Effect of DNA Extraction Method on the Apparent Microbial Diversity of Soil

Four extraction methods, including a novel one, were compared for their efficiencies in producing DNA from three contrasting agricultural soils. Molecular analyses (PCR-denaturing gradient gel electrophoresis [DGGE] and clone libraries) focusing on different microbial groups were used as assessment...

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Veröffentlicht in:	Applied and Environmental Microbiology 2010-05, Vol.76 (10), p.3378-3382
Hauptverfasser:	İnceoglu, Özgül, Hoogwout, Eelco F, Hill, Patrick, van Elsas, Jan Dirk
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Biodiversity Biological and medical sciences Community structure Deoxyribonucleic acid DNA DNA - chemistry DNA Fingerprinting Extraction processes Fundamental and applied biological sciences. Psychology Genetic Techniques - standards Methods Microbiology Molecular Sequence Data Molecules Phylogeny Polymerase Chain Reaction Soil Microbiology Soil microorganisms Soils
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