Chromosomally Located traHIJKCLMN Operon Encoding a Putative Type IV Secretion System Is Involved in the Virulence of Yersinia ruckeri
Nucleotide sequence analysis of the region surrounding the pIVET8 insertion site in Yersinia ruckeri 150RiviXII, previously selected by in vivo expression technology (IVET), revealed the presence of eight genes (traHIJKCLMN [hereafter referred to collectively as the tra operon or tra cluster]), whic...
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| Veröffentlicht in: | Applied and Environmental Microbiology 2009-02, Vol.75 (4), p.937-945 |
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| creator | Méndez, J Fernández, L Menéndez, A Reimundo, P Pérez-Pascual, D Navais, R Guijarro, J.A |
| description | Nucleotide sequence analysis of the region surrounding the pIVET8 insertion site in Yersinia ruckeri 150RiviXII, previously selected by in vivo expression technology (IVET), revealed the presence of eight genes (traHIJKCLMN [hereafter referred to collectively as the tra operon or tra cluster]), which are similar both in sequence and organization to the tra operon cluster found in the virulence-related plasmid pADAP from Serratia entomophila. Interestingly, the tra cluster of Y. ruckeri is chromosomally encoded, and no similar tra cluster has been identified yet in the genomic analysis of human pathogenic yersiniae. A traI insertional mutant was obtained by homologous recombination. Coinfection experiments with the mutant and the parental strain, as well as 50% lethal dose determinations, indicate that this operon is involved in the virulence of this bacterium. All of these results suggest the implication of the tra cluster in a virulence-related type IV secretion/transfer system. Reverse transcriptase PCR studies showed that this cluster is transcribed as an operon from a putative promoter located upstream of traH and that the mutation of traI had a polar effect. A traI::lacZY transcriptional fusion displayed higher expression levels at 18°C, the temperature of occurrence of the disease, and under nutrient-limiting conditions. PCR detection analysis indicated that the tra cluster is present in 15 Y. ruckeri strains from different origins and with different plasmid profiles. The results obtained in the present study support the conclusion, already suggested by different authors, that Y. ruckeri is a very homogeneous species that is quite different from the other members of the genus YERSINIA: |
| doi_str_mv | 10.1128/AEM.01377-08 |
| format | Article |
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Interestingly, the tra cluster of Y. ruckeri is chromosomally encoded, and no similar tra cluster has been identified yet in the genomic analysis of human pathogenic yersiniae. A traI insertional mutant was obtained by homologous recombination. Coinfection experiments with the mutant and the parental strain, as well as 50% lethal dose determinations, indicate that this operon is involved in the virulence of this bacterium. All of these results suggest the implication of the tra cluster in a virulence-related type IV secretion/transfer system. Reverse transcriptase PCR studies showed that this cluster is transcribed as an operon from a putative promoter located upstream of traH and that the mutation of traI had a polar effect. A traI::lacZY transcriptional fusion displayed higher expression levels at 18°C, the temperature of occurrence of the disease, and under nutrient-limiting conditions. PCR detection analysis indicated that the tra cluster is present in 15 Y. ruckeri strains from different origins and with different plasmid profiles. The results obtained in the present study support the conclusion, already suggested by different authors, that Y. ruckeri is a very homogeneous species that is quite different from the other members of the genus YERSINIA:</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/AEM.01377-08</identifier><identifier>PMID: 19088314</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biological and medical sciences ; Chromosomes ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; Fish Diseases - microbiology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Profiling ; Gene Order ; Genes ; Genetic research ; Genetics and Molecular Biology ; Genomic analysis ; Genomics ; Lethal Dose 50 ; Membrane Transport Proteins - genetics ; Membrane Transport Proteins - metabolism ; Microbiology ; Molecular Sequence Data ; Mutagenesis, Insertional ; Mutation ; Oncorhynchus mykiss - microbiology ; Operon ; Pathogens ; Protein Transport ; Sequence Analysis, DNA ; Sequence Homology ; Serratia - genetics ; Serratia entomophila ; Temperature ; Virulence ; Virulence Factors - genetics ; Virulence Factors - metabolism ; Yersinia - genetics ; Yersinia - pathogenicity</subject><ispartof>Applied and Environmental Microbiology, 2009-02, Vol.75 (4), p.937-945</ispartof><rights>2009 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Feb 2009</rights><rights>Copyright © 