Hemoglobin-catalyzed fluorometric method for the determination of glutathione
A new spectrofluorometric method for the determination of glutathione based on the reaction catalyzed by hemoglobin was reported. The reaction product gave a highly fluorescent intensity with the excitation and emission wavelengths of 320.0 nm and 413.0 nm, respectively. The optimum experimental con...
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| Veröffentlicht in: | Optics and spectroscopy 2016, Vol.120 (1), p.164-170 |
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| container_title | Optics and spectroscopy |
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| creator | Wang, Ruiqiang Tang, Lin Li, Hua Wang, Yi Gou, Rong Guo, Yuanyuan Fang, Yudong Chen, Fengmei |
| description | A new spectrofluorometric method for the determination of glutathione based on the reaction catalyzed by hemoglobin was reported. The reaction product gave a highly fluorescent intensity with the excitation and emission wavelengths of 320.0 nm and 413.0 nm, respectively. The optimum experimental conditions were investigated. Results showed that low concentration glutathione enhanced the fluorescence intensity significantly. The line ranges were 1.0 × 10
–6
–1.0 × 10
–5
mol L
–1
of glutathione and 6.0 × 10
-10
mol L
-1
–1.0 × 10
-8
mol L
-1
, respectively. The detection limit was calculated to be 1.1 × 10
-11
mol L
-1
. The recovery test by the standard addition method gave values in the range of 90.78%-102.20%. This method was used for the determination of glutathione in synthetic and real samples with satisfactory results. |
| doi_str_mv | 10.1134/S0030400X15120218 |
| format | Article |
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–6
–1.0 × 10
–5
mol L
–1
of glutathione and 6.0 × 10
-10
mol L
-1
–1.0 × 10
-8
mol L
-1
, respectively. The detection limit was calculated to be 1.1 × 10
-11
mol L
-1
. The recovery test by the standard addition method gave values in the range of 90.78%-102.20%. This method was used for the determination of glutathione in synthetic and real samples with satisfactory results.</description><identifier>ISSN: 0030-400X</identifier><identifier>EISSN: 1562-6911</identifier><identifier>DOI: 10.1134/S0030400X15120218</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Geometrical and Applied Optics ; Lasers ; Optical Devices ; Optics ; Photonics ; Physics ; Physics and Astronomy</subject><ispartof>Optics and spectroscopy, 2016, Vol.120 (1), p.164-170</ispartof><rights>Pleiades Publishing, Ltd. 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c288t-d0db38ef95ccf39562306e21ddca862b5baf052895734310145802249e9090013</citedby><cites>FETCH-LOGICAL-c288t-d0db38ef95ccf39562306e21ddca862b5baf052895734310145802249e9090013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0030400X15120218$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0030400X15120218$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Wang, Ruiqiang</creatorcontrib><creatorcontrib>Tang, Lin</creatorcontrib><creatorcontrib>Li, Hua</creatorcontrib><creatorcontrib>Wang, Yi</creatorcontrib><creatorcontrib>Gou, Rong</creatorcontrib><creatorcontrib>Guo, Yuanyuan</creatorcontrib><creatorcontrib>Fang, Yudong</creatorcontrib><creatorcontrib>Chen, Fengmei</creatorcontrib><title>Hemoglobin-catalyzed fluorometric method for the determination of glutathione</title><title>Optics and spectroscopy</title><addtitle>Opt. Spectrosc</addtitle><description>A new spectrofluorometric method for the determination of glutathione based on the reaction catalyzed by hemoglobin was reported. The reaction product gave a highly fluorescent intensity with the excitation and emission wavelengths of 320.0 nm and 413.0 nm, respectively. The optimum experimental conditions were investigated. Results showed that low concentration glutathione enhanced the fluorescence intensity significantly. The line ranges were 1.0 × 10
–6
–1.0 × 10
–5
mol L
–1
of glutathione and 6.0 × 10
-10
mol L
-1
–1.0 × 10
-8
mol L
-1
, respectively. The detection limit was calculated to be 1.1 × 10
-11
mol L
-1
