Conversion Shift of D-Fructose to D-Psicose for Enzyme-Catalyzed Epimerization by Addition of Borate

The conversion yield of D-psicose from D-fructose by a D-psicose 3-epimerase from Agrobacterium tumefaciens increased with increasing molar ratios of borate to fructose, up to a ratio of 0.6. The formation of the psicose-borate complex was the result of the higher binding affinity of borate for psic...

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Veröffentlicht in:	Applied and Environmental Microbiology 2008-05, Vol.74 (10), p.3008-3013
Hauptverfasser:	Kim, Nam-Hee, Kim, Hye-Jung, Kang, Dong-Il, Jeong, Ki-Woong, Lee, Jung-Kul, Kim, Yangmee, Oh, Deok-Kun
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium tumefaciens Agrobacterium tumefaciens - enzymology Bacteria Bacteriology Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Borates - chemistry Borates - metabolism Carbohydrate Epimerases - drug effects Carbohydrate Epimerases - isolation & purification Catalysis Enzymes Enzymology and Protein Engineering Fructose - metabolism Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Kinetics Magnetic Resonance Spectroscopy Methods. Procedures. Technologies Microbiology Molecular Structure Salt Sugar Temperature
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