2009, American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c524t-f11dab380e905138d49d2d50626c7abfd2f1df7d387d360334b3fdc983f95a813</citedby><cites>FETCH-LOGICAL-c524t-f11dab380e905138d49d2d50626c7abfd2f1df7d387d360334b3fdc983f95a813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2643577/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2643577/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21139836$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19088314$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Méndez, J</creatorcontrib><creatorcontrib>Fernández, L</creatorcontrib><creatorcontrib>Menéndez, A</creatorcontrib><creatorcontrib>Reimundo, P</creatorcontrib><creatorcontrib>Pérez-Pascual, D</creatorcontrib><creatorcontrib>Navais, R</creatorcontrib><creatorcontrib>Guijarro, J.A</creatorcontrib><title>Chromosomally Located traHIJKCLMN Operon Encoding a Putative Type IV Secretion System Is Involved in the Virulence of Yersinia ruckeri</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Nucleotide sequence analysis of the region surrounding the pIVET8 insertion site in Yersinia ruckeri 150RiviXII, previously selected by in vivo expression technology (IVET), revealed the presence of eight genes (traHIJKCLMN [hereafter referred to collectively as the tra operon or tra cluster]), which are similar both in sequence and organization to the tra operon cluster found in the virulence-related plasmid pADAP from Serratia entomophila. Interestingly, the tra cluster of Y. ruckeri is chromosomally encoded, and no similar tra cluster has been identified yet in the genomic analysis of human pathogenic yersiniae. A traI insertional mutant was obtained by homologous recombination. Coinfection experiments with the mutant and the parental strain, as well as 50% lethal dose determinations, indicate that this operon is involved in the virulence of this bacterium. All of these results suggest the implication of the tra cluster in a virulence-related type IV secretion/transfer system. Reverse transcriptase PCR studies showed that this cluster is transcribed as an operon from a putative promoter located upstream of traH and that the mutation of traI had a polar effect. A traI::lacZY transcriptional fusion displayed higher expression levels at 18°C, the temperature of occurrence of the disease, and under nutrient-limiting conditions. PCR detection analysis indicated that the tra cluster is present in 15 Y. ruckeri strains from different origins and with different plasmid profiles. The results obtained in the present study support the conclusion, already suggested by different authors, that Y. ruckeri is a very homogeneous species that is quite different from the other members of the genus YERSINIA:</description><subject>Animals</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biological and medical sciences</subject><subject>Chromosomes</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Fish Diseases - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Profiling</subject><subject>Gene Order</subject><subject>Genes</subject><subject>Genetic research</subject><subject>Genetics and Molecular Biology</subject><subject>Genomic analysis</subject><subject>Genomics</subject><subject>Lethal Dose 50</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Insertional</subject><subject>Mutation</subject><subject>Oncorhynchus mykiss - microbiology</subject><subject>Operon</subject><subject>Pathogens</subject><subject>Protein Transport</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology</subject><subject>Serratia - genetics</subject><subject>Serratia entomophila</subject><subject>Temperature</subject><subject>Virulence</subject><subject>Virulence Factors - genetics</subject><subject>Virulence Factors - metabolism</subject><subject>Yersinia - genetics</subject><subject>Yersinia - pathogenicity</subject><issn>0099-2240</issn><issn>1098-5336</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0ctuEzEUBuARAtG0sGMNFhKsmOLLXOwNUhWlNJBSpLSVWFmO5zhxO2MHeyYoL8Bz45CoXBaWLfnT73N8suwFwaeEUP7-bHJ5igmr6xzzR9mIYMHzkrHqcTbCWIic0gIfZccx3mGMC1zxp9kREZhzRopR9nO8Cr7z0Xeqbbdo5rXqoUF9UBfTT5_Hs8sv6GoNwTs0cdo31i2RQl-HXvV2A-h6uwY0vUVz0AF6m9R8G3vo0DSiqdv4dpOyrEP9CtCtDUMLTgPyBn2DEK2zCoVB30Owz7InRrURnh_2k-zmfHI9vshnVx-n47NZrkta9LkhpFELxjEIXBLGm0I0tClxRStdq4VpqCGNqRvG06owY8WCmUYLzowoFSfsJPuwz10Piw4aDS512sp1sJ0KW-mVlf_eOLuSS7-RtCpYWdcp4O0hIPjvA8RedjZqaFvlwA9RUlIIUbEiwdf_wTs_BJeakxSXiZR1ldC7PdLBxxjAPFRCsNxNV6bpyt_TlZgn_vLv6v_gwzgTeHMAKmrVmqCctvHBUUJY-ovdu2jvVna5-mEDSBU7qaCTdSkLKdiu0Vd7YpSXahlSzM2cplIwKQUm6fALWwbBzg</recordid><startdate>200902</startdate><enddate>200902</enddate><creator>Méndez, J</creator><creator>Fernández, L</creator><creator>Menéndez, A</creator><creator>Reimundo, P</creator><creator>Pérez-Pascual, D</creator><creator>Navais, R</creator><creator>Guijarro, J.A</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>5PM</scope></search><sort><creationdate>200902</creationdate><title>Chromosomally Located traHIJKCLMN Operon Encoding a Putative Type IV Secretion System Is Involved in the Virulence of Yersinia ruckeri</title><author>Méndez, J ; Fernández, L ; Menéndez, A ; Reimundo, P ; Pérez-Pascual, D ; Navais, R ; Guijarro, J.