Fibroblast growth factor 2 ( FGF 2) is present in human spermatozoa and is related with sperm motility. The use of recombinant FGF 2 to improve motile sperm recovery

Fibroblast growth factor 2 ( FGF 2) is present in human spermatozoa and is related with sperm motility. The use of recombinant FGF 2 to improve motile sperm recovery

Fibroblast growth factors ( FGF s) and their receptors ( FGFR s) regulate several functions of somatic cells. In a previous work, we reported FGFR expression in human spermatozoa and their involvement in motility. This study aimed to evaluate the presence and localization of fibroblast growth factor...

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Veröffentlicht in:Andrology (Oxford) 2017-09, Vol.5 (5), p.990-998
Hauptverfasser: Garbarino Azúa, D. J., Saucedo, L., Giordana, S., Magri, M. L., Buffone, M. G., Neuspiller, F., Vazquez‐Levin, M. H., Marín‐Briggiler, C. I.
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container_issue 5
container_start_page 990
container_title Andrology (Oxford)
container_volume 5
creator Garbarino Azúa, D. J.
Saucedo, L.
Giordana, S.
Magri, M. L.
Buffone, M. G.
Neuspiller, F.
Vazquez‐Levin, M. H.
Marín‐Briggiler, C. I.
description Fibroblast growth factors ( FGF s) and their receptors ( FGFR s) regulate several functions of somatic cells. In a previous work, we reported FGFR expression in human spermatozoa and their involvement in motility. This study aimed to evaluate the presence and localization of fibroblast growth factor 2 ( FGF 2) in human spermatozoa, to determine the relationship of FGF 2 levels with conventional semen parameters and to assess the effect of recombinant FGF 2 ( rFGF 2) on sperm recovery in a selection procedure. Western immunoblotting analysis using an antibody against FGF 2 revealed an 18‐ kD a band in sperm protein extracts. The protein was immunolocalized in the sperm flagellum and acrosomal region, as well as in all germ cells. Sperm FGF 2 levels, assessed by flow cytometry, showed a positive ( p  
doi_str_mv 10.1111/andr.12398
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The use of recombinant FGF 2 to improve motile sperm recovery</title><source>Wiley Free Content</source><source>Wiley Online Library All Journals</source><creator>Garbarino Azúa, D. J. ; Saucedo, L. ; Giordana, S. ; Magri, M. L. ; Buffone, M. G. ; Neuspiller, F. ; Vazquez‐Levin, M. H. ; Marín‐Briggiler, C. I.</creator><creatorcontrib>Garbarino Azúa, D. J. ; Saucedo, L. ; Giordana, S. ; Magri, M. L. ; Buffone, M. G. ; Neuspiller, F. ; Vazquez‐Levin, M. H. ; Marín‐Briggiler, C. I.</creatorcontrib><description>Fibroblast growth factors ( FGF s) and their receptors ( FGFR s) regulate several functions of somatic cells. In a previous work, we reported FGFR expression in human spermatozoa and their involvement in motility. This study aimed to evaluate the presence and localization of fibroblast growth factor 2 ( FGF 2) in human spermatozoa, to determine the relationship of FGF 2 levels with conventional semen parameters and to assess the effect of recombinant FGF 2 ( rFGF 2) on sperm recovery in a selection procedure. Western immunoblotting analysis using an antibody against FGF 2 revealed an 18‐ kD a band in sperm protein extracts. The protein was immunolocalized in the sperm flagellum and acrosomal region, as well as in all germ cells. Sperm FGF 2 levels, assessed by flow cytometry, showed a positive ( p  &lt; 0.05) correlation with sperm concentration, motility, total sperm number and total motile cells per ejaculate. Moreover, samples with abnormal motility depicted diminished ( p  &lt; 0.01) FGF 2 levels compared to those with normal motility. Spermatozoa exposed to rFGF 2 bound the protein, exhibited higher ( p  &lt; 0.05) total and motile sperm recoveries, and increased ( p  &lt; 0.01) kinematic parameters after the swim‐up. 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title Fibroblast growth factor 2 ( FGF 2) is present in human spermatozoa and is related with sperm motility. The use of recombinant FGF 2 to improve motile sperm recovery
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