Functional characterization and expression analyses of the glucose-specific AtSTP9 monosaccharide transporter in pollen of Arabidopsis

A genomic clone and the corresponding cDNA of a new Arabidopsis monosaccharide transporter AtSTP9 were isolated. Transport analysis of the expressed protein in yeast showed that AtSTP9 is an energy-dependent, uncoupler-sensitive, high-affinity monosaccharide transporter with a Km for glucose in the...

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Veröffentlicht in:	Plant physiology (Bethesda) 2003-09, Vol.133 (1), p.182-190
Hauptverfasser:	Schneidereit, A, Scholz-Starke, J, Buttner, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana AtSTP9 gene Biological and medical sciences Cell Biology and Signal Transduction Cell physiology Cloning, Molecular Complementary DNA DNA, Bacterial - genetics DNA, Complementary - chemistry DNA, Complementary - genetics Flowers Fundamental and applied biological sciences. Psychology gametophytes gene expression Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Reporter - genetics Genes, Reporter - physiology Genomics glucose Glucose - metabolism insertional mutagenesis messenger RNA molecular cloning Molecular Sequence Data Monosaccharide Transport Proteins - genetics Monosaccharide Transport Proteins - metabolism Monosaccharides Mutagenesis, Insertional Mutation nucleotide sequences Plant physiology and development plant proteins Plants Plasma membrane and permeation Pollen Pollen - genetics Pollen - growth & development Pollen - metabolism pollen germination Pollen tubes Polymerase chain reaction promoter regions Promoter Regions, Genetic - genetics Reverse Transcriptase Polymerase Chain Reaction sequence alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Sugars tissue distribution transfer DNA transporters Yeasts
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