Comparative analysis between RQ ‐ PCR and digital droplet PCR of BCL 2/ IGH gene rearrangement in the peripheral blood and bone marrow of early stage follicular lymphoma
BCL 2/ IGH rearrangements were analysed by polymerase chain reaction ( PCR ) at diagnosis in paired peripheral blood ( PB ) and bone marrow ( BM ) samples from 67 patients with stage I/ II follicular lymphoma ( FL ). Real time quantitative PCR ( RQ ‐ PCR ) and digital droplet PCR (dd PCR ) were perf...
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Veröffentlicht in: | British journal of haematology 2017-05, Vol.177 (4), p.588-596 |
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creator | Cavalli, Marzia De Novi, Lucia Anna Della Starza, Irene Cappelli, Luca Vincenzo Nunes, Vittorio Pulsoni, Alessandro Del Giudice, Ilaria Guarini, Anna Foà, Robin |
description | BCL
2/
IGH
rearrangements were analysed by polymerase chain reaction (
PCR
) at diagnosis in paired peripheral blood (
PB
) and bone marrow (
BM
) samples from 67 patients with stage I/
II
follicular lymphoma (
FL
). Real time quantitative
PCR
(
RQ
‐
PCR
) and digital droplet
PCR
(dd
PCR
) were performed in cases with a major breakpoint region (MBR+) at diagnosis and after localized radiotherapy and rituximab administration in order to investigate the applicability of dd
PCR
. The overall dd
PCR
/
RQ
‐
PCR
concordance was 81·9% (113/138 samples) and 97·5% in the 40/138 with quantifiable disease (
RQ
‐
PCR
≥10
−5
). At baseline, dd
PCR
allowed the recovery of a MBR+ marker in 8/18 (44·4%) samples that resulted MBR‐negative/minor cluster region‐negative/minor
BCL2
‐negative by qualitative
PCR
. Moreover, the tumour burden at diagnosis significantly predicted progression‐free survival (
PSF
) only when quantified by dd
PCR
. Paired
PB
and
BM
samples analysis demonstrated a high concordance in the detection of
BCL
2/IGH+
cells by qualitative and quantitative methods; in particular, 40/62 samples were positive by dd
PCR
(25 PB+/BM+; 9 PB+/BM−; 6 PB−/BM+), with 34/40 (85%) identified by the study of
PB
only. In conclusion, in localized
FL
, dd
PCR
is a promising tool for monitoring minimal residual disease (MRD) that is at least comparable to
RQ
‐
PCR
and potentially more accurate.
PB
is a suitable source for serial
BCL
2/
IGH
MRD
assessments, regardless of the methodology utilized. |
doi_str_mv | 10.1111/bjh.14616 |
format | Article |
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2/
IGH
rearrangements were analysed by polymerase chain reaction (
PCR
) at diagnosis in paired peripheral blood (
PB
) and bone marrow (
BM
) samples from 67 patients with stage I/
II
follicular lymphoma (
FL
). Real time quantitative
PCR
(
RQ
‐
PCR
) and digital droplet
PCR
(dd
PCR
) were performed in cases with a major breakpoint region (MBR+) at diagnosis and after localized radiotherapy and rituximab administration in order to investigate the applicability of dd
PCR
. The overall dd
PCR
/
RQ
‐
PCR
concordance was 81·9% (113/138 samples) and 97·5% in the 40/138 with quantifiable disease (
RQ
‐
PCR
≥10
−5
). At baseline, dd
PCR
allowed the recovery of a MBR+ marker in 8/18 (44·4%) samples that resulted MBR‐negative/minor cluster region‐negative/minor
BCL2
‐negative by qualitative
PCR
. Moreover, the tumour burden at diagnosis significantly predicted progression‐free survival (
PSF
) only when quantified by dd
PCR
. Paired
PB
and
BM
samples analysis demonstrated a high concordance in the detection of
BCL
2/IGH+
cells by qualitative and quantitative methods; in particular, 40/62 samples were positive by dd
PCR
(25 PB+/BM+; 9 PB+/BM−; 6 PB−/BM+), with 34/40 (85%) identified by the study of
PB
only. In conclusion, in localized
FL
, dd
PCR
is a promising tool for monitoring minimal residual disease (MRD) that is at least comparable to
RQ
‐
PCR
and potentially more accurate.
