Evaluation of bla OXA-48-like point mutation carbapenemase-producing Enterobacterales in Prapokklao Hospital, Thailand
Carbapenemase-producing (CPE) isolates increasingly carry oxacillinase-48 (OXA-48)-like enzymes encoded by , which can confer high levels of carbapenem resistance. This aims to determine the prevalence of CPE and genetic variation among -carrying isolates recovered from Prapokklao Hospital in Chanth...
Gespeichert in:
| Veröffentlicht in: | Microbiology spectrum 2024-12, Vol.12 (12), p.e0019824 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 12 |
| container_start_page | e0019824 |
| container_title | Microbiology spectrum |
| container_volume | 12 |
| creator | Santajit, Sirijan Tunyong, Witawat Kong-Ngoen, Thida Arsheewa, Weewan Hinthong, Woranich Pumirat, Pornpan Sookrung, Nitat Indrawattana, Nitaya |
| description | Carbapenemase-producing
(CPE) isolates increasingly carry oxacillinase-48 (OXA-48)-like enzymes encoded by
, which can confer high levels of carbapenem resistance. This aims to determine the prevalence of CPE and genetic variation among
-carrying isolates recovered from Prapokklao Hospital in Chanthaburi Province, Thailand in 2016-2017. In total, 122 carbapenem-resistant
(CRE) isolates were recovered from clinical samples. CRE were evaluated using standard biochemical tests and MIC test strips. Carbapenemase production was assessed through the modified Hodge test (MHT), modified carbapenem inactivation method (mCIM), and EDTA-modified carbapenem inactivation method (eCIM). Detection of
mutations was conducted via PCR and confirmed by Sanger sequencing. Among these CRE isolates, 72 (59.02%), 44 (36.07%), 3 (2.46%), and 3 (2.46%) were
,
,
, and
, respectively. The MHT identified carbapenemase production in 108 isolates (88.52%). Based on the mCIM, 81 isolates (66.39%) were carbapenemase producers. Seventy-three isolates (59.84%) were eCIM-positive, indicating metallo-β-lactamase production. Three distinct genetic variants of the
gene were identified among the isolates, including the wild-type and two point mutation types harboring the mutations E168Q and S171A (mutation type 1) and E168Q, S171A, and R214S (mutation type 2). Multiple-sequence alignment and
analysis revealed variation of R214 located in the β5-β6 loop. This study identified
point mutation groups and carbapenemase production, predominantly metallo-β-lactamases, among CRE isolates, especially
and
. These findings highlight the importance of implementing stringent infection control measures and active antimicrobial resistance surveillance to combat the spread of difficult-to-treat, metallo-β-lactamase-producing CRE in healthcare settings.
In this study, we aimed to investigate genetic variation and CPE among bla
carrying isolates recovered from Prapokklao Hospital, Chanthaburi Province, Thailand, during 2016-2017. A total of 122 carbapenem-resistant Enterobacterales (CRE) were recovered from clinical samples in Prapokklao Hospital. All CRE samples were confirmed by standard biochemical tests and minimum inhibitory concentration (MIC) test strips (E-test). The carbapenemase production was determined using the modified Hodge test (MHT), the modified carbapenem inactivation method (mCIM), and EDTA-CIM (eCIM). Three single mutations (E168Q, S171A, and R214S) were characterized in this study. This mut |
