Secretory production of an FAD cofactor‐containing cytosolic enzyme (sorbitol–xylitol oxidase from S treptomyces coelicolor ) using the twin‐arginine translocation ( Tat ) pathway of C orynebacterium glutamicum

Carbohydrate oxidases are biotechnologically interesting enzymes that require a tightly or covalently bound cofactor for activity. Using the industrial workhorse C orynebacterium glutamicum as the expression host, successful secretion of a normally cytosolic FAD cofactor‐containing sorbitol–xylitol oxidase from S treptomyces coelicolor was achieved by using the twin‐arginine translocation ( Tat ) protein export machinery for protein translocation across the cytoplasmic membrane. Our results demonstrate for the first time that, also for cofactor‐containing proteins, a secretory production strategy is a feasible and promising alternative to conventional intracellular expression strategies.