Functional characterization and expression analyses of the glucose-specific AtSTP9 monosaccharide transporter in pollen of Arabidopsis
A genomic clone and the corresponding cDNA of a new Arabidopsis monosaccharide transporter AtSTP9 were isolated. Transport analysis of the expressed protein in yeast showed that AtSTP9 is an energy-dependent, uncoupler-sensitive, high-affinity monosaccharide transporter with a Km for glucose in the...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2003-09, Vol.133 (1), p.182-190 |
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| creator | Schneidereit, A Scholz-Starke, J Buttner, M |
| description | A genomic clone and the corresponding cDNA of a new Arabidopsis monosaccharide transporter AtSTP9 were isolated. Transport analysis of the expressed protein in yeast showed that AtSTP9 is an energy-dependent, uncoupler-sensitive, high-affinity monosaccharide transporter with a Km for glucose in the micromolar range. In contrast to all previously characterized monosaccharide transporters, AtSTP9 shows an unusual specificity for glucose. Reverse transcriptase-polymerase chain reaction analyses revealed that AtSTP9 is exclusively expressed in flowers, and a more detailed approach using AtSTP9 promoter/reporter plants clearly showed that AtSTP9 expression is restricted to the male gametophyte. AtSTP9 expression is not found in other floral organs or vegetative tissues. Further localization on the cellular level using a specific antibody revealed that in contrast to the early accumulation of AtSTP9 transcripts in young pollen, the AtSTP9 protein is only found weakly in mature pollen but is most prominent in germinating pollen tubes. This preloading of pollen with mRNAs has been described for genes that are essential for pollen germination and/or pollen tube growth. The pollen-specific expression found for AtSTP9 is also observed for other sugar transporters and indicates that pollen development and germination require a highly regulated supply of sugars. |
| doi_str_mv | 10.1104/pp.103.026674 |
| format | Article |
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Transport analysis of the expressed protein in yeast showed that AtSTP9 is an energy-dependent, uncoupler-sensitive, high-affinity monosaccharide transporter with a Km for glucose in the micromolar range. In contrast to all previously characterized monosaccharide transporters, AtSTP9 shows an unusual specificity for glucose. Reverse transcriptase-polymerase chain reaction analyses revealed that AtSTP9 is exclusively expressed in flowers, and a more detailed approach using AtSTP9 promoter/reporter plants clearly showed that AtSTP9 expression is restricted to the male gametophyte. AtSTP9 expression is not found in other floral organs or vegetative tissues. Further localization on the cellular level using a specific antibody revealed that in contrast to the early accumulation of AtSTP9 transcripts in young pollen, the AtSTP9 protein is only found weakly in mature pollen but is most prominent in germinating pollen tubes. This preloading of pollen with mRNAs has been described for genes that are essential for pollen germination and/or pollen tube growth. The pollen-specific expression found for AtSTP9 is also observed for other sugar transporters and indicates that pollen development and germination require a highly regulated supply of sugars.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.103.026674</identifier><identifier>PMID: 12970485</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Biologists</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; AtSTP9 gene ; Biological and medical sciences ; Cell Biology and Signal Transduction ; Cell physiology ; Cloning, Molecular ; Complementary DNA ; DNA, Bacterial - genetics ; DNA, Complementary - chemistry ; DNA, Complementary - genetics ; Flowers ; Fundamental and applied biological sciences. Psychology ; gametophytes ; gene expression ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Plant ; Genes, Reporter - genetics ; Genes, Reporter - physiology ; Genomics ; glucose ; Glucose - metabolism ; insertional mutagenesis ; messenger RNA ; molecular cloning ; Molecular Sequence Data ; Monosaccharide Transport Proteins - genetics ; Monosaccharide Transport Proteins - metabolism ; Monosaccharides ; Mutagenesis, Insertional ; Mutation ; nucleotide sequences ; Plant physiology and development ; plant proteins ; Plants ; Plasma membrane and permeation ; Pollen ; Pollen - genetics ; Pollen - growth & development ; Pollen - metabolism ; pollen germination ; Pollen tubes ; Polymerase chain reaction ; promoter regions ; Promoter Regions, Genetic - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; sequence alignment ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Sugars ; tissue distribution ; transfer DNA ; transporters ; Yeasts</subject><ispartof>Plant physiology (Bethesda), 2003-09, Vol.133 (1), p.182-190</ispartof><rights>Copyright 2003 American Society of Plant Biologists</rights><rights>2003 INIST-CNRS</rights><rights>Copyright © 2003, The American Society for Plant Biologists 