| doi_str_mv | 10.1128/spectrum.00198-24 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1128_spectrum_00198_24</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>39417625</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1084-9f459fde877a2c97b53cd04db6d9737557e6250df2facdb2ce4ccf5bd65abd713</originalsourceid><addsrcrecordid>eNpNkF1LwzAUhoMoTuZ-gDeSH2BmkiZNeznGdMJgXkzwrpx8VOvapiTtwH9vdU68es_F-7xwHoRuGJ0zxrP72DnTh6GZU8ryjHBxhq44SyWhIlfn_-4JmsX4Qccao5JLfokmSS6YSrm8QofVAeoB-sq32JdY14C3rwsiMlJXe4c7X7U9bob-2DAQNHSudQ1ER7rg7WCq9g2v2t4Fr8GMAbWLuGrxc4DO7_c1eLz2sat6qO_w7h2qGlp7jS5KqKOb_eYUvTysdss12Wwfn5aLDTGMZoLkpZB5aV2mFHCTKy0TY6mwOrW5SpSUyo1fUFvyEozV3DhhTCm1TSVoq1gyRey4a4KPMbiy6ELVQPgsGC2-NRYnjcWPxoKLkbk9Mt2gG2f_iJO05AvxonLv</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Evaluation of bla OXA-48-like point mutation carbapenemase-producing Enterobacterales in Prapokklao Hospital, Thailand</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Santajit, Sirijan ; Tunyong, Witawat ; Kong-Ngoen, Thida ; Arsheewa, Weewan ; Hinthong, Woranich ; Pumirat, Pornpan ; Sookrung, Nitat ; Indrawattana, Nitaya</creator><contributor>Sekyere, John Osei</contributor><creatorcontrib>Santajit, Sirijan ; Tunyong, Witawat ; Kong-Ngoen, Thida ; Arsheewa, Weewan ; Hinthong, Woranich ; Pumirat, Pornpan ; Sookrung, Nitat ; Indrawattana, Nitaya ; Sekyere, John Osei</creatorcontrib><description>Carbapenemase-producing
(CPE) isolates increasingly carry oxacillinase-48 (OXA-48)-like enzymes encoded by
, which can confer high levels of carbapenem resistance. This aims to determine the prevalence of CPE and genetic variation among
-carrying isolates recovered from Prapokklao Hospital in Chanthaburi Province, Thailand in 2016-2017. In total, 122 carbapenem-resistant
(CRE) isolates were recovered from clinical samples. CRE were evaluated using standard biochemical tests and MIC test strips. Carbapenemase production was assessed through the modified Hodge test (MHT), modified carbapenem inactivation method (mCIM), and EDTA-modified carbapenem inactivation method (eCIM). Detection of
mutations was conducted via PCR and confirmed by Sanger sequencing. Among these CRE isolates, 72 (59.02%), 44 (36.07%), 3 (2.46%), and 3 (2.46%) were
,
,
, and
, respectively. The MHT identified carbapenemase production in 108 isolates (88.52%). Based on the mCIM, 81 isolates (66.39%) were carbapenemase producers. Seventy-three isolates (59.84%) were eCIM-positive, indicating metallo-β-lactamase production. Three distinct genetic variants of the
gene were identified among the isolates, including the wild-type and two point mutation types harboring the mutations E168Q and S171A (mutation type 1) and E168Q, S171A, and R214S (mutation type 2). Multiple-sequence alignment and
analysis revealed variation of R214 located in the β5-β6 loop. This study identified
point mutation groups and carbapenemase production, predominantly metallo-β-lactamases, among CRE isolates, especially
and
. These findings highlight the importance of implementing stringent infection control measures and active antimicrobial resistance surveillance to combat the spread of difficult-to-treat, metallo-β-lactamase-producing CRE in healthcare settings.
