Effect of DNA Extraction Method on the Apparent Microbial Diversity of Soil

Four extraction methods, including a novel one, were compared for their efficiencies in producing DNA from three contrasting agricultural soils. Molecular analyses (PCR-denaturing gradient gel electrophoresis [DGGE] and clone libraries) focusing on different microbial groups were used as assessment...

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Veröffentlicht in:	Applied and Environmental Microbiology 2010-05, Vol.76 (10), p.3378-3382
Hauptverfasser:	İnceoglu, Özgül, Hoogwout, Eelco F, Hill, Patrick, van Elsas, Jan Dirk
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Biodiversity Biological and medical sciences Community structure Deoxyribonucleic acid DNA DNA - chemistry DNA Fingerprinting Extraction processes Fundamental and applied biological sciences. Psychology Genetic Techniques - standards Methods Microbiology Molecular Sequence Data Molecules Phylogeny Polymerase Chain Reaction Soil Microbiology Soil microorganisms Soils
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container_title	Applied and Environmental Microbiology
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creator	İnceoglu, Özgül Hoogwout, Eelco F Hill, Patrick van Elsas, Jan Dirk
description	Four extraction methods, including a novel one, were compared for their efficiencies in producing DNA from three contrasting agricultural soils. Molecular analyses (PCR-denaturing gradient gel electrophoresis [DGGE] and clone libraries) focusing on different microbial groups were used as assessment criteria. Per soil, the DNA yields differed between extraction methods. Clear effects of method on apparent richness and community structure were found. Actinobacterial diversity based on soil DNA produced by two divergent methods revealed that a hitherto-undescribed group was obtained by the novel method.
doi_str_mv	10.1128/AEM.02715-09
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subjects	Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Biodiversity Biological and medical sciences Community structure Deoxyribonucleic acid DNA DNA - chemistry DNA Fingerprinting Extraction processes Fundamental and applied biological sciences. Psychology Genetic Techniques - standards Methods Microbiology Molecular Sequence Data Molecules Phylogeny Polymerase Chain Reaction Soil Microbiology Soil microorganisms Soils
title	Effect of DNA Extraction Method on the Apparent Microbial Diversity of Soil
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