Dehalorespiration with Polychlorinated Biphenyls by an Anaerobic Ultramicrobacterium
Anaerobic microbial dechlorination is an important step in the detoxification and elimination of polychlorinated biphenyls (PCBs), but a microorganism capable of coupling its growth to PCB dechlorination has not been isolated. Here we describe the isolation from sediment of an ultramicrobacterium, s...
Gespeichert in:
| Veröffentlicht in: | Applied and Environmental Microbiology 2008-04, Vol.74 (7), p.2089-2094 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2094 |
|---|---|
| container_issue | 7 |
| container_start_page | 2089 |
| container_title | Applied and Environmental Microbiology |
| container_volume | 74 |
| creator | May, Harold D Miller, Greg S Kjellerup, Birthe V Sowers, Kevin R |
| description | Anaerobic microbial dechlorination is an important step in the detoxification and elimination of polychlorinated biphenyls (PCBs), but a microorganism capable of coupling its growth to PCB dechlorination has not been isolated. Here we describe the isolation from sediment of an ultramicrobacterium, strain DF-1, which is capable of dechlorinating PCBs containing double-flanked chlorines added as single congeners or as Aroclor 1260 in contaminated soil. The isolate requires Desulfovibrio spp. in coculture or cell extract for growth on hydrogen and PCB in mineral medium. This is the first microorganism in pure culture demonstrated to grow by dehalorespiration with PCBs and the first isolate shown to dechlorinate weathered commercial mixtures of PCBs in historically contaminated sediments. The ability of this isolate to grow on PCBs in contaminated sediments represents a significant breakthrough for the development of in situ treatment strategies for this class of persistent organic pollutants. |
| doi_str_mv | 10.1128/AEM.01450-07 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1128_AEM_01450_07</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1454331051</sourcerecordid><originalsourceid>FETCH-LOGICAL-c520t-fd5bb17332f8116ed37229222215b7a9ebc1c49f676203b7347ec56e3841f3853</originalsourceid><addsrcrecordid>eNpd0dtvFCEUB2BiNHatvvmso4k-OfVwG-DFZK31ktRoYveZMCyzQzMDK8zY7H8v293UCy-EnC8_DhyEnmI4w5jIt8uLr2eAGYcaxD20wKBkzSlt7qMFgFI1IQxO0KOcrwGAQSMfohMsCaEY2AJdfXC9GWJyeeuTmXwM1Y2f-up7HHa2LwUfzOTW1Xu_7V3YDblqd5UJ1TIYl2LrbbUapmRGb8vJ2MklP4-P0YPODNk9Oe6naPXx4ur8c3357dOX8-VlbTmBqe7WvG2xoJR0EuPGrakgRJGyMG-FUa612DLVNaIhQFtBmXCWN45KhjsqOT1F7w6527kd3dq6UFoZ9Db50aSdjsbrfyvB93oTf-n9NQ2IEvD6GJDiz9nlSY8-WzcMJrg4Z01AgGQKCnz5H7yOcwrlccVwJSRpcEFvDqj8Rc7JdXedYND7WekyK307K317-bO_u_-Dj8Mp4NURmGzN0CUTrM93jgBhXDX7oBcH1_tNf-OT0yaP2rhRC6ZFcVIV8_xgOhO12aSSs_pBAFMAKSRWjP4Gzt2wpg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>205978261</pqid></control><display><type>article</type><title>Dehalorespiration with Polychlorinated Biphenyls by an Anaerobic Ultramicrobacterium</title><source>American Society for Microbiology</source><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>May, Harold D ; Miller, Greg S ; Kjellerup, Birthe V ; Sowers, Kevin R</creator><creatorcontrib>May, Harold D ; Miller, Greg S ; Kjellerup, Birthe V ; Sowers, Kevin R</creatorcontrib><description>Anaerobic microbial dechlorination is an important step in the detoxification and elimination of polychlorinated biphenyls (PCBs), but a microorganism capable of coupling its growth to PCB dechlorination has not been isolated. Here we describe the isolation from sediment of an ultramicrobacterium, strain DF-1, which is capable of dechlorinating PCBs containing double-flanked chlorines added as single congeners or as Aroclor 1260 in contaminated soil. The isolate requires Desulfovibrio spp. in coculture or cell extract for growth on hydrogen and PCB in mineral medium. This is the first microorganism in pure culture demonstrated to grow by dehalorespiration with PCBs and the first isolate shown to dechlorinate weathered commercial mixtures of PCBs in historically contaminated sediments. The ability of this isolate to grow on PCBs in contaminated sediments represents a significant breakthrough for the development of in situ treatment strategies for this class of persistent organic pollutants.