emm1/Sequence Type 28 Strains of Group A Streptococci That Express covR at Early Stationary Phase Are Associated with Increased Growth and Earlier SpeB Secretion
Streptococcus pyogenes (group A streptococcus [GAS]) is a versatile human pathogen, and emm1/sequence type 28 (ST28) is the most frequently isolated type from GAS infections. The emm1/ST28 strain is associated with necrotizing fasciitis and streptococcal toxic shock syndrome. Growth-phase regulation...
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| Veröffentlicht in: | Journal of Clinical Microbiology 2009-10, Vol.47 (10), p.3161-3169 |
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| creator | Chiang-Ni, Chuan Zheng, Po-Xing Ho, Yueh-Ren Wu, Hsiu-Mei Chuang, Woei-Jer Lin, Yee-Shin Lin, Ming-T Liu, Ching-Chuan Wu, Jiunn-Jong |
| description | Streptococcus pyogenes (group A streptococcus [GAS]) is a versatile human pathogen, and emm1/sequence type 28 (ST28) is the most frequently isolated type from GAS infections. The emm1/ST28 strain is associated with necrotizing fasciitis and streptococcal toxic shock syndrome. Growth-phase regulation is one of the important regulatory mechanisms in GAS, which controls gene expression at restricted phases of growth. CovRS, a two-component regulatory system, is considered the regulator of streptococcal pyrogenic exotoxin B (SpeB) and is thought to be activated in the exponential phase of growth. In the present study, Northern hybridization analysis showed that 52% of the analyzed GAS strains expressed covR at the exponential phase, but 48% of the strains expressed covR at the early stationary phase of growth. Strains transcribing covR at the early stationary phase showed better growth and earlier SpeB expression than the other group of strains. Multilocus sequence typing and pulsed-field gel electrophoresis analysis showed only emm1/ST28 strains (which comprise a clonal cluster) were expressing covR at the early stationary phase of growth, indicating that emm1/ST28 strains have special characteristics which may be related to their worldwide distribution. |
| doi_str_mv | 10.1128/JCM.00202-09 |
| format | Article |
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The emm1/ST28 strain is associated with necrotizing fasciitis and streptococcal toxic shock syndrome. Growth-phase regulation is one of the important regulatory mechanisms in GAS, which controls gene expression at restricted phases of growth. CovRS, a two-component regulatory system, is considered the regulator of streptococcal pyrogenic exotoxin B (SpeB) and is thought to be activated in the exponential phase of growth. In the present study, Northern hybridization analysis showed that 52% of the analyzed GAS strains expressed covR at the exponential phase, but 48% of the strains expressed covR at the early stationary phase of growth. Strains transcribing covR at the early stationary phase showed better growth and earlier SpeB expression than the other group of strains. Multilocus sequence typing and pulsed-field gel electrophoresis analysis showed only emm1/ST28 strains (which comprise a clonal cluster) were expressing covR at the early stationary phase of growth, indicating that emm1/ST28 strains have special characteristics which may be related to their worldwide distribution.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.00202-09</identifier><identifier>PMID: 19710271</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Antigens, Bacterial - genetics ; Bacterial Outer Membrane Proteins - genetics ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - metabolism ; Bacteriology ; Biological and medical sciences ; Blotting, Northern ; Carrier Proteins - genetics ; Cluster Analysis ; DNA Fingerprinting ; DNA, Bacterial - genetics ; Electrophoresis, Gel, Pulsed-Field ; Exotoxins - biosynthesis ; Fundamental and applied biological sciences. Psychology ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial ; Genotype ; Humans ; Microbiology ; Miscellaneous ; Repressor Proteins - metabolism ; Sequence Analysis, DNA ; Signal Transduction ; Streptococcal Infections - microbiology ; Streptococcus pyogenes ; Streptococcus pyogenes - classification ; Streptococcus pyogenes - genetics ; Streptococcus pyogenes - isolation & purification ; Streptococcus pyogenes - physiology</subject><ispartof>Journal of Clinical Microbiology, 2009-10, Vol.47 (10), p.3161-3169</ispartof><rights>2009 INIST-CNRS</rights><rights>Copyright © 