Flexible ITO Electrode With Gold Nanostructures for Femtomolar DNA Hybridization Detection
In this article, we report detection of DNA hybridization using a gold nanostructure coated flexible indium tin oxide (ITO) working electrode, aiming at exploring alternate materials for developing low-cost light-weight sensing platforms in the future. Herein, gold nanostructures were electrodeposit...
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| Veröffentlicht in: | IEEE sensors letters 2018-12, Vol.2 (4), p.1-4 |
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| creator | Tripathy, Suryasnata Joseph, Jose Vanjari, Siva Rama Krishna Rao, A.V.S.S. Narayana Singh, Shiv Govind |
| description | In this article, we report detection of DNA hybridization using a gold nanostructure coated flexible indium tin oxide (ITO) working electrode, aiming at exploring alternate materials for developing low-cost light-weight sensing platforms in the future. Herein, gold nanostructures were electrodeposited onto the flexible ITO substrate with HAuCl 4 precursor solution. To prepare the bioelectrode for DNA sensing, thiol-modified single-stranded DNA probes specific to the BRCA1 gene were attached to the nanostructured gold surface via self-assembly. When subjected to hybridization detection in a wide concentration range of 1 fM-1 μM, the bioelectrode resulted in a limiting detection of 0.255 fM (calculated with 3.3σ method), with a sensitivity of 10.94 (μA/mol)/cm 2 . In order to estimate the efficiency of the sensor, we have performed interference analysis in the presence of a noncomplementary target DNA and human serum albumin. Furthermore, the reproducibility of the biosensor was verified using a blind testing protocol. |
| doi_str_mv | 10.1109/LSENS.2018.2874232 |
| format | Article |
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Narayana ; Singh, Shiv Govind</creator><creatorcontrib>Tripathy, Suryasnata ; Joseph, Jose ; Vanjari, Siva Rama Krishna ; Rao, A.V.S.S. Narayana ; Singh, Shiv Govind</creatorcontrib><description>In this article, we report detection of DNA hybridization using a gold nanostructure coated flexible indium tin oxide (ITO) working electrode, aiming at exploring alternate materials for developing low-cost light-weight sensing platforms in the future. Herein, gold nanostructures were electrodeposited onto the flexible ITO substrate with HAuCl 4 precursor solution. To prepare the bioelectrode for DNA sensing, thiol-modified single-stranded DNA probes specific to the BRCA1 gene were attached to the nanostructured gold surface via self-assembly. When subjected to hybridization detection in a wide concentration range of 1 fM-1 μM, the bioelectrode resulted in a limiting detection of 0.255 fM (calculated with 3.3σ method), with a sensitivity of 10.94 (μA/mol)/cm 2 . In order to estimate the efficiency of the sensor, we have performed interference analysis in the presence of a noncomplementary target DNA and human serum albumin. 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(IEEE) 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c225t-451d81c92e7cfdbd0210601d03cbd7ce9058f8e123ff239bbe509edc4c1df123</citedby><cites>FETCH-LOGICAL-c225t-451d81c92e7cfdbd0210601d03cbd7ce9058f8e123ff239bbe509edc4c1df123</cites><orcidid>0000-0002-9159-4224 ; 0000-0001-7319-879X ; 0000-0002-5621-7044</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://ieeexplore.ieee.org/document/8486687$$EHTML$$P50$$Gieee$$H</linktohtml><link.rule.ids>314,780,784,796,27924,27925,54758</link.rule.ids><linktorsrc>$$Uhttps://ieeexplore.ieee.org/document/8486687$$EView_record_in_IEEE$$FView_record_in_$$GIEEE</linktorsrc></links><search><creatorcontrib>Tripathy, Suryasnata</creatorcontrib><creatorcontrib>Joseph, Jose</creatorcontrib><creatorcontrib>Vanjari, Siva Rama Krishna</creatorcontrib><creatorcontrib>Rao, A.V.S.S. Narayana</creatorcontrib><creatorcontrib>Singh, Shiv Govind</creatorcontrib><title>Flexible ITO Electrode With Gold Nanostructures for Femtomolar DNA Hybridization Detection</title><title>IEEE sensors letters</title><addtitle>LSENS</addtitle><description>In this article, we report detection of DNA hybridization using a gold nanostructure coated flexible indium tin oxide (ITO) working electrode, aiming at exploring alternate materials for developing low-cost light-weight sensing platforms in the future. Herein, gold