Val substitution in the mature peptide of buffalo β-LG, respectively. The prediction showed that the p.Ala26Val may affect the function of buffalo β-LG. Seven
LGB
haplotypes were defined based on the SNPs observed in buffalo, and accordingly, two variants and two synonymous variants of buffalo β-LG were inferred, named variant A, A
1
, A
2
and B, respectively. All the variants determined in buffalo did not exist in the animals of
Bos
genus. The sequence alignment of β-LG mature peptide showed that there were two different amino acids (p.Ile1Leu and p.Val162Ile) between buffalo and the animals of
Bos
genus. The signal peptide of buffalo β-LG was two amino acids longer than that of
Bos
genus and there was an differential amino acid at the 13th position of signal peptide, which may lead to different functions of signal peptide between buffalo and
Bos
genus. The hydrophilicity of buffalo β-LG variants A and B were slightly different, and their physicochemical characteristics were similar to those of cattle β-LG B. The mature peptides of β-LG in buffalo and
Bos
genus had a lipocalin domain, which revealed that they had the same function. This study will give us new insights into the variation, physicochemical characteristics and biological function of buffalo
LGB
gene.</description><identifier>ISSN: 1022-7954</identifier><identifier>EISSN: 1608-3369</identifier><identifier>DOI: 10.1134/S1022795421020034</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Animal Genetics ; Animal Genetics and Genomics ; Biomedical and Life Sciences ; Biomedicine ; Human Genetics ; Microbial Genetics and Genomics</subject><ispartof>Russian journal of genetics, 2021-02, Vol.57 (2), p.204-212</ispartof><rights>Pleiades Publishing, Inc. 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c288t-edc9f1cd8a76b03e4998ea4f0ddc3bf3dd107956b9fdff84a4c579176a7181f63</citedby><cites>FETCH-LOGICAL-c288t-edc9f1cd8a76b03e4998ea4f0ddc3bf3dd107956b9fdff84a4c579176a7181f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1022795421020034$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1022795421020034$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Fan, X. Y.</creatorcontrib><creatorcontrib>Guo, W. B.</creatorcontrib><creatorcontrib>Qiu, L. H.</creatorcontrib><creatorcontrib>Zhang, Y. Y.</creatorcontrib><creatorcontrib>Miao, Y. W.</creatorcontrib><title>Polymorphisms, Molecular Characteristics of LGB Gene in River and Swamp Buffalo (Bubalus bubalis)</title><title>Russian journal of genetics</title><addtitle>Russ J Genet</addtitle><description>Beta-lactoglobulin (β-LG) is the major whey protein associated with lactation traits in ruminants and has a potential impact on human health. So far, the polymorphisms and molecular characteristics of buffalo
LGB
gene have not been well understood. In this study, the complete coding sequence (CDS) of buffalo
LGB
were isolated and its polymorphisms were detected using direct sequencing of PCR product. The CDS of
LGB
gene for river and swamp buffalo was 543 nucleotides in length, encoding a peptide with 180 amino acid residues. A total of eight single nucleotide polymorphisms (SNPs) was identified in the CDS of
LGB
gene in two types of buffalo. Among them, c.46G>A and c.131C>T were non-synonymous, which led to an amino acid substitution from Ala to Thr at the 16th position of the signal peptide and a p.Ala26Val substitution in the mature peptide of buffalo β-LG, respectively. The prediction showed that the p.Ala26Val may affect the function of buffalo β-LG. Seven
LGB
haplotypes were defined based on the SNPs observed in buffalo, and accordingly, two variants and two synonymous variants of buffalo β-LG were inferred, named variant A, A
1
, A
2
and B, respectively. All the variants determined in buffalo did not exist in the animals of
Bos
genus. The sequence alignment of β-LG mature peptide showed that there were two different amino acids (p.Ile1Leu and p.Val162Ile) between buffalo and the animals of
Bos
genus. The signal peptide of buffalo β-LG was two amino acids longer than that of
Bos
genus and there was an differential amino acid at the 13th position of signal peptide, which may lead to different functions of signal peptide between buffalo and
Bos
genus. The hydrophilicity of buffalo β-LG variants A and B were slightly different, and their physicochemical characteristics were similar to those of cattle β-LG B. The mature peptides of β-LG in buffalo and
Bos
genus had a lipocalin domain, which revealed that they had the same function. This study will give us new insights into the variation, physicochemical characteristics and biological function of buffalo
LGB