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-57c6f39a5db65507dce7bfa76bd3debdce611f7e0eb016e1fb379746f039ebf53</citedby><cites>FETCH-LOGICAL-c493t-57c6f39a5db65507dce7bfa76bd3debdce611f7e0eb016e1fb379746f039ebf53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4281328$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4281328$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15125735$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12970485$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schneidereit, A</creatorcontrib><creatorcontrib>Scholz-Starke, J</creatorcontrib><creatorcontrib>Buttner, M</creatorcontrib><title>Functional characterization and expression analyses of the glucose-specific AtSTP9 monosaccharide transporter in pollen of Arabidopsis</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>A genomic clone and the corresponding cDNA of a new Arabidopsis monosaccharide transporter AtSTP9 were isolated. Transport analysis of the expressed protein in yeast showed that AtSTP9 is an energy-dependent, uncoupler-sensitive, high-affinity monosaccharide transporter with a Km for glucose in the micromolar range. In contrast to all previously characterized monosaccharide transporters, AtSTP9 shows an unusual specificity for glucose. Reverse transcriptase-polymerase chain reaction analyses revealed that AtSTP9 is exclusively expressed in flowers, and a more detailed approach using AtSTP9 promoter/reporter plants clearly showed that AtSTP9 expression is restricted to the male gametophyte. AtSTP9 expression is not found in other floral organs or vegetative tissues. Further localization on the cellular level using a specific antibody revealed that in contrast to the early accumulation of AtSTP9 transcripts in young pollen, the AtSTP9 protein is only found weakly in mature pollen but is most prominent in germinating pollen tubes. This preloading of pollen with mRNAs has been described for genes that are essential for pollen germination and/or pollen tube growth. The pollen-specific expression found for AtSTP9 is also observed for other sugar transporters and indicates that pollen development and germination require a highly regulated supply of sugars.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>AtSTP9 gene</subject><subject>Biological and medical sciences</subject><subject>Cell Biology and Signal Transduction</subject><subject>Cell physiology</subject><subject>Cloning, Molecular</subject><subject>Complementary DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Complementary - genetics</subject><subject>Flowers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gametophytes</subject><subject>gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes, Reporter - genetics</subject><subject>Genes, Reporter - physiology</subject><subject>Genomics</subject><subject>glucose</subject><subject>Glucose - metabolism</subject><subject>insertional mutagenesis</subject><subject>messenger RNA</subject><subject>molecular cloning</subject><subject>Molecular Sequence Data</subject><subject>Monosaccharide Transport Proteins - genetics</subject><subject>Monosaccharide Transport Proteins - metabolism</subject><subject>Monosaccharides</subject><subject>Mutagenesis, Insertional</subject><subject>Mutation</subject><subject>nucleotide sequences</subject><subject>Plant physiology and development</subject><subject>plant proteins</subject><subject>Plants</subject><subject>Plasma membrane and permeation</subject><subject>Pollen</subject><subject>Pollen - genetics</subject><subject>Pollen - growth & development</subject><subject>Pollen - metabolism</subject><subject>pollen germination</subject><subject>Pollen tubes</subject><subject>Polymerase chain reaction</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>sequence alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sugars</subject><subject>tissue distribution</subject><subject>transfer DNA</subject><subject>transporters</subject><subject>Yeasts</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUFv3CAQhVHVqNmmPfZWtVx69GYwBuxDD6uoaStFSqQkZwsw7BJ5AYE3avoD-ruL5VXSnpjhffMG8RD6QGBNCDTnMa4J0DXUnIvmFVoRRuuqZk37Gq0ASg1t252itzk_AAChpHmDTkndCWhatkJ_Lg9eTy54OWK9k0nqyST3W85XWPoBm18xmZyXVo5P2WQcLJ52Bm_Hgw7ZVDka7azTeDPd3t10eB98yFLPdm4weErS5xhSMcbO4xjG0fjZY5OkckOI2eV36MTKMZv3x_MM3V9-u7v4UV1df_95sbmqdNPRqWJCc0s7yQbFGQMxaCOUlYKrgQ5GlZYTYoUBo4BwQ6yiohMNt0A7oyyjZ-jr4hsPam8K78vrxj4mt5fpqQ_S9f8r3u36bXjsScdZx8t8tczrFHJOxj6PEujnPPoYS0n7JY_Cf_p33wt9DKAAX46AzFqOtvyVdvmFY6Rmgs7cx4V7yFNIz3pTt4TWbZE_L7KVoZfbVCzub-sSNxBoWcOA_gWD6quW</recordid><startdate>20030901</startdate><enddate>20030901</enddate><creator>Schneidereit, A</creator><creator>Scholz-Starke, J</creator><creator>Buttner, M</creator><general>American Society of Plant Biologists</general><general>American Society of Plant Physiologists</general><general>The American Society for Plant Biologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20030901</creationdate><title>Functional characterization and expression analyses of the glucose-specific AtSTP9 monosaccharide transporter in pollen of Arabidopsis</title><author>Schneidereit, A ; Scholz-Starke, J ; Buttner, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-57c6f39a5db65507dce7bfa76bd3debdce611f7e0eb016e1fb379746f039ebf53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - growth & development</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>AtSTP9 