Expressions and inhibitory functions of CD 300a receptors on purified human basophils
The inhibitory myeloid immunoglobulin receptor CD 300a ( IR p60) has been shown to downregulate mast cell and eosinophil activities, thereby serving as a potential target for inhibiting allergic effector cell input in allergy. Our aims were to study the expression and functional properties of this r...
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| Veröffentlicht in: | Experimental dermatology 2012-11, Vol.21 (11), p.884-885 |
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| container_title | Experimental dermatology |
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| creator | Gibbs, Bernhard F. Sabato, Vito Bridts, Chris H. Ebo, Didier G. Ben‐Zimra, Micha Levi‐Schaffer, Francesca |
| description | The inhibitory myeloid immunoglobulin receptor
CD
300a (
IR
p60) has been shown to downregulate mast cell and eosinophil activities, thereby serving as a potential target for inhibiting allergic effector cell input in allergy. Our aims were to study the expression and functional properties of this receptor in purified human basophils, cells that crucially contribute to
T
h2‐type immunity and allergy. Basophils homogeneously expressed
CD
300a as well as the inhibitory receptor
CD
200
R
on their cell surface, and these expressions increased after anti‐
I
g
E
stimulation. Ig
E
‐mediated basophil degranulation was also significantly inhibited by crosslinking of either
CD
200R or
CD
300a (by 90% and 50%, respectively). Inhibitory
SHIP
‐1 phosphorylations were also induced by
CD
200
R
and
CD
300a, although they were not noticeably increased by
I
g
E
‐dependent activation. We conclude that both
CD
200
R
and
CD
300a play a role in reducing
I
g
E
‐mediated basophil function and may crucially govern the known differential activities of these cells
in vivo
. |
| doi_str_mv | 10.1111/exd.12018 |
| format | Article |
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CD
300a (
IR
p60) has been shown to downregulate mast cell and eosinophil activities, thereby serving as a potential target for inhibiting allergic effector cell input in allergy. Our aims were to study the expression and functional properties of this receptor in purified human basophils, cells that crucially contribute to
T
h2‐type immunity and allergy. Basophils homogeneously expressed
CD
300a as well as the inhibitory receptor
CD
200
R
on their cell surface, and these expressions increased after anti‐
I
g
E
stimulation. Ig
E
‐mediated basophil degranulation was also significantly inhibited by crosslinking of either
CD
200R or
CD
300a (by 90% and 50%, respectively). Inhibitory
SHIP
‐1 phosphorylations were also induced by
CD
200
R
and
CD
300a, although they were not noticeably increased by
I
g
E
‐dependent activation. We conclude that both
CD
200
R
and
CD
300a play a role in reducing
I
g
E
‐mediated basophil function and may crucially govern the known differential activities of these cells
in vivo
.</description><identifier>ISSN: 0906-6705</identifier><identifier>EISSN: 1600-0625</identifier><identifier>DOI: 10.1111/exd.12018</identifier><language>eng</language><ispartof>Experimental dermatology, 2012-11, Vol.21 (11), p.884-885</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c748-dfce3789f01dd81a72e38a2470f9239119fbb7a2fd976502316021683f89c85b3</citedby><cites>FETCH-LOGICAL-c748-dfce3789f01dd81a72e38a2470f9239119fbb7a2fd976502316021683f89c85b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Gibbs, Bernhard F.</creatorcontrib><creatorcontrib>Sabato, Vito</creatorcontrib><creatorcontrib>Bridts, Chris H.</creatorcontrib><creatorcontrib>Ebo, Didier G.</creatorcontrib><creatorcontrib>Ben‐Zimra, Micha</creatorcontrib><creatorcontrib>Levi‐Schaffer, Francesca</creatorcontrib><title>Expressions and inhibitory functions of CD 300a receptors on purified human basophils</title><title>Experimental dermatology</title><description>The inhibitory myeloid immunoglobulin receptor
CD
300a (
IR
p60) has been shown to downregulate mast cell and eosinophil activities, thereby serving as a potential target for inhibiting allergic effector cell input in allergy. Our aims were to study the expression and functional properties of this receptor in purified human basophils, cells that crucially contribute to
T