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.01450-07</identifier><identifier>PMID: 18223104</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Anaerobiosis ; Aroclors - metabolism ; Bacteria ; Bacteria, Anaerobic - metabolism ; Biodegradation, Environmental ; Biological and medical sciences ; Cell culture ; Chlorine - metabolism ; Contaminated sediments ; Desulfitobacterium - enzymology ; Desulfitobacterium - genetics ; Desulfitobacterium - metabolism ; Desulfovibrio ; Environmental Microbiology ; Fundamental and applied biological sciences. Psychology ; Microbiology ; PCB ; Polychlorinated biphenyls ; Polychlorinated Biphenyls - metabolism ; Soil contamination ; Soil Microbiology ; Soil Pollutants - metabolism</subject><ispartof>Applied and Environmental Microbiology, 2008-04, Vol.74 (7), p.2089-2094</ispartof><rights>2008 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Apr 2008</rights><rights>Copyright © 2008, American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c520t-fd5bb17332f8116ed37229222215b7a9ebc1c49f676203b7347ec56e3841f3853</citedby><cites>FETCH-LOGICAL-c520t-fd5bb17332f8116ed37229222215b7a9ebc1c49f676203b7347ec56e3841f3853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2292607/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2292607/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20245967$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18223104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>May, Harold D</creatorcontrib><creatorcontrib>Miller, Greg S</creatorcontrib><creatorcontrib>Kjellerup, Birthe V</creatorcontrib><creatorcontrib>Sowers, Kevin R</creatorcontrib><title>Dehalorespiration with Polychlorinated Biphenyls by an Anaerobic Ultramicrobacterium</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Anaerobic microbial dechlorination is an important step in the detoxification and elimination of polychlorinated biphenyls (PCBs), but a microorganism capable of coupling its growth to PCB dechlorination has not been isolated. Here we describe the isolation from sediment of an ultramicrobacterium, strain DF-1, which is capable of dechlorinating PCBs containing double-flanked chlorines added as single congeners or as Aroclor 1260 in contaminated soil. The isolate requires Desulfovibrio spp. in coculture or cell extract for growth on hydrogen and PCB in mineral medium. This is the first microorganism in pure culture demonstrated to grow by dehalorespiration with PCBs and the first isolate shown to dechlorinate weathered commercial mixtures of PCBs in historically contaminated sediments. The ability of this isolate to grow on PCBs in contaminated sediments represents a significant breakthrough for the development of in situ treatment strategies for this class of persistent organic pollutants.</description><subject>Anaerobiosis</subject><subject>Aroclors - metabolism</subject><subject>Bacteria</subject><subject>Bacteria, Anaerobic - metabolism</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Cell culture</subject><subject>Chlorine - metabolism</subject><subject>Contaminated sediments</subject><subject>Desulfitobacterium - enzymology</subject><subject>Desulfitobacterium - genetics</subject><subject>Desulfitobacterium - metabolism</subject><subject>Desulfovibrio</subject><subject>Environmental Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbiology</subject><subject>PCB</subject><subject>Polychlorinated biphenyls</subject><subject>Polychlorinated Biphenyls - metabolism</subject><subject>Soil contamination</subject><subject>Soil Microbiology</subject><subject>Soil Pollutants - metabolism</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0dtvFCEUB2BiNHatvvmso4k-OfVwG-DFZK31ktRoYveZMCyzQzMDK8zY7H8v293UCy-EnC8_DhyEnmI4w5jIt8uLr2eAGYcaxD20wKBkzSlt7qMFgFI1IQxO0KOcrwGAQSMfohMsCaEY2AJdfXC9GWJyeeuTmXwM1Y2f-up7HHa2LwUfzOTW1Xu_7V3YDblqd5UJ1TIYl2LrbbUapmRGb8vJ2MklP4-P0YPODNk9Oe6naPXx4ur8c3357dOX8-VlbTmBqe7WvG2xoJR0EuPGrakgRJGyMG-FUa612DLVNaIhQFtBmXCWN45KhjsqOT1F7w6527kd3dq6UFoZ9Db50aSdjsbrfyvB93oTf-n9NQ2IEvD6GJDiz9nlSY8-WzcMJrg4Z01AgGQKCnz5H7yOcwrlccVwJSRpcEFvDqj8Rc7JdXedYND7WekyK307K317-bO_u_-Dj8Mp4NURmGzN0CUTrM93jgBhXDX7oBcH1_tNf-OT0yaP2rhRC6ZFcVIV8_xgOhO12aSSs_pBAFMAKSRWjP4Gzt2wpg</recordid><startdate>20080401</startdate><enddate>20080401</enddate><creator>May, Harold D</creator><creator>Miller, Greg S</creator><creator>Kjellerup, Birthe V</creator><creator>Sowers, Kevin R</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7TV</scope><scope>5PM</scope></search><sort><creationdate>20080401</creationdate><title>Dehalorespiration with Polychlorinated Biphenyls by an Anaerobic Ultramicrobacterium</title><author>May, Harold D ; Miller, Greg