2009, American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-17bb725f1cba3a1e5938d502d72dfcbe835182d6d8eb277ec9b25543402db74c3</citedby><cites>FETCH-LOGICAL-c495t-17bb725f1cba3a1e5938d502d72dfcbe835182d6d8eb277ec9b25543402db74c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756925/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756925/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21997266$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19710271$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chiang-Ni, Chuan</creatorcontrib><creatorcontrib>Zheng, Po-Xing</creatorcontrib><creatorcontrib>Ho, Yueh-Ren</creatorcontrib><creatorcontrib>Wu, Hsiu-Mei</creatorcontrib><creatorcontrib>Chuang, Woei-Jer</creatorcontrib><creatorcontrib>Lin, Yee-Shin</creatorcontrib><creatorcontrib>Lin, Ming-T</creatorcontrib><creatorcontrib>Liu, Ching-Chuan</creatorcontrib><creatorcontrib>Wu, Jiunn-Jong</creatorcontrib><title>emm1/Sequence Type 28 Strains of Group A Streptococci That Express covR at Early Stationary Phase Are Associated with Increased Growth and Earlier SpeB Secretion</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>Streptococcus pyogenes (group A streptococcus [GAS]) is a versatile human pathogen, and emm1/sequence type 28 (ST28) is the most frequently isolated type from GAS infections. The emm1/ST28 strain is associated with necrotizing fasciitis and streptococcal toxic shock syndrome. Growth-phase regulation is one of the important regulatory mechanisms in GAS, which controls gene expression at restricted phases of growth. CovRS, a two-component regulatory system, is considered the regulator of streptococcal pyrogenic exotoxin B (SpeB) and is thought to be activated in the exponential phase of growth. In the present study, Northern hybridization analysis showed that 52% of the analyzed GAS strains expressed covR at the exponential phase, but 48% of the strains expressed covR at the early stationary phase of growth. Strains transcribing covR at the early stationary phase showed better growth and earlier SpeB expression than the other group of strains. Multilocus sequence typing and pulsed-field gel electrophoresis analysis showed only emm1/ST28 strains (which comprise a clonal cluster) were expressing covR at the early stationary phase of growth, indicating that emm1/ST28 strains have special characteristics which may be related to their worldwide distribution.</description><subject>Antigens, Bacterial - genetics</subject><subject>Bacterial Outer Membrane Proteins - genetics</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Carrier Proteins - genetics</subject><subject>Cluster Analysis</subject><subject>DNA Fingerprinting</subject><subject>DNA, Bacterial - genetics</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Exotoxins - biosynthesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genotype</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Repressor Proteins - metabolism</subject><subject>Sequence Analysis, DNA</subject><subject>Signal Transduction</subject><subject>Streptococcal Infections - microbiology</subject><subject>Streptococcus pyogenes</subject><subject>Streptococcus pyogenes - classification</subject><subject>Streptococcus pyogenes - genetics</subject><subject>Streptococcus pyogenes - isolation & purification</subject><subject>Streptococcus pyogenes - physiology</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUFv0zAUxyMEYmNw4wwWEpzI5ufEcXyZVKoxhoZAtJO4WY7z0nhq4mCnK_04fFOctRpwsCz7_fyz_f5J8hLoKQArzz7Pv5xSyihLqXyUHAOVZVoU9Mfj5JhSyVOATBwlz0K4pRTynPOnyRFIAZQJOE5-Y9fB2QJ_brA3SJa7AQkryWL02vaBuIZcercZyGzawmF0xhljybLVI7n4NXgMgRh3951Ma-3Xu8jp0bpe-x351uqAZObjCMEZq0esydaOLbnqjcdYrCf9Nm7ovr4_b9GTxYAfyAIjMYmeJ08avQ744jCfJDcfL5bzT-n118ur-ew6NbnkYwqiqgTjDZhKZxqQy6ysOWW1YHVjKiwzDiWri7rEigmBRlaM8zzLI1KJ3GQnyfneO2yqDmuDfezBWg3edvEvymmr_q_0tlUrd6eY4IVkPAreHQTexXaGUXU2GFyvdY9uExSLaYGECXy_B413IXhsHi4BqqZMVcxU3WeqqIz4q38f9hc-hBiBtwdAB6PXjde9seGBYyClYEURuTd7rrWrdms9Kh06dWs6lYvp6gyKSfZ6DzXaKb3yUXSzYBQyCkVZZFJmfwDYmsA0</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>Chiang-Ni, Chuan</creator><creator>Zheng, Po-Xing</creator><creator>Ho, Yueh-Ren</creator><creator>Wu, Hsiu-Mei</creator><creator>Chuang, Woei-Jer</creator><creator>Lin, Yee-Shin</creator><creator>Lin, Ming-T</creator><creator>Liu, Ching-Chuan</creator><creator>Wu, Jiunn-Jong</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>5PM</scope></search><sort><creationdate>20091001</creationdate><title>emm1/Sequence Type 28 Strains of Group A Streptococci That Express covR at Early Stationary Phase Are Associated with Increased Growth and Earlier SpeB Secretion</title><author>Chiang-Ni, Chuan ; Zheng, Po-Xing ; Ho, Yueh-Ren ; Wu, Hsiu-Mei ; Chuang, Woei-Jer ; Lin, Yee-Shin ; Lin, Ming-T ; Liu, Ching-Chuan ; Wu, Jiunn-Jong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-17bb725f1cba3a1e5938d502d72dfcbe835182d6d8eb277ec9b25543402db74c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Antigens, Bacterial - genetics</topic><topic>Bacterial Outer Membrane Proteins - genetics</topic><topic>Bacterial Proteins - biosynthesis</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Carrier Proteins - genetics</topic><topic>Cluster Analysis</topic><topic>DNA Fingerprinting</topic><topic>DNA, Bacterial - genetics</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Exotoxins - biosynthesis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genotype</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Repressor Proteins - metabolism</topic><topic>Sequence Analysis, DNA</topic><topic>Signal Transduction</topic><topic>Streptococcal Infections - microbiology</topic><topic>Streptococcus pyogenes</topic><topic>Streptococcus pyogenes - classification</topic><topic>Streptococcus pyogenes - genetics</topic><topic>Streptococcus pyogenes - isolation & purification</topic><topic>Streptococcus pyogenes - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chiang-Ni, Chuan</creatorcontrib><creatorcontrib>Zheng, Po-Xing</creatorcontrib><creatorcontrib>Ho, Yueh-Ren</creatorcontrib><creatorcontrib>Wu, Hsiu-Mei</creatorcontrib><creatorcontrib>Chuang, Woei-Jer</creatorcontrib><creatorcontrib>Lin, Yee-Shin</creatorcontrib><creatorcontrib>Lin, Ming-T</creatorcontrib><creatorcontrib>Liu, Ching-Chuan</creatorcontrib><creatorcontrib>Wu, Jiunn-Jong</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chiang-Ni, Chuan</au><au>Zheng, Po-Xing</au><au>Ho, Yueh-Ren</au><au>Wu, Hsiu-Mei</au><au>Chuang, Woei-Jer</au><au>Lin, Yee-Shin</au><au>Lin, Ming-T</au><au>Liu, Ching-Chuan</au><au>Wu, Jiunn-Jong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>emm1/Sequence Type 28 Strains of Group A Streptococci That Express covR at Early Stationary Phase Are Associated with Increased Growth and Earlier SpeB Secretion</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>47</volume><issue>10</issue><spage>3161</spage><epage>3169</epage><pages>3161-3169</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><coden>JCMIDW</coden><abstract>Streptococcus pyogenes (group A streptococcus [GAS]) is a versatile human pathogen, and emm1/sequence type 28 (ST28) is the most frequently isolated type from GAS infections. The emm1/ST28 strain is associated with necrotizing fasciitis and streptococcal toxic shock syndrome. Growth-phase regulation is one of the important regulatory mechanisms in GAS, which controls gene expression at restricted phases of growth. CovRS, a two-component regulatory system, is considered the regulator of streptococcal pyrogenic exotoxin B (SpeB) and is thought to be activated in the exponential phase of growth. In the present study, Northern hybridization analysis showed that 52% of the analyzed GAS strains expressed covR at the exponential phase, but 48% of the strains expressed covR at the early stationary phase of growth. Strains transcribing covR at the early stationary phase showed better growth and earlier SpeB expression than the other group of strains. Multilocus sequence typing and pulsed-field gel electrophoresis analysis showed only emm1/ST28 strains (which comprise a clonal cluster) were expressing covR at the early stationary phase of growth, indicating that emm1/ST28 strains have special characteristics which may be related to their worldwide distribution.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>19710271</pmid><doi>10.1128/JCM.00202-09</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antigens, Bacterial - genetics Bacterial Outer Membrane Proteins - genetics Bacterial Proteins - biosynthesis Bacterial Proteins - metabolism Bacteriology Biological and medical sciences Blotting, Northern Carrier Proteins - genetics Cluster Analysis DNA Fingerprinting DNA, Bacterial - genetics Electrophoresis, Gel, Pulsed-Field Exotoxins - biosynthesis Fundamental and applied biological sciences. Psychology Gene Expression Profiling Gene Expression Regulation, Bacterial Genotype Humans Microbiology Miscellaneous Repressor Proteins - metabolism Sequence Analysis, DNA Signal Transduction Streptococcal Infections - microbiology Streptococcus pyogenes Streptococcus pyogenes - classification Streptococcus pyogenes - genetics Streptococcus pyogenes - isolation & purification Streptococcus pyogenes - physiology |
| title | emm1/Sequence Type 28 Strains of Group A Streptococci That Express covR at Early Stationary Phase Are Associated with Increased Growth and Earlier SpeB Secretion |
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