h2‐type immunity and allergy. Basophils homogeneously expressed
CD
300a as well as the inhibitory receptor
CD
200
R
on their cell surface, and these expressions increased after anti‐
I
g
E
stimulation. Ig
E
‐mediated basophil degranulation was also significantly inhibited by crosslinking of either
CD
200R or
CD
300a (by 90% and 50%, respectively). Inhibitory
SHIP
‐1 phosphorylations were also induced by
CD
200
R
and
CD
300a, although they were not noticeably increased by
I
g
E
‐dependent activation. We conclude that both
CD
200
R
and
CD
300a play a role in reducing
I
g
E
‐mediated basophil function and may crucially govern the known differential activities of these cells
in vivo
.</description><issn>0906-6705</issn><issn>1600-0625</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNotkE1Lw0AYhBdRMFYP_oO9ekh9391mP44SaxUKXuo5bPaDrLRJ2G2g_ffG6lwGZmBgHkIeEZY469mf3BIZoLoiBQqAEgSrrkkBGkQpJFS35C7nbwCUXFYF-VqfxuRzjkOfqekdjX0X23gc0pmGqbfHSzEEWr9SDmBo8taPcz2HPR2nFEP0jnbTwfS0NXkYu7jP9-QmmH32D_--ILu39a5-L7efm4_6ZVtauVKlC9ZzqXQAdE6hkcxzZdhKQtCMa0Qd2lYaFpyWogLG50MMheJBaauqli_I09-sTUPOyYdmTPFg0rlBaH5xNDOO5oKD_wCrLVKR</recordid><startdate>201211</startdate><enddate>201211</enddate><creator>Gibbs, Bernhard F.</creator><creator>Sabato, Vito</creator><creator>Bridts, Chris H.</creator><creator>Ebo, Didier G.</creator><creator>Ben‐Zimra, Micha</creator><creator>Levi‐Schaffer, Francesca</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>201211</creationdate><title>Expressions and inhibitory functions of CD 300a receptors on purified human basophils</title><author>Gibbs, Bernhard F. ; Sabato, Vito ; Bridts, Chris H. ; Ebo, Didier G. ; Ben‐Zimra, Micha ; Levi‐Schaffer, Francesca</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c748-dfce3789f01dd81a72e38a2470f9239119fbb7a2fd976502316021683f89c85b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gibbs, Bernhard F.</creatorcontrib><creatorcontrib>Sabato, Vito</creatorcontrib><creatorcontrib>Bridts, Chris H.</creatorcontrib><creatorcontrib>Ebo, Didier G.</creatorcontrib><creatorcontrib>Ben‐Zimra, Micha</creatorcontrib><creatorcontrib>Levi‐Schaffer, Francesca</creatorcontrib><collection>CrossRef</collection><jtitle>Experimental dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gibbs, Bernhard F.</au><au>Sabato, Vito</au><au>Bridts, Chris H.</au><au>Ebo, Didier G.</au><au>Ben‐Zimra, Micha</au><au>Levi‐Schaffer, Francesca</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expressions and inhibitory functions of CD 300a receptors on purified human basophils</atitle><jtitle>Experimental dermatology</jtitle><date>2012-11</date><risdate>2012</risdate><volume>21</volume><issue>11</issue><spage>884</spage><epage>885</epage><pages>884-885</pages><issn>0906-6705</issn><eissn>1600-0625</eissn><abstract>The inhibitory myeloid immunoglobulin receptor
CD
300a (
IR
p60) has been shown to downregulate mast cell and eosinophil activities, thereby serving as a potential target for inhibiting allergic effector cell input in allergy. Our aims were to study the expression and functional properties of this receptor in purified human basophils, cells that crucially contribute to
T
h2‐type immunity and allergy. Basophils homogeneously expressed
CD
300a as well as the inhibitory receptor
CD
200
R
on their cell surface, and these expressions increased after anti‐
I
g
E
stimulation. Ig
E
‐mediated basophil degranulation was also significantly inhibited by crosslinking of either
CD
200R or
CD
300a (by 90% and 50%, respectively). Inhibitory
SHIP
‐1 phosphorylations were also induced by
CD
200
R
and
CD
300a, although they were not noticeably increased by
I
g
E
‐dependent activation. We conclude that both
CD
200
R
and
CD
300a play a role in reducing
I
g
E
‐mediated basophil function and may crucially govern the known differential activities of these cells
in vivo
.</abstract><doi>10.1111/exd.12018</doi><tpages>2</tpages></addata></record> |
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| identifier | ISSN: 0906-6705 |
| ispartof | Experimental dermatology, 2012-11, Vol.21 (11), p.884-885 |
| issn | 0906-6705 1600-0625 |
| language | eng |
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| source | Access via Wiley Online Library |
| title | Expressions and inhibitory functions of CD 300a receptors on purified human basophils |
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