Background-Free Volumetric Two-Photon Microscopy by Side-Lobes-Cancelled Bessel Beam
We have demonstrated the background-free volumetric two-photon microscopy by cancelling the side lobes of the fundamental 0th-order Bessel beam. The cancellation is based on the fact that the lateral ring patterns of the 3rd-order Bessel beam are well-matched with the side lobes of the 0th-order Bes...
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| Veröffentlicht in: | IEEE journal of selected topics in quantum electronics 2021-07, Vol.27 (4), p.1-7 |
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| container_title | IEEE journal of selected topics in quantum electronics |
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| creator | He, Hongsen Ren, Yu-Xuan Chan, Ryan K. Y. So, W. L. Fok, Hiu Ka Lai, Cora S. W. Tsia, Kevin K. Wong, Kenneth K. Y. |
| description | We have demonstrated the background-free volumetric two-photon microscopy by cancelling the side lobes of the fundamental 0th-order Bessel beam. The cancellation is based on the fact that the lateral ring patterns of the 3rd-order Bessel beam are well-matched with the side lobes of the 0th-order Bessel beam, so that the image obtained by the 3rd-order Bessel beam is capable to be subtracted as the background of the 0th-order-Bessel-beam-scanned image. We investigated the intensity of the imaging background introduced by the Bessel side lobes under different focusing conditions both in simulations and experiments. The performance of the side-lobes cancellation is showcased on a 50-μm-thick mouse brain slice, and the contrast ratio is enhanced by 2.7 dB for the neurons. This background cancellation technique provides a simple and versatile tool for enhancing the contrast ratio in the two-photon volumetric imaging, especially critical in high-NA cases when a better lateral resolution is pursued. |
| doi_str_mv | 10.1109/JSTQE.2021.3054892 |
| format | Article |
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Y. ; So, W. L. ; Fok, Hiu Ka ; Lai, Cora S. W. ; Tsia, Kevin K. ; Wong, Kenneth K. Y.</creator><creatorcontrib>He, Hongsen ; Ren, Yu-Xuan ; Chan, Ryan K. Y. ; So, W. L. ; Fok, Hiu Ka ; Lai, Cora S. W. ; Tsia, Kevin K. ; Wong, Kenneth K. Y.</creatorcontrib><description>We have demonstrated the background-free volumetric two-photon microscopy by cancelling the side lobes of the fundamental 0th-order Bessel beam. The cancellation is based on the fact that the lateral ring patterns of the 3rd-order Bessel beam are well-matched with the side lobes of the 0th-order Bessel beam, so that the image obtained by the 3rd-order Bessel beam is capable to be subtracted as the background of the 0th-order-Bessel-beam-scanned image. We investigated the intensity of the imaging background introduced by the Bessel side lobes under different focusing conditions both in simulations and experiments. The performance of the side-lobes cancellation is showcased on a 50-μm-thick mouse brain slice, and the contrast ratio is enhanced by 2.7 dB for the neurons. This background cancellation technique provides a simple and versatile tool for enhancing the contrast ratio in the two-photon volumetric imaging, especially critical in high-NA cases when a better lateral resolution is pursued.</description><identifier>ISSN: 1077-260X</identifier><identifier>EISSN: 1558-4542</identifier><identifier>DOI: 10.1109/JSTQE.2021.3054892</identifier><identifier>CODEN: IJSQEN</identifier><language>eng</language><publisher>New York: IEEE</publisher><subject>Beams (radiation) ; Bessel beam ; Cancellation ; contrast enhancement ; Deconvolution ; Fluorescence ; Imaging ; Laser beams ; Laser excitation ; Lobes ; Microscopy ; Photons ; side lobes ; Sidelobes ; two-photon microscopy ; volumetric imaging</subject><ispartof>IEEE journal of selected topics in quantum electronics, 2021-07, Vol.27 (4), p.1-7</ispartof><rights>Copyright The Institute of Electrical and Electronics Engineers, Inc. (IEEE) 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-97ba2803efba6bc74e23c98c39e277ba24228322b2c2bfcf42fd408c3ae5f823</citedby><cites>FETCH-LOGICAL-c344t-97ba2803efba6bc74e23c98c39e277ba24228322b2c2bfcf42fd408c3ae5f823</cites><orcidid>0000-0002-5298-8992 ; 0000-0003-3668-3539</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://ieeexplore.ieee.org/document/9340242$$EHTML$$P50$$Gieee$$H</linktohtml><link.rule.ids>314,780,784,796,27924,27925,54758</link.rule.ids><linktorsrc>$$Uhttps://ieeexplore.ieee.org/document/9340242$$EView_record_in_IEEE$$FView_record_in_$$GIEEE</linktorsrc></links><search><creatorcontrib>He, Hongsen</creatorcontrib><creatorcontrib>Ren, Yu-Xuan</creatorcontrib><creatorcontrib>Chan, Ryan K. Y.