Coexistence of a nonresistance-conferring IncI1 plasmid favors persistence of the bla CTX-M -bearing IncFII plasmid in Escherichia coli
The interaction between coexisting plasmids can affect plasmid-carried resistance gene persistence and spread. However, whether the persistence of the gene in clinical Enterobacteriaceae is related to the interaction of coresident nonresistance-conferring plasmids has not been reported. This study w...
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Veröffentlicht in: | Microbiology spectrum 2024-06, Vol.12 (6), p.e0424023 |
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Zusammenfassung: | The interaction between coexisting plasmids can affect plasmid-carried resistance gene persistence and spread. However, whether the persistence of the
gene in clinical Enterobacteriaceae is related to the interaction of coresident nonresistance-conferring plasmids has not been reported. This study was initiated to elucidate how a nonresistance-conferring IncI1 plasmid affected the
-bearing IncFII plasmid colocated on the same cell. Herein, we constructed three isogenic derivatives of
C600, designated as C600
, C600
, and C600
, which harbored the
-IncFII plasmid and/or the nonresistance-IncI1 one. We discovered that strain C600
conferred higher fitness advantages than strain C600
; also, the stability of the
-IncFII plasmid was noticeably improved in an antibiotic-free environment when it coexisted with the IncI1 plasmid. To further explore why the IncI1 plasmid enhanced the persistence of the
-IncFII plasmid, we assessed the
-IncFII plasmid's copy numbers, conjugation frequencies, and
gene expressions in strains C600
and C600
. The results demonstrated that the
expressions of the
-IncFII plasmid in strain C600
was greatly decreased, along with the plasmid's copy numbers and mating efficiencies, compared to those in strain C600
. Moreover, further study revealed that the intracellular ATP levels of strain C600
were far lower than those of strain C600
. Our findings confirmed that coexistence of the nonresistance-IncI1 plasmid can keep the
-IncFII plasmid more stable by increasing the fitness advantages of the host bacteria, which will pose a threat to preventing the long-term presence of the plasmid-carried
gene in clinical Enterobacteriaceae.
So far, plasmid-carried
is still the most common extended-spectrum beta-lactamase (ESBL) genotype in clinical settings worldwide. Except for the widespread use of third-generation cephalosporins, the interaction between coexisting plasmids can also affect the long-term stable existence of the
gene; however, the study on that is still sparse. In the present study, we assess the interaction of coinhabitant plasmids
-IncFII and nonresistance-IncI1. Our results confirmed that the increased fitness advantages of strain C600
were attributable to the cohabitant nonresistance-IncI1 plasmid, which largely reduced the intracellular ATP levels of host bacteria, thus decreasing the rep gene expression of the
-IncFII plasmid, its copy numbers, and mating efficiencies, while the higher fitness advantages of strain C600
enhanced th |
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ISSN: | 2165-0497 2165-0497 |
DOI: | 10.1128/spectrum.04240-23 |