PB
is a suitable source for serial
BCL
2/
IGH
MRD
assessments, regardless of the methodology utilized.</description><identifier>ISSN: 0007-1048</identifier><identifier>EISSN: 1365-2141</identifier><identifier>DOI: 10.1111/bjh.14616</identifier><language>eng</language><ispartof>British journal of haematology, 2017-05, Vol.177 (4), p.588-596</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c746-78e73ef2a60a98deecf94ac1652b1be392a5ef85cc9d4f7a5cf95557267314383</citedby><cites>FETCH-LOGICAL-c746-78e73ef2a60a98deecf94ac1652b1be392a5ef85cc9d4f7a5cf95557267314383</cites><orcidid>0000-0002-2773-5483</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Cavalli, Marzia</creatorcontrib><creatorcontrib>De Novi, Lucia Anna</creatorcontrib><creatorcontrib>Della Starza, Irene</creatorcontrib><creatorcontrib>Cappelli, Luca Vincenzo</creatorcontrib><creatorcontrib>Nunes, Vittorio</creatorcontrib><creatorcontrib>Pulsoni, Alessandro</creatorcontrib><creatorcontrib>Del Giudice, Ilaria</creatorcontrib><creatorcontrib>Guarini, Anna</creatorcontrib><creatorcontrib>Foà, Robin</creatorcontrib><title>Comparative analysis between RQ ‐ PCR and digital droplet PCR of BCL 2/ IGH gene rearrangement in the peripheral blood and bone marrow of early stage follicular lymphoma</title><title>British journal of haematology</title><description>BCL
2/
IGH
rearrangements were analysed by polymerase chain reaction (
PCR
) at diagnosis in paired peripheral blood (
PB
) and bone marrow (
BM
) samples from 67 patients with stage I/
II
follicular lymphoma (
FL
). Real time quantitative
PCR
(
RQ
‐
PCR
) and digital droplet
PCR
(dd
PCR
) were performed in cases with a major breakpoint region (MBR+) at diagnosis and after localized radiotherapy and rituximab administration in order to investigate the applicability of dd
PCR
. The overall dd
PCR
/
RQ
‐
PCR
concordance was 81·9% (113/138 samples) and 97·5% in the 40/138 with quantifiable disease (
RQ
‐
PCR
≥10
−5
). At baseline, dd
PCR
allowed the recovery of a MBR+ marker in 8/18 (44·4%) samples that resulted MBR‐negative/minor cluster region‐negative/minor
BCL2
‐negative by qualitative
PCR
. Moreover, the tumour burden at diagnosis significantly predicted progression‐free survival (
PSF
) only when quantified by dd
PCR
. Paired
PB
and
BM
samples analysis demonstrated a high concordance in the detection of
BCL
2/IGH+
cells by qualitative and quantitative methods; in particular, 40/62 samples were positive by dd
PCR
(25 PB+/BM+; 9 PB+/BM−; 6 PB−/BM+), with 34/40 (85%) identified by the study of
PB
only. In conclusion, in localized
FL
, dd
PCR
is a promising tool for monitoring minimal residual disease (MRD) that is at least comparable to
RQ
‐
PCR
and potentially more accurate.
PB
is a suitable source for serial
BCL
2/
IGH
MRD
assessments, regardless of the methodology utilized.</description><issn>0007-1048</issn><issn>1365-2141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNotkE1OwzAQhS0EEqWw4AazZRFqx3F-lhBBW6kSUHUfOck4SeXEkROosuMI3INbcRLcwmxGmvfep9Ej5JbRe-Zmke_rexaELDwjM8ZD4fksYOdkRimNPEaD-JJcDcOeUsapYDPynZq2l1aOzQeC7KSehmaAHMcDYgfbN_j5_ILXdOu0EsqmakapobSm1zie7kbBY7oBfwHr5Qoq7BAsSmtlV2GL3QhNB2ON0KNt-hqti-famPIEzI2zt85tDkeQy-kJhlFWCMpo3RTvWlrQU9vXppXX5EJJPeDN_56T3fPTLl15m5flOn3YeEUUhF4UY8RR-TKkMolLxEIlgSxYKPyc5cgTXwpUsSiKpAxUJIXThRCRH0acBTzmc3L3hy2sGQaLKutt456cMkazY8mZKzk7lcx_AZJFcgw</recordid><startdate>201705</startdate><enddate>201705</enddate><creator>Cavalli, Marzia</creator><creator>De Novi, Lucia Anna</creator><creator>Della Starza, Irene</creator><creator>Cappelli, Luca Vincenzo</creator><creator>Nunes, Vittorio</creator><creator>Pulsoni, Alessandro</creator><creator>Del Giudice, Ilaria</creator><creator>Guarini, Anna</creator><creator>Foà, Robin</creator><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-2773-5483</orcidid></search><sort><creationdate>201705</creationdate><title>Comparative analysis between RQ ‐ PCR and digital droplet PCR of BCL 2/ IGH gene rearrangement in the peripheral blood and bone marrow of early stage follicular lymphoma</title><author>Cavalli, Marzia ; De Novi, Lucia Anna ; Della Starza, Irene ; Cappelli, Luca Vincenzo ; Nunes, Vittorio ; Pulsoni, Alessandro ; Del Giudice, Ilaria ; Guarini, Anna ; Foà, Robin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c746-78e73ef2a60a98deecf94ac1652b1be392a5ef85cc9d4f7a5cf95557267314383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cavalli, Marzia</creatorcontrib><creatorcontrib>De Novi, Lucia Anna</creatorcontrib><creatorcontrib>Della Starza, Irene</creatorcontrib><creatorcontrib>Cappelli, Luca Vincenzo</creatorcontrib><creatorcontrib>Nunes, Vittorio</creatorcontrib><creatorcontrib>Pulsoni, Alessandro</creatorcontrib><creatorcontrib>Del Giudice, Ilaria</creatorcontrib><creatorcontrib>Guarini, Anna</creatorcontrib><creatorcontrib>Foà, Robin</creatorcontrib><collection>CrossRef</collection><jtitle>British journal of haematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cavalli, Marzia</au><au>De Novi, Lucia Anna</au><au>Della Starza, Irene</au><au>Cappelli, Luca Vincenzo</au><au>Nunes, Vittorio</au><au>Pulsoni, Alessandro</au><au>Del Giudice, Ilaria</au><au>Guarini, Anna</au><au>Foà, Robin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative analysis between RQ ‐ PCR and digital droplet PCR of BCL 2/ IGH gene rearrangement in the peripheral blood and bone marrow of early stage follicular lymphoma</atitle><jtitle>British journal of haematology</jtitle><date>2017-05</date><risdate>2017</risdate><volume>177</volume><issue>4</issue><spage>588</spage><epage>596</epage><pages>588-596</pages><issn>0007-1048</issn><eissn>1365-2141</eissn><abstract>BCL
2/
IGH
rearrangements were analysed by polymerase chain reaction (
PCR
) at diagnosis in paired peripheral blood (
PB
) and bone marrow (
BM
) samples from 67 patients with stage I/
II
follicular lymphoma (
FL
). Real time quantitative
PCR
(
RQ
‐
PCR
) and digital droplet
PCR
(dd
PCR
) were performed in cases with a major breakpoint region (MBR+) at diagnosis and after localized radiotherapy and rituximab administration in order to investigate the applicability of dd
PCR
. The overall dd
PCR
/
RQ
‐
PCR
concordance was 81·9% (113/138 samples) and 97·5% in the 40/138 with quantifiable disease (
RQ
‐
PCR
≥10
−5
). At baseline, dd
PCR
allowed the recovery of a MBR+ marker in 8/18 (44·4%) samples that resulted MBR‐negative/minor cluster region‐negative/minor
BCL2
‐negative by qualitative
PCR
. Moreover, the tumour burden at diagnosis significantly predicted progression‐free survival (
PSF
) only when quantified by dd
PCR
. Paired
PB
and
BM
samples analysis demonstrated a high concordance in the detection of
BCL
2/IGH+
cells by qualitative and quantitative methods; in particular, 40/62 samples were positive by dd
PCR
(25 PB+/BM+; 9 PB+/BM−; 6 PB−/BM+), with 34/40 (85%) identified by the study of
PB
only. In conclusion, in localized
FL
, dd
PCR
is a promising tool for monitoring minimal residual disease (MRD) that is at least comparable to
RQ
‐
PCR
and potentially more accurate.
PB
is a suitable source for serial
BCL
2/
IGH
MRD
assessments, regardless of the methodology utilized.</abstract><doi>10.1111/bjh.14616</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-2773-5483</orcidid></addata></record> |
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title | Comparative analysis between RQ ‐ PCR and digital droplet PCR of BCL 2/ IGH gene rearrangement in the peripheral blood and bone marrow of early stage follicular lymphoma |
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