In this study, we aimed to investigate genetic variation and CPE among bla
carrying isolates recovered from Prapokklao Hospital, Chanthaburi Province, Thailand, during 2016-2017. A total of 122 carbapenem-resistant Enterobacterales (CRE) were recovered from clinical samples in Prapokklao Hospital. All CRE samples were confirmed by standard biochemical tests and minimum inhibitory concentration (MIC) test strips (E-test). The carbapenemase production was determined using the modified Hodge test (MHT), the modified carbapenem inactivation method (mCIM), and EDTA-CIM (eCIM). Three single mutations (E168Q, S171A, and R214S) were characterized in this study. This mutation might reflect the hydrolysis of the modified β-lactam spectrum, especially carbapenem, by OXA-48-like. Our report provides evidence of the bla
point mutation and carbapenemase-producing phenotype of CRE detected in this healthcare setting. Effective control measures and active surveillance of drug resistance in nosocomial pathogens are crucial for controlling diseases associated with difficult-to-treat bacteria.</description><identifier>ISSN: 2165-0497</identifier><identifier>EISSN: 2165-0497</identifier><identifier>DOI: 10.1128/spectrum.00198-24</identifier><identifier>PMID: 39417625</identifier><language>eng</language><publisher>United States</publisher><ispartof>Microbiology spectrum, 2024-12, Vol.12 (12), p.e0019824</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1084-9f459fde877a2c97b53cd04db6d9737557e6250df2facdb2ce4ccf5bd65abd713</cites><orcidid>0000-0002-1273-2618 ; 0000-0003-1571-528X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39417625$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sekyere, John Osei</contributor><creatorcontrib>Santajit, Sirijan</creatorcontrib><creatorcontrib>Tunyong, Witawat</creatorcontrib><creatorcontrib>Kong-Ngoen, Thida</creatorcontrib><creatorcontrib>Arsheewa, Weewan</creatorcontrib><creatorcontrib>Hinthong, Woranich</creatorcontrib><creatorcontrib>Pumirat, Pornpan</creatorcontrib><creatorcontrib>Sookrung, Nitat</creatorcontrib><creatorcontrib>Indrawattana, Nitaya</creatorcontrib><title>Evaluation of bla OXA-48-like point mutation carbapenemase-producing Enterobacterales in Prapokklao Hospital, Thailand</title><title>Microbiology spectrum</title><addtitle>Microbiol Spectr</addtitle><description>Carbapenemase-producing
(CPE) isolates increasingly carry oxacillinase-48 (OXA-48)-like enzymes encoded by
, which can confer high levels of carbapenem resistance. This aims to determine the prevalence of CPE and genetic variation among
-carrying isolates recovered from Prapokklao Hospital in Chanthaburi Province, Thailand in 2016-2017. In total, 122 carbapenem-resistant
(CRE) isolates were recovered from clinical samples. CRE were evaluated using standard biochemical tests and MIC test strips. Carbapenemase production was assessed through the modified Hodge test (MHT), modified carbapenem inactivation method (mCIM), and EDTA-modified carbapenem inactivation method (eCIM). Detection of
mutations was conducted via PCR and confirmed by Sanger sequencing. Among these CRE isolates, 72 (59.02%), 44 (36.07%), 3 (2.46%), and 3 (2.46%) were
,
,
, and
, respectively. The MHT identified carbapenemase production in 108 isolates (88.52%). Based on the mCIM, 81 isolates (66.39%) were carbapenemase producers. Seventy-three isolates (59.84%) were eCIM-positive, indicating metallo-β-lactamase production. Three distinct genetic variants of the
gene were identified among the isolates, including the wild-type and two point mutation types harboring the mutations E168Q and S171A (mutation type 1) and E168Q, S171A, and R214S (mutation type 2). Multiple-sequence alignment and
analysis revealed variation of R214 located in the β5-β6 loop. This study identified
point mutation groups and carbapenemase production, predominantly metallo-β-lactamases, among CRE isolates, especially
and
. These findings highlight the importance of implementing stringent infection control measures and active antimicrobial resistance surveillance to combat the spread of difficult-to-treat, metallo-β-lactamase-producing CRE in healthcare settings.