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c524t-f11dab380e905138d49d2d50626c7abfd2f1df7d387d360334b3fdc983f95a813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biological and medical sciences</topic><topic>Chromosomes</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>Fish Diseases - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Profiling</topic><topic>Gene Order</topic><topic>Genes</topic><topic>Genetic research</topic><topic>Genetics and Molecular Biology</topic><topic>Genomic analysis</topic><topic>Genomics</topic><topic>Lethal Dose 50</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Insertional</topic><topic>Mutation</topic><topic>Oncorhynchus mykiss - microbiology</topic><topic>Operon</topic><topic>Pathogens</topic><topic>Protein Transport</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology</topic><topic>Serratia - genetics</topic><topic>Serratia entomophila</topic><topic>Temperature</topic><topic>Virulence</topic><topic>Virulence Factors - genetics</topic><topic>Virulence Factors - metabolism</topic><topic>Yersinia - genetics</topic><topic>Yersinia - pathogenicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Méndez, J</creatorcontrib><creatorcontrib>Fernández, L</creatorcontrib><creatorcontrib>Menéndez, A</creatorcontrib><creatorcontrib>Reimundo, P</creatorcontrib><creatorcontrib>Pérez-Pascual, D</creatorcontrib><creatorcontrib>Navais, R</creatorcontrib><creatorcontrib>Guijarro, J.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Méndez, J</au><au>Fernández, L</au><au>Menéndez, A</au><au>Reimundo, P</au><au>Pérez-Pascual, D</au><au>Navais, R</au><au>Guijarro, J.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chromosomally Located traHIJKCLMN Operon Encoding a Putative Type IV Secretion System Is Involved in the Virulence of Yersinia ruckeri</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2009-02</date><risdate>2009</risdate><volume>75</volume><issue>4</issue><spage>937</spage><epage>945</epage><pages>937-945</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><eissn>1098-6596</eissn><coden>AEMIDF</coden><abstract>Nucleotide sequence analysis of the region surrounding the pIVET8 insertion site in Yersinia ruckeri 150RiviXII, previously selected by in vivo expression technology (IVET), revealed the presence of eight genes (traHIJKCLMN [hereafter referred to collectively as the tra operon or tra cluster]), which are similar both in sequence and organization to the tra operon cluster found in the virulence-related plasmid pADAP from Serratia entomophila. Interestingly, the tra cluster of Y. ruckeri is chromosomally encoded, and no similar tra cluster has been identified yet in the genomic analysis of human pathogenic yersiniae. A traI insertional mutant was obtained by homologous recombination. Coinfection experiments with the mutant and the parental strain, as well as 50% lethal dose determinations, indicate that this operon is involved in the virulence of this bacterium. All of these results suggest the implication of the tra cluster in a virulence-related type IV secretion/transfer system. Reverse transcriptase PCR studies showed that this cluster is transcribed as an operon from a putative promoter located upstream of traH and that the mutation of traI had a polar effect. A traI::lacZY transcriptional fusion displayed higher expression levels at 18°C, the temperature of occurrence of the disease, and under nutrient-limiting conditions. PCR detection analysis indicated that the tra cluster is present in 15 Y. ruckeri strains from different origins and with different plasmid profiles. The results obtained in the present study support the conclusion, already suggested by different authors, that Y. ruckeri is a very homogeneous species that is quite different from the other members of the genus YERSINIA:</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>19088314</pmid><doi>10.1128/AEM.01377-08</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Bacterial Proteins - genetics Bacterial Proteins - metabolism Biological and medical sciences Chromosomes DNA, Bacterial - chemistry DNA, Bacterial - genetics Fish Diseases - microbiology Fundamental and applied biological sciences. Psychology Gene Expression Profiling Gene Order Genes Genetic research Genetics and Molecular Biology Genomic analysis Genomics Lethal Dose 50 Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Microbiology Molecular Sequence Data Mutagenesis, Insertional Mutation Oncorhynchus mykiss - microbiology Operon Pathogens Protein Transport Sequence Analysis, DNA Sequence Homology Serratia - genetics Serratia entomophila Temperature Virulence Virulence Factors - genetics Virulence Factors - metabolism Yersinia - genetics Yersinia - pathogenicity |
| title | Chromosomally Located traHIJKCLMN Operon Encoding a Putative Type IV Secretion System Is Involved in the Virulence of Yersinia ruckeri |
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