</creatorcontrib><creatorcontrib>So, W. 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We investigated the intensity of the imaging background introduced by the Bessel side lobes under different focusing conditions both in simulations and experiments. The performance of the side-lobes cancellation is showcased on a 50-μm-thick mouse brain slice, and the contrast ratio is enhanced by 2.7 dB for the neurons. This background cancellation technique provides a simple and versatile tool for enhancing the contrast ratio in the two-photon volumetric imaging, especially critical in high-NA cases when a better lateral resolution is pursued.</description><subject>Beams (radiation)</subject><subject>Bessel beam</subject><subject>Cancellation</subject><subject>contrast enhancement</subject><subject>Deconvolution</subject><subject>Fluorescence</subject><subject>Imaging</subject><subject>Laser beams</subject><subject>Laser excitation</subject><subject>Lobes</subject><subject>Microscopy</subject><subject>Photons</subject><subject>side lobes</subject><subject>Sidelobes</subject><subject>two-photon microscopy</subject><subject>volumetric imaging</subject><issn>1077-260X</issn><issn>1558-4542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>RIE</sourceid><recordid>eNo9kMtOwzAQRS0EEqXwA7CJxNrFHjuNs6RVy0NFgBohdpbjTCAljYudCPXvSWnF6o40987jEHLJ2Yhzlt48LrPX2QgY8JFgsVQpHJEBj2NFZSzhuK9ZklAYs_dTchbCijGmpGIDkk2M_frwrmsKOveI0ZuruzW2vrJR9uPoy6drXRM9Vda7YN1mG-XbaFkVSBcux0CnprFY11hEEwwB617M-pyclKYOeHHQIcnms2x6TxfPdw_T2wW1QsqWpkluQDGBZW7GuU0kgrCpsiJFSHY9CaAEQA4W8tKWEspCsr5vMC4ViCG53o_dePfdYWj1ynW-6TdqkClTjPNx2rtg79p9EDyWeuOrtfFbzZnewdN_8PQOnj7A60NX-1CFiP-BVEjWHyV-AXlRazQ</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>He, Hongsen</creator><creator>Ren, Yu-Xuan</creator><creator>Chan, Ryan K. 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L.</creatorcontrib><creatorcontrib>Fok, Hiu Ka</creatorcontrib><creatorcontrib>Lai, Cora S. W.</creatorcontrib><creatorcontrib>Tsia, Kevin K.</creatorcontrib><creatorcontrib>Wong, Kenneth K. Y.</creatorcontrib><collection>IEEE All-Society Periodicals Package (ASPP) 2005-present</collection><collection>IEEE All-Society Periodicals Package (ASPP) 1998-Present</collection><collection>IEEE Electronic Library (IEL)</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>IEEE journal of selected topics in quantum electronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>He, Hongsen</au><au>Ren, Yu-Xuan</au><au>Chan, Ryan K. Y.</au><au>So, W. L.</au><au>Fok, Hiu Ka</au><au>Lai, Cora S. W.</au><au>Tsia, Kevin K.</au><au>Wong, Kenneth K. Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Background-Free Volumetric Two-Photon Microscopy by Side-Lobes-Cancelled Bessel Beam</atitle><jtitle>IEEE journal of selected topics in quantum electronics</jtitle><stitle>JSTQE</stitle><date>2021-07-01</date><risdate>2021</risdate><volume>27</volume><issue>4</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>1077-260X</issn><eissn>1558-4542</eissn><coden>IJSQEN</coden><abstract>We have demonstrated the background-free volumetric two-photon microscopy by cancelling the side lobes of the fundamental 0th-order Bessel beam. The cancellation is based on the fact that the lateral ring patterns of the 3rd-order Bessel beam are well-matched with the side lobes of the 0th-order Bessel beam, so that the image obtained by the 3rd-order Bessel beam is capable to be subtracted as the background of the 0th-order-Bessel-beam-scanned image. We investigated the intensity of the imaging background introduced by the Bessel side lobes under different focusing conditions both in simulations and experiments. The performance of the side-lobes cancellation is showcased on a 50-μm-thick mouse brain slice, and the contrast ratio is enhanced by 2.7 dB for the neurons. This background cancellation technique provides a simple and versatile tool for enhancing the contrast ratio in the two-photon volumetric imaging, especially critical in high-NA cases when a better lateral resolution is pursued.</abstract><cop>New York</cop><pub>IEEE</pub><doi>10.1109/JSTQE.2021.3054892</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-5298-8992</orcidid><orcidid>https://orcid.org/0000-0003-3668-3539</orcidid></addata></record> |
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| subjects | Beams (radiation) Bessel beam Cancellation contrast enhancement Deconvolution Fluorescence Imaging Laser beams Laser excitation Lobes Microscopy Photons side lobes Sidelobes two-photon microscopy volumetric imaging |
| title | Background-Free Volumetric Two-Photon Microscopy by Side-Lobes-Cancelled Bessel Beam |
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