In this study, we aimed to investigate genetic variation and CPE among bla
carrying isolates recovered from Prapokklao Hospital, Chanthaburi Province, Thailand, during 2016-2017. A total of 122 carbapenem-resistant Enterobacterales (CRE) were recovered from clinical samples in Prapokklao Hospital. All CRE samples were confirmed by standard biochemical tests and minimum inhibitory concentration (MIC) test strips (E-test). The carbapenemase production was determined using the modified Hodge test (MHT), the modified carbapenem inactivation method (mCIM), and EDTA-CIM (eCIM). Three single mutations (E168Q, S171A, and R214S) were characterized in this study. This mutation might reflect the hydrolysis of the modified β-lactam spectrum, especially carbapenem, by OXA-48-like. Our report provides evidence of the bla
point mutation and carbapenemase-producing phenotype of CRE detected in this healthcare setting. Effective control measures and active surveillance of drug resistance in nosocomial pathogens are crucial for controlling diseases associated with difficult-to-treat bacteria.</description><issn>2165-0497</issn><issn>2165-0497</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNpNkF1LwzAUhoMoTuZ-gDeSH2BmkiZNeznGdMJgXkzwrpx8VOvapiTtwH9vdU68es_F-7xwHoRuGJ0zxrP72DnTh6GZU8ryjHBxhq44SyWhIlfn_-4JmsX4Qccao5JLfokmSS6YSrm8QofVAeoB-sq32JdY14C3rwsiMlJXe4c7X7U9bob-2DAQNHSudQ1ER7rg7WCq9g2v2t4Fr8GMAbWLuGrxc4DO7_c1eLz2sat6qO_w7h2qGlp7jS5KqKOb_eYUvTysdss12Wwfn5aLDTGMZoLkpZB5aV2mFHCTKy0TY6mwOrW5SpSUyo1fUFvyEozV3DhhTCm1TSVoq1gyRey4a4KPMbiy6ELVQPgsGC2-NRYnjcWPxoKLkbk9Mt2gG2f_iJO05AvxonLv</recordid><startdate>20241205</startdate><enddate>20241205</enddate><creator>Santajit, Sirijan</creator><creator>Tunyong, Witawat</creator><creator>Kong-Ngoen, Thida</creator><creator>Arsheewa, Weewan</creator><creator>Hinthong, Woranich</creator><creator>Pumirat, Pornpan</creator><creator>Sookrung, Nitat</creator><creator>Indrawattana, Nitaya</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-1273-2618</orcidid><orcidid>https://orcid.org/0000-0003-1571-528X</orcidid></search><sort><creationdate>20241205</creationdate><title>Evaluation of bla OXA-48-like point mutation carbapenemase-producing Enterobacterales in Prapokklao Hospital, Thailand</title><author>Santajit, Sirijan ; Tunyong, Witawat ; Kong-Ngoen, Thida ; Arsheewa, Weewan ; Hinthong, Woranich ; Pumirat, Pornpan ; Sookrung, Nitat ; Indrawattana, Nitaya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1084-9f459fde877a2c97b53cd04db6d9737557e6250df2facdb2ce4ccf5bd65abd713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Santajit, Sirijan</creatorcontrib><creatorcontrib>Tunyong, Witawat</creatorcontrib><creatorcontrib>Kong-Ngoen, Thida</creatorcontrib><creatorcontrib>Arsheewa, Weewan</creatorcontrib><creatorcontrib>Hinthong, Woranich</creatorcontrib><creatorcontrib>Pumirat, Pornpan</creatorcontrib><creatorcontrib>Sookrung, Nitat</creatorcontrib><creatorcontrib>Indrawattana, Nitaya</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Microbiology spectrum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Santajit, Sirijan</au><au>Tunyong, Witawat</au><au>Kong-Ngoen, Thida</au><au>Arsheewa, Weewan</au><au>Hinthong, Woranich</au><au>Pumirat, Pornpan</au><au>Sookrung, Nitat</au><au>Indrawattana, Nitaya</au><au>Sekyere, John Osei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of bla OXA-48-like point mutation carbapenemase-producing Enterobacterales in Prapokklao Hospital, Thailand</atitle><jtitle>Microbiology spectrum</jtitle><addtitle>Microbiol Spectr</addtitle><date>2024-12-05</date><risdate>2024</risdate><volume>12</volume><issue>12</issue><spage>e0019824</spage><pages>e0019824-</pages><issn>2165-0497</issn><eissn>2165-0497</eissn><abstract>Carbapenemase-producing
(CPE) isolates increasingly carry oxacillinase-48 (OXA-48)-like enzymes encoded by
, which can confer high levels of carbapenem resistance. This aims to determine the prevalence of CPE and genetic variation among