gene</topic><topic>Biological and medical sciences</topic><topic>Cell Biology and Signal Transduction</topic><topic>Cell physiology</topic><topic>Cloning, Molecular</topic><topic>Complementary DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Complementary - genetics</topic><topic>Flowers</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gametophytes</topic><topic>gene expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes, Reporter - genetics</topic><topic>Genes, Reporter - physiology</topic><topic>Genomics</topic><topic>glucose</topic><topic>Glucose - metabolism</topic><topic>insertional mutagenesis</topic><topic>messenger RNA</topic><topic>molecular cloning</topic><topic>Molecular Sequence Data</topic><topic>Monosaccharide Transport Proteins - genetics</topic><topic>Monosaccharide Transport Proteins - metabolism</topic><topic>Monosaccharides</topic><topic>Mutagenesis, Insertional</topic><topic>Mutation</topic><topic>nucleotide sequences</topic><topic>Plant physiology and development</topic><topic>plant proteins</topic><topic>Plants</topic><topic>Plasma membrane and permeation</topic><topic>Pollen</topic><topic>Pollen - genetics</topic><topic>Pollen - growth & development</topic><topic>Pollen - metabolism</topic><topic>pollen germination</topic><topic>Pollen tubes</topic><topic>Polymerase chain reaction</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>sequence alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sugars</topic><topic>tissue distribution</topic><topic>transfer DNA</topic><topic>transporters</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schneidereit, A</creatorcontrib><creatorcontrib>Scholz-Starke, J</creatorcontrib><creatorcontrib>Buttner, M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schneidereit, A</au><au>Scholz-Starke, J</au><au>Buttner, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional characterization and expression analyses of the glucose-specific AtSTP9 monosaccharide transporter in pollen of Arabidopsis</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2003-09-01</date><risdate>2003</risdate><volume>133</volume><issue>1</issue><spage>182</spage><epage>190</epage><pages>182-190</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>A genomic clone and the corresponding cDNA of a new Arabidopsis monosaccharide transporter AtSTP9 were isolated. Transport analysis of the expressed protein in yeast showed that AtSTP9 is an energy-dependent, uncoupler-sensitive, high-affinity monosaccharide transporter with a Km for glucose in the micromolar range. In contrast to all previously characterized monosaccharide transporters, AtSTP9 shows an unusual specificity for glucose. Reverse transcriptase-polymerase chain reaction analyses revealed that AtSTP9 is exclusively expressed in flowers, and a more detailed approach using AtSTP9 promoter/reporter plants clearly showed that AtSTP9 expression is restricted to the male gametophyte. AtSTP9 expression is not found in other floral organs or vegetative tissues. Further localization on the cellular level using a specific antibody revealed that in contrast to the early accumulation of AtSTP9 transcripts in young pollen, the AtSTP9 protein is only found weakly in mature pollen but is most prominent in germinating pollen tubes. This preloading of pollen with mRNAs has been described for genes that are essential for pollen germination and/or pollen tube growth. The pollen-specific expression found for AtSTP9 is also observed for other sugar transporters and indicates that pollen development and germination require a highly regulated supply of sugars.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Biologists</pub><pmid>12970485</pmid><doi>10.1104/pp.103.026674</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Amino Acid Sequence amino acid sequences Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana AtSTP9 gene Biological and medical sciences Cell Biology and Signal Transduction Cell physiology Cloning, Molecular Complementary DNA DNA, Bacterial - genetics DNA, Complementary - chemistry DNA, Complementary - genetics Flowers Fundamental and applied biological sciences. Psychology gametophytes gene expression Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Reporter - genetics Genes, Reporter - physiology Genomics glucose Glucose - metabolism insertional mutagenesis messenger RNA molecular cloning Molecular Sequence Data Monosaccharide Transport Proteins - genetics Monosaccharide Transport Proteins - metabolism Monosaccharides Mutagenesis, Insertional Mutation nucleotide sequences Plant physiology and development plant proteins Plants Plasma membrane and permeation Pollen Pollen - genetics Pollen - growth & development Pollen - metabolism pollen germination Pollen tubes Polymerase chain reaction promoter regions Promoter Regions, Genetic - genetics Reverse Transcriptase Polymerase Chain Reaction sequence alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Sugars tissue distribution transfer DNA transporters Yeasts |
| title | Functional characterization and expression analyses of the glucose-specific AtSTP9 monosaccharide transporter in pollen of Arabidopsis |
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