. The recovery test by the standard addition method gave values in the range of 90.78%-102.20%. This method was used for the determination of glutathione in synthetic and real samples with satisfactory results.</description><subject>Geometrical and Applied Optics</subject><subject>Lasers</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Photonics</subject><subject>Physics</subject><subject>Physics and Astronomy</subject><issn>0030-400X</issn><issn>1562-6911</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp9kEFLxDAQhYMoWFd_gLf-gepM0tTkKIu6wooHFbyVNJ1su7SNpNnD-uvNst4ET4-ZN2_4eIxdI9wgivL2DUBACfCJEjlwVCcsQ1nxotKIpyw72MXBP2cX87wFQFSlztjLika_GXzTT4U10Qz7b2pzN-x88CPF0Ns8SefTzoc8dpS3FCmM_WRi76fcu3wz7KKJXZrokp05M8x09asL9vH48L5cFevXp-fl_bqwXKlYtNA2QpHT0londMIUUBHHtrVGVbyRjXEgudLyTpQCAUupgPNSkwad0MWC4fGvDX6eA7n6K_SjCfsaoT70Uf_pI2X4MTOn22lDod76XZgS5j-hH-L_Yks</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>Wang, Ruiqiang</creator><creator>Tang, Lin</creator><creator>Li, Hua</creator><creator>Wang, Yi</creator><creator>Gou, Rong</creator><creator>Guo, Yuanyuan</creator><creator>Fang, Yudong</creator><creator>Chen, Fengmei</creator><general>Pleiades Publishing</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2016</creationdate><title>Hemoglobin-catalyzed fluorometric method for the determination of glutathione</title><author>Wang, Ruiqiang ; Tang, Lin ; Li, Hua ; Wang, Yi ; Gou, Rong ; Guo, Yuanyuan ; Fang, Yudong ; Chen, Fengmei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c288t-d0db38ef95ccf39562306e21ddca862b5baf052895734310145802249e9090013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Geometrical and Applied Optics</topic><topic>Lasers</topic><topic>Optical Devices</topic><topic>Optics</topic><topic>Photonics</topic><topic>Physics</topic><topic>Physics and Astronomy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Ruiqiang</creatorcontrib><creatorcontrib>Tang, Lin</creatorcontrib><creatorcontrib>Li, Hua</creatorcontrib><creatorcontrib>Wang, Yi</creatorcontrib><creatorcontrib>Gou, Rong</creatorcontrib><creatorcontrib>Guo, Yuanyuan</creatorcontrib><creatorcontrib>Fang, Yudong</creatorcontrib><creatorcontrib>Chen, Fengmei</creatorcontrib><collection>CrossRef</collection><jtitle>Optics and spectroscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Ruiqiang</au><au>Tang, Lin</au><au>Li, Hua</au><au>Wang, Yi</au><au>Gou, Rong</au><au>Guo, Yuanyuan</au><au>Fang, Yudong</au><au>Chen, Fengmei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hemoglobin-catalyzed fluorometric method for the determination of glutathione</atitle><jtitle>Optics and spectroscopy</jtitle><stitle>Opt. Spectrosc</stitle><date>2016</date><risdate>2016</risdate><volume>120</volume><issue>1</issue><spage>164</spage><epage>170</epage><pages>164-170</pages><issn>0030-400X</issn><eissn>1562-6911</eissn><abstract>A new spectrofluorometric method for the determination of glutathione based on the reaction catalyzed by hemoglobin was reported. The reaction product gave a highly fluorescent intensity with the excitation and emission wavelengths of 320.0 nm and 413.0 nm, respectively. The optimum experimental conditions were investigated. Results showed that low concentration glutathione enhanced the fluorescence intensity significantly. The line ranges were 1.0 × 10
–6
–1.0 × 10
–5
mol L
–1
of glutathione and 6.0 × 10
-10
mol L
-1
–1.0 × 10
-8
mol L
-1
, respectively. The detection limit was calculated to be 1.1 × 10
-11
mol L
-1
. The recovery test by the standard addition method gave values in the range of 90.78%-102.20%. This method was used for the determination of glutathione in synthetic and real samples with satisfactory results.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0030400X15120218</doi><tpages>7</tpages></addata></record> |
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| ispartof | Optics and spectroscopy, 2016, Vol.120 (1), p.164-170 |
| issn | 0030-400X 1562-6911 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Geometrical and Applied Optics Lasers Optical Devices Optics Photonics Physics Physics and Astronomy |
| title | Hemoglobin-catalyzed fluorometric method for the determination of glutathione |
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