-carrying isolates recovered from Prapokklao Hospital in Chanthaburi Province, Thailand in 2016-2017. In total, 122 carbapenem-resistant
(CRE) isolates were recovered from clinical samples. CRE were evaluated using standard biochemical tests and MIC test strips. Carbapenemase production was assessed through the modified Hodge test (MHT), modified carbapenem inactivation method (mCIM), and EDTA-modified carbapenem inactivation method (eCIM). Detection of
mutations was conducted via PCR and confirmed by Sanger sequencing. Among these CRE isolates, 72 (59.02%), 44 (36.07%), 3 (2.46%), and 3 (2.46%) were
,
,
, and
, respectively. The MHT identified carbapenemase production in 108 isolates (88.52%). Based on the mCIM, 81 isolates (66.39%) were carbapenemase producers. Seventy-three isolates (59.84%) were eCIM-positive, indicating metallo-β-lactamase production. Three distinct genetic variants of the
gene were identified among the isolates, including the wild-type and two point mutation types harboring the mutations E168Q and S171A (mutation type 1) and E168Q, S171A, and R214S (mutation type 2). Multiple-sequence alignment and
analysis revealed variation of R214 located in the β5-β6 loop. This study identified
point mutation groups and carbapenemase production, predominantly metallo-β-lactamases, among CRE isolates, especially
and
. These findings highlight the importance of implementing stringent infection control measures and active antimicrobial resistance surveillance to combat the spread of difficult-to-treat, metallo-β-lactamase-producing CRE in healthcare settings.
In this study, we aimed to investigate genetic variation and CPE among bla
carrying isolates recovered from Prapokklao Hospital, Chanthaburi Province, Thailand, during 2016-2017. A total of 122 carbapenem-resistant Enterobacterales (CRE) were recovered from clinical samples in Prapokklao Hospital. All CRE samples were confirmed by standard biochemical tests and minimum inhibitory concentration (MIC) test strips (E-test). The carbapenemase production was determined using the modified Hodge test (MHT), the modified carbapenem inactivation method (mCIM), and EDTA-CIM (eCIM). Three single mutations (E168Q, S171A, and R214S) were characterized in this study. This mutation might reflect the hydrolysis of the modified β-lactam spectrum, especially carbapenem, by OXA-48-like. Our report provides evidence of the bla
point mutation and carbapenemase-producing phenotype of CRE detected in this healthcare setting. Effective control measures and active surveillance of drug resistance in nosocomial pathogens are crucial for controlling diseases associated with difficult-to-treat bacteria.</abstract><cop>United States</cop><pmid>39417625</pmid><doi>10.1128/spectrum.00198-24</doi><orcidid>https://orcid.org/0000-0002-1273-2618</orcidid><orcidid>https://orcid.org/0000-0003-1571-528X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2165-0497 |
| ispartof | Microbiology spectrum, 2024-12, Vol.12 (12), p.e0019824 |
| issn | 2165-0497 2165-0497 |
| language | eng |
| recordid | cdi_crossref_primary_10_1128_spectrum_00198_24 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| title | Evaluation of bla OXA-48-like point mutation carbapenemase-producing Enterobacterales in Prapokklao Hospital, Thailand |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T15%3A52%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20bla%20OXA-48-like%20point%20mutation%20carbapenemase-producing%20Enterobacterales%20in%20Prapokklao%20Hospital,%20Thailand&rft.jtitle=Microbiology%20spectrum&rft.au=Santajit,%20Sirijan&rft.date=2024-12-05&rft.volume=12&rft.issue=12&rft.spage=e0019824&rft.pages=e0019824-&rft.issn=2165-0497&rft.eissn=2165-0497&rft_id=info:doi/10.1128/spectrum.00198-24&rft_dat=%3Cpubmed_cross%3E39417625%3C/pubmed_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/39417625&rfr_iscdi=true |