Plastic-embedded protein crystals

Rapid vitrification followed by the replacement of the vitrified water by a solvent (freeze substitution) and then resin is a widely used procedure for preparing biological samples for electron microscopy. The resulting plastic‐embedded samples permit convenient room‐temperature sectioning (microto...

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Veröffentlicht in:	Journal of synchrotron radiation 2007-01, Vol.14 (1), p.128-132
Hauptverfasser:	Ravelli, Raimond B. G., Haselmann-Weiss, Uta, McGeehan, John E., McCarthy, Andrew A., Marquez, Josan A., Antony, Claude, Frangakis, Achilleas S., Stranzl, Gudrun
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrylic Resins - chemistry Acrylic Resins - radiation effects Coated Materials, Biocompatible - chemistry Coated Materials, Biocompatible - radiation effects Computer Simulation Crystallization - methods Dose-Response Relationship, Radiation freeze substitution Models, Chemical Models, Molecular Muramidase - chemistry Muramidase - radiation effects Muramidase - ultrastructure Plastic Embedding - methods Protein Conformation - radiation effects Protein Denaturation - radiation effects radiation damage Radiation Dosage resin embedding Specimen Handling - methods X-Ray Diffraction - methods X-Rays
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