Use of RN ase H‐dependent PCR for discrimination and detection of closely related species from environmental DNA

Species‐specific, probe‐based quantitative PCR (qPCR) assays are now commonly used to detect aquatic species from environmental DNA . However, probe‐based qPCR alone does not always provide the specificity needed to distinguish closely related, congeneric species, which may result in amplification o...

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Veröffentlicht in:	Methods in ecology and evolution 2019-07, Vol.10 (7), p.1091-1096
Hauptverfasser:	Rodgers, Torrey W., Olson, John R., Mock, Karen E.
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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