nanostructures were electrodeposited onto the flexible ITO substrate with HAuCl 4 precursor solution. To prepare the bioelectrode for DNA sensing, thiol-modified single-stranded DNA probes specific to the BRCA1 gene were attached to the nanostructured gold surface via self-assembly. When subjected to hybridization detection in a wide concentration range of 1 fM-1 μM, the bioelectrode resulted in a limiting detection of 0.255 fM (calculated with 3.3σ method), with a sensitivity of 10.94 (μA/mol)/cm 2 . In order to estimate the efficiency of the sensor, we have performed interference analysis in the presence of a noncomplementary target DNA and human serum albumin. Furthermore, the reproducibility of the biosensor was verified using a blind testing protocol.</description><subject>Biosensors</subject><subject>Chemical and biological sensors</subject><subject>Coated electrodes</subject><subject>Deoxyribonucleic acid</subject><subject>differential pulse voltammetry (DPV)</subject><subject>DNA</subject><subject>DNA hybridization</subject><subject>electrodeposition</subject><subject>flexible substrate</subject><subject>Gold</subject><subject>Hybridization</subject><subject>Indium tin oxides</subject><subject>Nanostructure</subject><subject>Self-assembly</subject><subject>Sensors</subject><subject>Serum albumin</subject><subject>Substrates</subject><subject>Test procedures</subject><subject>Weight reduction</subject><issn>2475-1472</issn><issn>2475-1472</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>RIE</sourceid><recordid>eNpNkMtKAzEUhoMoWLQvoJuA66nJmZlOZll6h9IuWhDchJnkBKdMm5qkYH16U1vE1fk5_Bf4CHnirMc5K18X6_Fy3QPGRQ9EkUEKN6QDWZEnPCvg9p--J13vt4xFKxQsZR3yPmnxq6lbpPPNio5bVMFZjfStCR90altNl9Xe-uCOKhwdemqsoxPcBbuzbeXoaDmgs1PtGt18V6GxezrCEEuieiR3pmo9dq_3gWwm481wlixW0_lwsEgUQB6SLOdacFUCFsroWjPgrM-4ZqmqdaGwZLkwAjmkxkBa1jXmrEStMsW1id8H8nKpPTj7eUQf5NYe3T4uSoBSpGmeiSK64OJSznrv0MiDa3aVO0nO5Jmi_KUozxTllWIMPV9CDSL-BUQm-v1Y-QNVoW5m</recordid><startdate>20181201</startdate><enddate>20181201</enddate><creator>Tripathy, Suryasnata</creator><creator>Joseph, Jose</creator><creator>Vanjari, Siva Rama Krishna</creator><creator>Rao, A.V.S.S. Narayana</creator><creator>Singh, Shiv Govind</creator><general>IEEE</general><general>The Institute of Electrical and Electronics Engineers, Inc. (IEEE)</general><scope>97E</scope><scope>RIA</scope><scope>RIE</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>8FD</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0002-9159-4224</orcidid><orcidid>https://orcid.org/0000-0001-7319-879X</orcidid><orcidid>https://orcid.org/0000-0002-5621-7044</orcidid></search><sort><creationdate>20181201</creationdate><title>Flexible ITO Electrode With Gold Nanostructures for Femtomolar DNA Hybridization Detection</title><author>Tripathy, Suryasnata ; Joseph, Jose ; Vanjari, Siva Rama Krishna ; Rao, A.V.S.S. Narayana ; Singh, Shiv Govind</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c225t-451d81c92e7cfdbd0210601d03cbd7ce9058f8e123ff239bbe509edc4c1df123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Biosensors</topic><topic>Chemical and biological sensors</topic><topic>Coated electrodes</topic><topic>Deoxyribonucleic acid</topic><topic>differential pulse voltammetry (DPV)</topic><topic>DNA</topic><topic>DNA hybridization</topic><topic>electrodeposition</topic><topic>flexible substrate</topic><topic>Gold</topic><topic>Hybridization</topic><topic>Indium tin oxides</topic><topic>Nanostructure</topic><topic>Self-assembly</topic><topic>Sensors</topic><topic>Serum albumin</topic><topic>Substrates</topic><topic>Test procedures</topic><topic>Weight reduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tripathy, Suryasnata</creatorcontrib><creatorcontrib>Joseph, Jose</creatorcontrib><creatorcontrib>Vanjari, Siva Rama Krishna</creatorcontrib><creatorcontrib>Rao, A.V.S.S. 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| subjects | Biosensors Chemical and biological sensors Coated electrodes Deoxyribonucleic acid differential pulse voltammetry (DPV) DNA DNA hybridization electrodeposition flexible substrate Gold Hybridization Indium tin oxides Nanostructure Self-assembly Sensors Serum albumin Substrates Test procedures Weight reduction |
| title | Flexible ITO Electrode With Gold Nanostructures for Femtomolar DNA Hybridization Detection |
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