The Structure of Importin-ß Bound to SREBP-2: Nuclear Import of a Transcription Factor
The sterol regulatory element–binding protein 2 (SREBP-2), a nuclear transcription factor that is essential for cholesterol metabolism, enters the nucleus through a direct interaction of its helix-loop-helix leucine zipper domain with importin-β. We show the crystal structure of importin-β complexed...
Gespeichert in:
| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2003-11, Vol.302 (5650), p.1571-1575 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng ; jpn |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1575 |
|---|---|
| container_issue | 5650 |
| container_start_page | 1571 |
| container_title | Science (American Association for the Advancement of Science) |
| container_volume | 302 |
| creator | Lee, Soo Jae Sekimoto, Toshihiro Yamashita, Eiki Nagoshi, Emi Nakagawa, Atsushi Imamoto, Naoko Yoshimura, Masato Sakai, Hiroaki Chong, Khoon Tee Tsukihara, Tomitake Yoneda, Yoshihiro |
| description | The sterol regulatory element–binding protein 2 (SREBP-2), a nuclear transcription factor that is essential for cholesterol metabolism, enters the nucleus through a direct interaction of its helix-loop-helix leucine zipper domain with importin-β. We show the crystal structure of importin-β complexed with the active form of SREBP-2. Importin-β uses characteristic long helices like a pair of chopsticks to interact with an SREBP-2 dimer. Importin-β changes its conformation to reveal a pseudo-twofold symmetry on its surface structure so that it can accommodate a symmetric dimer molecule. Importin-β may use a similar strategy to recognize other dimeric cargoes. |
| doi_str_mv | 10.1126/science.1088372 |
| format | Article |
| fullrecord | <record><control><sourceid>crossref</sourceid><recordid>TN_cdi_crossref_primary_10_1126_science_1088372</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_1126_science_1088372</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1524-81022d141b95380d61bbfa7e6a30a9e3934c29586c8dcdd6b444fe4e5a48d653</originalsourceid><addsrcrecordid>eNotkL1OwzAYRS0EEqUws_oF3H7-jc1Gq7ZUqgDRSIyRYzsiqI0rOxl4Gh6GF4OKTHe4R2c4CN1TmFHK1Dy7NnQuzChozQt2gSYUjCSGAb9EEwCuiIZCXqObnD8B_j7DJ-i9_Ah436fB9UMKODZ4ezzF1Lcd-fnGizh0HvcR799Wi1fCHvDz4A7BppE68xaXyXbZpfbUt7HDa-v6mG7RVWMPOdyNO0XlelUun8juZbNdPu6Io5IJoikw5qmgtZFcg1e0rhtbBGU5WBO44cIxI7Vy2jvvVS2EaIII0grtleRTNP_XuhRzTqGpTqk92vRVUajOWaoxSzVm4b8vuVcV</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>The Structure of Importin-ß Bound to SREBP-2: Nuclear Import of a Transcription Factor</title><source>American Association for the Advancement of Science</source><source>Jstor Complete Legacy</source><creator>Lee, Soo Jae ; Sekimoto, Toshihiro ; Yamashita, Eiki ; Nagoshi, Emi ; Nakagawa, Atsushi ; Imamoto, Naoko ; Yoshimura, Masato ; Sakai, Hiroaki ; Chong, Khoon Tee ; Tsukihara, Tomitake ; Yoneda, Yoshihiro</creator><creatorcontrib>Lee, Soo Jae ; Sekimoto, Toshihiro ; Yamashita, Eiki ; Nagoshi, Emi ; Nakagawa, Atsushi ; Imamoto, Naoko ; Yoshimura, Masato ; Sakai, Hiroaki ; Chong, Khoon Tee ; Tsukihara, Tomitake ; Yoneda, Yoshihiro</creatorcontrib><description>The sterol regulatory element–binding protein 2 (SREBP-2), a nuclear transcription factor that is essential for cholesterol metabolism, enters the nucleus through a direct interaction of its helix-loop-helix leucine zipper domain with importin-β. We show the crystal structure of importin-β complexed with the active form of SREBP-2. Importin-β uses characteristic long helices like a pair of chopsticks to interact with an SREBP-2 dimer. Importin-β changes its conformation to reveal a pseudo-twofold symmetry on its surface structure so that it can accommodate a symmetric dimer molecule. Importin-β may use a similar strategy to recognize other dimeric cargoes.