Somatic embryogenesis of two new Panax ginseng cultivars, Yun-Poong and Chun-Poong
Somatic embryogenesis from single cells is important for normal plant regeneration of ginseng. Cotyledon explants from zygotic embryos of two new ginseng cultivars, Chun-Poong and Yun-Poong, produced somatic embryos on Murashige and Skoog (MS) basal medium and MS medium containing growth regulators....
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| Veröffentlicht in: | Russian journal of plant physiology 2010-03, Vol.57 (2), p.283-289 |
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| container_title | Russian journal of plant physiology |
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| creator | Kim, Y. J Kim, M. K Shim, J. S Pulla, R. K Yang, D. C |
| description | Somatic embryogenesis from single cells is important for normal plant regeneration of ginseng. Cotyledon explants from zygotic embryos of two new ginseng cultivars, Chun-Poong and Yun-Poong, produced somatic embryos on Murashige and Skoog (MS) basal medium and MS medium containing growth regulators. The highest frequency of single somatic embryo formation was obtained when cotyledon explants were excised from premature (cultured for 1 day) zygotic embryos (about 6 mm in length) of both cvs. Chun-Poong and Yun-Poong and then cultured on MS medium supplemented with 7% sucrose. The frequency of single somatic embryo formation was strongly enhanced when Chun-Poong cotyledons were subjected to plasmolysis with 0.1-0.5 M sucrose for 24 h and Yun-Poong cotyledons to plasmolysis with 1.0 M sucrose for 24 h and then cultured on MS medium with 2,4-D. |
| doi_str_mv | 10.1134/S1021443710020172 |
| format | Article |
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J ; Kim, M. K ; Shim, J. S ; Pulla, R. K ; Yang, D. C</creator><creatorcontrib>Kim, Y. J ; Kim, M. K ; Shim, J. S ; Pulla, R. K ; Yang, D. C</creatorcontrib><description>Somatic embryogenesis from single cells is important for normal plant regeneration of ginseng. Cotyledon explants from zygotic embryos of two new ginseng cultivars, Chun-Poong and Yun-Poong, produced somatic embryos on Murashige and Skoog (MS) basal medium and MS medium containing growth regulators. The highest frequency of single somatic embryo formation was obtained when cotyledon explants were excised from premature (cultured for 1 day) zygotic embryos (about 6 mm in length) of both cvs. Chun-Poong and Yun-Poong and then cultured on MS medium supplemented with 7% sucrose. The frequency of single somatic embryo formation was strongly enhanced when Chun-Poong cotyledons were subjected to plasmolysis with 0.1-0.5 M sucrose for 24 h and Yun-Poong cotyledons to plasmolysis with 1.0 M sucrose for 24 h and then cultured on MS medium with 2,4-D.</description><identifier>ISSN: 1021-4437</identifier><identifier>EISSN: 1608-3407</identifier><identifier>DOI: 10.1134/S1021443710020172</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : SP MAIK Nauka/Interperiodica</publisher><subject>2,4-D ; Biomedical and Life Sciences ; cotyledons ; cultivars ; culture media ; explants ; growth regulators ; Life Sciences ; Panax ginseng ; Plant Physiology ; Plant Sciences ; plasmolysis ; Research Papers ; somatic embryogenesis ; somatic embryos ; sucrose</subject><ispartof>Russian journal of plant physiology, 2010-03, Vol.57 (2), p.283-289</ispartof><rights>Pleiades Publishing, Ltd. 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-cfa79c9c5609cf33bb70a73b0211667632e236455450f903be976ff289fb24973</citedby><cites>FETCH-LOGICAL-c343t-cfa79c9c5609cf33bb70a73b0211667632e236455450f903be976ff289fb24973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1021443710020172$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1021443710020172$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27907,27908,41471,42540,51302</link.rule.ids></links><search><creatorcontrib>Kim, Y. 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The frequency of single somatic embryo formation was strongly enhanced when Chun-Poong cotyledons were subjected to plasmolysis with 0.1-0.5 M sucrose for 24 h and Yun-Poong cotyledons to plasmolysis with 1.0 M sucrose for 24 h and then cultured on MS medium with 2,4-D.</description><subject>2,4-D</subject><subject>Biomedical and Life Sciences</subject><subject>cotyledons</subject><subject>cultivars</subject><subject>culture media</subject><subject>explants</subject><subject>growth regulators</subject><subject>Life Sciences</subject><subject>Panax ginseng</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>plasmolysis</subject><subject>Research Papers</subject><subject>somatic embryogenesis</subject><subject>somatic embryos</subject><subject>sucrose</subject><issn>1021-4437</issn><issn>1608-3407</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp9kMtOwzAQRSMEEqXwAazwihWB8SN2skQVL6kSFaULVpZj7JCqtYudUPr3GAVWSKzmce8ZzUyWnWK4xJiyqzkGghmjAgMQwILsZSPMocwpA7Gf8iTn3_phdhTjEiD5eDHKnuZ-rbpWI7Ouw843xpnYRuQt6rYeObNFM-XUJ2paF41rkO5XXfuhQrxAL73LZ96npnKvaPL2Wx5nB1atojn5ieNscXvzPLnPp493D5Praa4po12urRKVrnTBodKW0roWoASt06KYc8EpMYRyVhSsAFsBrU0luLWkrGxNWCXoODsf5m6Cf-9N7OS6jdqsVsoZ30fJeFHykkIy4sGog48xGCs3oV2rsJMY5Pf35J_vJYYMTExe15ggl74PLt3zL3Q2QFZ5qZrQRrmYJ4kCLglwKOgXsMN41w</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>Kim, Y. 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K</creatorcontrib><creatorcontrib>Yang, D. C</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Russian journal of plant physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Y. J</au><au>Kim, M. K</au><au>Shim, J. S</au><au>Pulla, R. K</au><au>Yang, D. 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The highest frequency of single somatic embryo formation was obtained when cotyledon explants were excised from premature (cultured for 1 day) zygotic embryos (about 6 mm in length) of both cvs. Chun-Poong and Yun-Poong and then cultured on MS medium supplemented with 7% sucrose. The frequency of single somatic embryo formation was strongly enhanced when Chun-Poong cotyledons were subjected to plasmolysis with 0.1-0.5 M sucrose for 24 h and Yun-Poong cotyledons to plasmolysis with 1.0 M sucrose for 24 h and then cultured on MS medium with 2,4-D.</abstract><cop>Dordrecht</cop><pub>Dordrecht : SP MAIK Nauka/Interperiodica</pub><doi>10.1134/S1021443710020172</doi><tpages>7</tpages></addata></record> |
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| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | 2,4-D Biomedical and Life Sciences cotyledons cultivars culture media explants growth regulators Life Sciences Panax ginseng Plant Physiology Plant Sciences plasmolysis Research Papers somatic embryogenesis somatic embryos sucrose |
| title | Somatic embryogenesis of two new Panax ginseng cultivars, Yun-Poong and Chun-Poong |
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