gene.</description><subject>Animal Genetics</subject><subject>Animal Genetics and Genomics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Human Genetics</subject><subject>Microbial Genetics and Genomics</subject><issn>1022-7954</issn><issn>1608-3369</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kE9LAzEUxIMoWKsfwFuOCq6-bNJscrRFq1BRrJ6XbP7YlN1NSbpKv70pehM8zcDvzWMYhM4JXBNC2c2SQFlWcsLKbAAoO0AjwkEUlHJ5mH3GxZ4fo5OU1gAEgNMRUi-h3XUhblY-dekKP4XW6qFVEc9WKiq9tdGnrdcJB4cX8yme295i3-NX_2kjVr3Byy_VbfB0cE61AV9Mh0a1Q8LNXn26PEVHGSR79qtj9H5_9zZ7KBbP88fZ7aLQpRDbwhotHdFGqIo3QC2TUljFHBijaeOoMQRyf95IZ5wTTDE9qSSpuKqIII7TMSI_f3UMKUXr6k30nYq7mkC936j-s1HOlD-ZlG_7DxvrdRhin2v-E_oGHRdpGA</recordid><startdate>20210201</startdate><enddate>20210201</enddate><creator>Fan, X. Y.</creator><creator>Guo, W. B.</creator><creator>Qiu, L. H.</creator><creator>Zhang, Y. Y.</creator><creator>Miao, Y. W.</creator><general>Pleiades Publishing</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20210201</creationdate><title>Polymorphisms, Molecular Characteristics of LGB Gene in River and Swamp Buffalo (Bubalus bubalis)</title><author>Fan, X. Y. ; Guo, W. B. ; Qiu, L. H. ; Zhang, Y. Y. ; Miao, Y. W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c288t-edc9f1cd8a76b03e4998ea4f0ddc3bf3dd107956b9fdff84a4c579176a7181f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animal Genetics</topic><topic>Animal Genetics and Genomics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Human Genetics</topic><topic>Microbial Genetics and Genomics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fan, X. Y.</creatorcontrib><creatorcontrib>Guo, W. B.</creatorcontrib><creatorcontrib>Qiu, L. H.</creatorcontrib><creatorcontrib>Zhang, Y. Y.</creatorcontrib><creatorcontrib>Miao, Y. W.</creatorcontrib><collection>CrossRef</collection><jtitle>Russian journal of genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fan, X. Y.</au><au>Guo, W. B.</au><au>Qiu, L. H.</au><au>Zhang, Y. Y.</au><au>Miao, Y. W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polymorphisms, Molecular Characteristics of LGB Gene in River and Swamp Buffalo (Bubalus bubalis)</atitle><jtitle>Russian journal of genetics</jtitle><stitle>Russ J Genet</stitle><date>2021-02-01</date><risdate>2021</risdate><volume>57</volume><issue>2</issue><spage>204</spage><epage>212</epage><pages>204-212</pages><issn>1022-7954</issn><eissn>1608-3369</eissn><abstract>Beta-lactoglobulin (β-LG) is the major whey protein associated with lactation traits in ruminants and has a potential impact on human health. So far, the polymorphisms and molecular characteristics of buffalo
LGB
gene have not been well understood. In this study, the complete coding sequence (CDS) of buffalo
LGB
were isolated and its polymorphisms were detected using direct sequencing of PCR product. The CDS of
LGB
gene for river and swamp buffalo was 543 nucleotides in length, encoding a peptide with 180 amino acid residues. A total of eight single nucleotide polymorphisms (SNPs) was identified in the CDS of
LGB
gene in two types of buffalo. Among them, c.46G>A and c.131C>T were non-synonymous, which led to an amino acid substitution from Ala to Thr at the 16th position of the signal peptide and a p.Ala26Val substitution in the mature peptide of buffalo β-LG, respectively. The prediction showed that the p.Ala26Val may affect the function of buffalo β-LG. Seven
LGB
haplotypes were defined based on the SNPs observed in buffalo, and accordingly, two variants and two synonymous variants of buffalo β-LG were inferred, named variant A, A
1
, A
2
and B, respectively. All the variants determined in buffalo did not exist in the animals of
Bos
genus. The sequence alignment of β-LG mature peptide showed that there were two different amino acids (p.Ile1Leu and p.Val162Ile) between buffalo and the animals of
Bos
genus. The signal peptide of buffalo β-LG was two amino acids longer than that of
Bos
genus and there was an differential amino acid at the 13th position of signal peptide, which may lead to different functions of signal peptide between buffalo and
Bos
genus. The hydrophilicity of buffalo β-LG variants A and B were slightly different, and their physicochemical characteristics were similar to those of cattle β-LG B. The mature peptides of β-LG in buffalo and
Bos
genus had a lipocalin domain, which revealed that they had the same function. This study will give us new insights into the variation, physicochemical characteristics and biological function of buffalo
LGB
gene.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S1022795421020034</doi><tpages>9</tpages></addata></record> |
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| source | SpringerNature Complete Journals |
| subjects | Animal Genetics Animal Genetics and Genomics Biomedical and Life Sciences Biomedicine Human Genetics Microbial Genetics and Genomics |
| title | Polymorphisms, Molecular Characteristics of LGB Gene in River and Swamp Buffalo (Bubalus bubalis) |
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