High-Throughput Method for Rapid Induction of Prophages from Lysogens and Its Application in the Study of Shiga Toxin-Encoding Escherichia coli Strains

A high-throughput 96-well plate-based method for the rapid induction of endogenous prophages from individual bacterial strains was developed. The detection of endogenous prophages was achieved by the filtration of the culture liquor following norfloxacin induction and subsequent PCRs targeting bacte...

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Veröffentlicht in:	Applied and Environmental Microbiology 2010-04, Vol.76 (7), p.2360-2365
Hauptverfasser:	McDonald, James E, Smith, Darren L, Fogg, Paul C.M, McCarthy, Alan J, Allison, Heather E
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents - pharmacology Bacteriology Biological and medical sciences Coliphages - genetics Coliphages - growth & development E coli Escherichia coli Filtration Fundamental and applied biological sciences. Psychology Gene expression Methods Microbiology Norfloxacin - pharmacology Prophages - genetics Prophages - growth & development Sensitivity and Specificity Shiga Toxin - biosynthesis Shiga Toxin - genetics Shiga-Toxigenic Escherichia coli - drug effects Shiga-Toxigenic Escherichia coli - virology Toxins Virus Activation Viruses
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