In vitro effects of oil's fatty acids on T cell function in gestational diabetic pregnant women and their newborns 油类脂肪酸在体外对妊娠期糖尿病孕妇及其新生儿的T细胞功能的影响

Background The aim of this investigation was to determine the in vitro effects of linseed, olive and Nigel oils on T cell proliferation and function in gestational diabetes. Methods Blood samples were collected from 40 control healthy and 32 gestational diabetic mothers and their newborns. Peripheral blood lymphocytes were isolated using a density gradient of Ficoll. T cell proliferation, interleukin‐2 and ‐4 (IL‐2, IL‐4) secretion, fatty acid composition and intracellular oxidative status were investigated. Results Mitogen (Concanavalin A) stimulated lymphocyte proliferation, IL‐2 secretion, intracellular reduced glutathione levels, superoxide dismutase (SOD) and catalase activities were lower while intracellular malondialdehyde (MDA) and carbonyl proteins were higher in diabetic mothers and in their newborns as compared to their respective controls. Linseed oil induced a reduction in T‐lymphocyte proliferation and IL‐2 production, and alpha linolenic acid membrane enrichment in both diabetic and control groups. In the presence of Nigel oil, T‐lymphocyte proliferation and IL‐2 secretion, phospholipid linoleic and oleic acids were enhanced. Olive oil had no effect on lymphocyte proliferation in all groups. Linseed, olive and Nigel oils induced an increase in T cell levels of reduced glutathione levels and in activities of catalase and SOD with a concomitant decrease in MDA and carbonyl protein contents. Conclusion Linseed, olive and Nigel oils had beneficial effects on T cell functions in gestational diabetes. 摘要 背景： 本研究的目的是在妊娠期糖尿病孕妇中调查亚麻油、橄榄油与奈吉尔油在体外对T细胞增殖以及功能的影响。 方法： 收集40名对照的健康母亲与32名妊娠期糖尿病母亲及其新生儿的血样。利用聚蔗糖密度梯度来分离外周血淋巴细胞。调查了T细胞的增殖情况、白细胞介素‐2与白细胞介素‐4（IL‐2，IL‐4）的分泌量、脂肪酸成份以及细胞内的氧化状态。 结果： 糖尿病母亲及其新生儿与她们各自的对照组相比，分裂素（刀豆球蛋白A）刺激的淋巴细胞增殖、IL‐2分泌、细胞内还原型谷胱甘肽水平、超氧化物歧化酶以及过氧化氢酶活性都更低，而细胞内的丙二醛与羰基蛋白质水平更高。在糖尿病组与对照组中，亚麻油诱导后T淋巴细胞增殖、IL‐2生成、以及富集α亚麻酸的细胞膜都减少了。在存在奈吉尔油的环境中，T‐淋巴细胞增殖、IL‐2分泌、磷脂亚油酸以及油酸都增加了。在所有分组中橄榄油对淋巴细胞的增殖都没有影响。亚麻油、橄榄油与奈吉尔油可以导致T细胞中的还原型谷胱甘肽水平以及过氧化氢酶与超氧化物歧化酶的活性增加，同时伴随着丙二醛与羰基蛋白质含量减少。 结论： 亚麻油、橄榄油与奈吉尔油对妊娠期糖尿病孕妇的T细胞功能有益。