S ; Kjellerup, Birthe V ; Sowers, Kevin R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c520t-fd5bb17332f8116ed37229222215b7a9ebc1c49f676203b7347ec56e3841f3853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Anaerobiosis</topic><topic>Aroclors - metabolism</topic><topic>Bacteria</topic><topic>Bacteria, Anaerobic - metabolism</topic><topic>Biodegradation, Environmental</topic><topic>Biological and medical sciences</topic><topic>Cell culture</topic><topic>Chlorine - metabolism</topic><topic>Contaminated sediments</topic><topic>Desulfitobacterium - enzymology</topic><topic>Desulfitobacterium - genetics</topic><topic>Desulfitobacterium - metabolism</topic><topic>Desulfovibrio</topic><topic>Environmental Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbiology</topic><topic>PCB</topic><topic>Polychlorinated biphenyls</topic><topic>Polychlorinated Biphenyls - metabolism</topic><topic>Soil contamination</topic><topic>Soil Microbiology</topic><topic>Soil Pollutants - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>May, Harold D</creatorcontrib><creatorcontrib>Miller, Greg S</creatorcontrib><creatorcontrib>Kjellerup, Birthe V</creatorcontrib><creatorcontrib>Sowers, Kevin R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>May, Harold D</au><au>Miller, Greg S</au><au>Kjellerup, Birthe V</au><au>Sowers, Kevin R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dehalorespiration with Polychlorinated Biphenyls by an Anaerobic Ultramicrobacterium</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2008-04-01</date><risdate>2008</risdate><volume>74</volume><issue>7</issue><spage>2089</spage><epage>2094</epage><pages>2089-2094</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Anaerobic microbial dechlorination is an important step in the detoxification and elimination of polychlorinated biphenyls (PCBs), but a microorganism capable of coupling its growth to PCB dechlorination has not been isolated. Here we describe the isolation from sediment of an ultramicrobacterium, strain DF-1, which is capable of dechlorinating PCBs containing double-flanked chlorines added as single congeners or as Aroclor 1260 in contaminated soil. The isolate requires Desulfovibrio spp. in coculture or cell extract for growth on hydrogen and PCB in mineral medium. This is the first microorganism in pure culture demonstrated to grow by dehalorespiration with PCBs and the first isolate shown to dechlorinate weathered commercial mixtures of PCBs in historically contaminated sediments. The ability of this isolate to grow on PCBs in contaminated sediments represents a significant breakthrough for the development of in situ treatment strategies for this class of persistent organic pollutants.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>18223104</pmid><doi>10.1128/AEM.01450-07</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0099-2240 |
| ispartof | Applied and Environmental Microbiology, 2008-04, Vol.74 (7), p.2089-2094 |
| issn | 0099-2240 1098-5336 |
| language | eng |
| recordid | cdi_crossref_primary_10_1128_AEM_01450_07 |
| source | American Society for Microbiology; MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Anaerobiosis Aroclors - metabolism Bacteria Bacteria, Anaerobic - metabolism Biodegradation, Environmental Biological and medical sciences Cell culture Chlorine - metabolism Contaminated sediments Desulfitobacterium - enzymology Desulfitobacterium - genetics Desulfitobacterium - metabolism Desulfovibrio Environmental Microbiology Fundamental and applied biological sciences. Psychology Microbiology PCB Polychlorinated biphenyls Polychlorinated Biphenyls - metabolism Soil contamination Soil Microbiology Soil Pollutants - metabolism |
| title | Dehalorespiration with Polychlorinated Biphenyls by an Anaerobic Ultramicrobacterium |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-19T00%3A49%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dehalorespiration%20with%20Polychlorinated%20Biphenyls%20by%20an%20Anaerobic%20Ultramicrobacterium&rft.jtitle=Applied%20and%20Environmental%20Microbiology&rft.au=May,%20Harold%20D&rft.date=2008-04-01&rft.volume=74&rft.issue=7&rft.spage=2089&rft.epage=2094&rft.pages=2089-2094&rft.issn=0099-2240&rft.eissn=1098-5336&rft.coden=AEMIDF&rft_id=info:doi/10.1128/AEM.01450-07&rft_dat=%3Cproquest_cross%3E1454331051%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=205978261&rft_id=info:pmid/18223104&rfr_iscdi=true |