Unanimous Model for Describing the Fast Bioluminescence Kinetics of Ca 2+ -regulated Photoproteins of Different Organisms

Upon binding their metal ion cofactors, Ca -regulated photoproteins display a rapid increase of light signal, which reaches its peak within milliseconds. In the present study, we investigate bioluminescence kinetics of the entire photoprotein family. All five recombinant hydromedusan Ca -regulated p...

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Veröffentlicht in:Photochemistry and photobiology 2017-03, Vol.93 (2), p.495-502
Hauptverfasser: Eremeeva, Elena V, Bartsev, Sergey I, van Berkel, Willem J H, Vysotski, Eugene S
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creator Eremeeva, Elena V
Bartsev, Sergey I
van Berkel, Willem J H
Vysotski, Eugene S
description Upon binding their metal ion cofactors, Ca -regulated photoproteins display a rapid increase of light signal, which reaches its peak within milliseconds. In the present study, we investigate bioluminescence kinetics of the entire photoprotein family. All five recombinant hydromedusan Ca -regulated photoproteins-aequorin from Aequorea victoria, clytin from Clytia gregaria, mitrocomin from Mitrocoma cellularia and obelins from Obelia longissima and Obelia geniculata-demonstrate the same bioluminescent kinetics pattern. Based on these findings, for the first time we propose a unanimous kinetic model describing the bioluminescence mechanism of Ca -regulated photoproteins.
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title Unanimous Model for Describing the Fast Bioluminescence Kinetics of Ca 2+ -regulated Photoproteins of Different Organisms
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