The Quantification of Representative Sequences pipeline for amplicon sequencing: case study on within‐population ITS 1 sequence variation in a microparasite infecting D aphnia
Next generation sequencing ( NGS ) platforms are replacing traditional molecular biology protocols like cloning and Sanger sequencing. However, accuracy of NGS platforms has rarely been measured when quantifying relative frequencies of genotypes or taxa within populations. Here we developed a new bi...
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Veröffentlicht in: | Molecular ecology resources 2015-11, Vol.15 (6), p.1385-1395 |
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Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
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