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1088372</identifier><language>eng ; jpn</language><ispartof>Science (American Association for the Advancement of Science), 2003-11, Vol.302 (5650), p.1571-1575</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1524-81022d141b95380d61bbfa7e6a30a9e3934c29586c8dcdd6b444fe4e5a48d653</citedby><cites>FETCH-LOGICAL-c1524-81022d141b95380d61bbfa7e6a30a9e3934c29586c8dcdd6b444fe4e5a48d653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2871,2872,27901,27902</link.rule.ids></links><search><creatorcontrib>Lee, Soo Jae</creatorcontrib><creatorcontrib>Sekimoto, Toshihiro</creatorcontrib><creatorcontrib>Yamashita, Eiki</creatorcontrib><creatorcontrib>Nagoshi, Emi</creatorcontrib><creatorcontrib>Nakagawa, Atsushi</creatorcontrib><creatorcontrib>Imamoto, Naoko</creatorcontrib><creatorcontrib>Yoshimura, Masato</creatorcontrib><creatorcontrib>Sakai, Hiroaki</creatorcontrib><creatorcontrib>Chong, Khoon Tee</creatorcontrib><creatorcontrib>Tsukihara, Tomitake</creatorcontrib><creatorcontrib>Yoneda, Yoshihiro</creatorcontrib><title>The Structure of Importin-ß Bound to SREBP-2: Nuclear Import of a Transcription Factor</title><title>Science (American Association for the Advancement of Science)</title><description>The sterol regulatory element–binding protein 2 (SREBP-2), a nuclear transcription factor that is essential for cholesterol metabolism, enters the nucleus through a direct interaction of its helix-loop-helix leucine zipper domain with importin-β. We show the crystal structure of importin-β complexed with the active form of SREBP-2. Importin-β uses characteristic long helices like a pair of chopsticks to interact with an SREBP-2 dimer. Importin-β changes its conformation to reveal a pseudo-twofold symmetry on its surface structure so that it can accommodate a symmetric dimer molecule. Importin-β may use a similar strategy to recognize other dimeric cargoes.</description><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNotkL1OwzAYRS0EEqUws_oF3H7-jc1Gq7ZUqgDRSIyRYzsiqI0rOxl4Gh6GF4OKTHe4R2c4CN1TmFHK1Dy7NnQuzChozQt2gSYUjCSGAb9EEwCuiIZCXqObnD8B_j7DJ-i9_Ah436fB9UMKODZ4ezzF1Lcd-fnGizh0HvcR799Wi1fCHvDz4A7BppE68xaXyXbZpfbUt7HDa-v6mG7RVWMPOdyNO0XlelUun8juZbNdPu6Io5IJoikw5qmgtZFcg1e0rhtbBGU5WBO44cIxI7Vy2jvvVS2EaIII0grtleRTNP_XuhRzTqGpTqk92vRVUajOWaoxSzVm4b8vuVcV</recordid><startdate>20031128</startdate><enddate>20031128</enddate><creator>Lee, Soo Jae</creator><creator>Sekimoto, Toshihiro</creator><creator>Yamashita, Eiki</creator><creator>Nagoshi, Emi</creator><creator>Nakagawa, Atsushi</creator><creator>Imamoto, Naoko</creator><creator>Yoshimura, Masato</creator><creator>Sakai, Hiroaki</creator><creator>Chong, Khoon Tee</creator><creator>Tsukihara, Tomitake</creator><creator>Yoneda, Yoshihiro</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20031128</creationdate><title>The Structure of Importin-ß Bound to SREBP-2: Nuclear Import of a Transcription Factor</title><author>Lee, Soo Jae ; Sekimoto, Toshihiro ; Yamashita, Eiki ; Nagoshi, Emi ; Nakagawa, Atsushi ; Imamoto, Naoko ; Yoshimura, Masato ; Sakai, Hiroaki ; Chong, Khoon Tee ; Tsukihara, Tomitake ; Yoneda, Yoshihiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1524-81022d141b95380d61bbfa7e6a30a9e3934c29586c8dcdd6b444fe4e5a48d653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng ; jpn</language><creationdate>2003</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Soo Jae</creatorcontrib><creatorcontrib>Sekimoto, Toshihiro</creatorcontrib><creatorcontrib>Yamashita, Eiki</creatorcontrib><creatorcontrib>Nagoshi, Emi</creatorcontrib><creatorcontrib>Nakagawa, Atsushi</creatorcontrib><creatorcontrib>Imamoto, Naoko</creatorcontrib><creatorcontrib>Yoshimura, Masato</creatorcontrib><creatorcontrib>Sakai, Hiroaki</creatorcontrib><creatorcontrib>Chong, Khoon Tee</creatorcontrib><creatorcontrib>Tsukihara, Tomitake</creatorcontrib><creatorcontrib>Yoneda, Yoshihiro</creatorcontrib><collection>CrossRef</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Soo Jae</au><au>Sekimoto, Toshihiro</au><au>Yamashita, Eiki</au><au>Nagoshi, Emi</au><au>Nakagawa, Atsushi</au><au>Imamoto, Naoko</au><au>Yoshimura, Masato</au><au>Sakai, Hiroaki</au><au>Chong, Khoon Tee</au><au>Tsukihara, Tomitake</au><au>Yoneda, Yoshihiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Structure of Importin-ß Bound to SREBP-2: Nuclear Import of a Transcription Factor</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><date>2003-11-28</date><risdate>2003</risdate><volume>302</volume><issue>5650</issue><spage>1571</spage><epage>1575</epage><pages>1571-1575</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><abstract>The sterol regulatory element–binding protein 2 (SREBP-2), a nuclear transcription factor that is essential for cholesterol metabolism, enters the nucleus through a direct interaction of its helix-loop-helix leucine zipper domain with importin-β. We show the crystal structure of importin-β complexed with the active form of SREBP-2. Importin-β uses characteristic long helices like a pair of chopsticks to interact with an SREBP-2 dimer. Importin-β changes its conformation to reveal a pseudo-twofold symmetry on its surface structure so that it can accommodate a symmetric dimer molecule. Importin-β may use a similar strategy to recognize other dimeric cargoes.</abstract><doi>10.1126/science.1088372</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 2003-11, Vol.302 (5650), p.1571-1575 |
| issn | 0036-8075 1095-9203 |
| language | eng ; jpn |
| recordid | cdi_crossref_primary_10_1126_science_1088372 |
| source | American Association for the Advancement of Science; Jstor Complete Legacy |
| title | The Structure of Importin-ß Bound to SREBP-2: Nuclear Import of a Transcription Factor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T09%3A56%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-crossref&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Structure%20of%20Importin-%C3%9F%20Bound%20to%20SREBP-2:%20Nuclear%20Import%20of%20a%20Transcription%20Factor&rft.jtitle=Science%20(American%20Association%20for%20the%20Advancement%20of%20Science)&rft.au=Lee,%20Soo%20Jae&rft.date=2003-11-28&rft.volume=302&rft.issue=5650&rft.spage=1571&rft.epage=1575&rft.pages=1571-1575&rft.issn=0036-8075&rft.eissn=1095-9203&rft_id=info:doi/10.1126/science.1088372&rft_dat=%3Ccrossref%3E10_1126_science_1088372%3C/crossref%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |