Determination of Ototoxicity of Common Otic Drops Using Isolated Cochlear Outer Hair Cells
Objectives Otic drops are commonly used not only for otitis externa, but also for otorrhea in the presence of tympanostomy tubes or tympanic membrane perforations. Many studies have demonstrated the ototoxicity of common otic preparations such as Cortisporin® otic drops (Monarch Pharmaceuticals, Bri...
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| Veröffentlicht in: | The Laryngoscope 2001-12, Vol.111 (12), p.2105-2108 |
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| creator | Jinn, Tae Hoon Kim, Paul D. Russell, Paul T. Church, Christopher A. John, Earnest O. Jung, Timothy T. K. |
| description | Objectives Otic drops are commonly used not only for otitis externa, but also for otorrhea in the presence of tympanostomy tubes or tympanic membrane perforations. Many studies have demonstrated the ototoxicity of common otic preparations such as Cortisporin® otic drops (Monarch Pharmaceuticals, Bristol, TN). The purpose of this study was to assess the relative ototoxicity of common otic preparations by direct exposure to isolated cochlear outer hair cells (OHCs).
Methods OHCs from adult chinchilla cochlea were exposed to standard bathing solution (control), acetic acid, Acetasol HC® (Alpharma USPD Inc., Baltimore, MD), Gentacidin® (CIBA Vision Ophthalmics, Atlanta, GA), and Tobradex® (Alcon, Fort Worth, TX). The cells were observed using an inverted microscope, and the images were recorded in digital still‐frame and video, and analyzed on the Image Pro‐Plus 3.0 program (Media Cybernetics, Silver Spring, MD).
Results and Conclusions As measured by time to cell death and change in morphology of OHCs, acetic acid with or without hydrocortisone was most toxic to OHCs. Cortisporin was more cytotoxic than gentamicin and Tobradex. |
| doi_str_mv | 10.1097/00005537-200112000-00004 |
| format | Article |
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Methods OHCs from adult chinchilla cochlea were exposed to standard bathing solution (control), acetic acid, Acetasol HC® (Alpharma USPD Inc., Baltimore, MD), Gentacidin® (CIBA Vision Ophthalmics, Atlanta, GA), and Tobradex® (Alcon, Fort Worth, TX). The cells were observed using an inverted microscope, and the images were recorded in digital still‐frame and video, and analyzed on the Image Pro‐Plus 3.0 program (Media Cybernetics, Silver Spring, MD).
Results and Conclusions As measured by time to cell death and change in morphology of OHCs, acetic acid with or without hydrocortisone was most toxic to OHCs. Cortisporin was more cytotoxic than gentamicin and Tobradex.</description><identifier>ISSN: 0023-852X</identifier><identifier>EISSN: 1531-4995</identifier><identifier>DOI: 10.1097/00005537-200112000-00004</identifier><identifier>PMID: 11802005</identifier><identifier>CODEN: LARYA8</identifier><language>eng</language><publisher>Hoboken, NJ: John Wiley & Sons, Inc</publisher><subject>Acetasol HC ; acetic acid ; Acetic Acid - toxicity ; Administration, Topical ; Animals ; Biological and medical sciences ; Cell Size - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; Chinchilla ; Ciloxan ; Cipro HC ; Drug Combinations ; Drug toxicity and drugs side effects treatment ; Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology ; Floxin ; gentamicin ; Gentamicins - toxicity ; Hair Cells, Auditory, Outer - diagnostic imaging ; Hair Cells, Auditory, Outer - drug effects ; Hydrocortisone - toxicity ; isolated outer hair cells ; Medical sciences ; Neomycin - toxicity ; Non tumoral diseases ; otic drops ; Otorhinolaryngology. Stomatology ; Ototoxicity ; Pharmacology. Drug treatments ; Polymyxin B - toxicity ; Tobradex ; Tobramycin - toxicity ; Toxicity: respiratory system, ent, stomatology ; Ultrasonography</subject><ispartof>The Laryngoscope, 2001-12, Vol.111 (12), p.2105-2108</ispartof><rights>Copyright © 2001 The Triological Society</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4404-88c5e85cca3c962c23e0b9c5ab7189ab03cdf85656be93262c3e4f9c361cb6373</citedby><cites>FETCH-LOGICAL-c4404-88c5e85cca3c962c23e0b9c5ab7189ab03cdf85656be93262c3e4f9c361cb6373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1097%2F00005537-200112000-00004$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1097%2F00005537-200112000-00004$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13413409$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11802005$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jinn, Tae Hoon</creatorcontrib><creatorcontrib>Kim, Paul D.</creatorcontrib><creatorcontrib>Russell, Paul T.</creatorcontrib><creatorcontrib>Church, Christopher A.</creatorcontrib><creatorcontrib>John, Earnest O.</creatorcontrib><creatorcontrib>Jung, Timothy T. K.</creatorcontrib><title>Determination of Ototoxicity of Common Otic Drops Using Isolated Cochlear Outer Hair Cells</title><title>The Laryngoscope</title><addtitle>The Laryngoscope</addtitle><description>Objectives Otic drops are commonly used not only for otitis externa, but also for otorrhea in the presence of tympanostomy tubes or tympanic membrane perforations. Many studies have demonstrated the ototoxicity of common otic preparations such as Cortisporin® otic drops (Monarch Pharmaceuticals, Bristol, TN). The purpose of this study was to assess the relative ototoxicity of common otic preparations by direct exposure to isolated cochlear outer hair cells (OHCs).
Methods OHCs from adult chinchilla cochlea were exposed to standard bathing solution (control), acetic acid, Acetasol HC® (Alpharma USPD Inc., Baltimore, MD), Gentacidin® (CIBA Vision Ophthalmics, Atlanta, GA), and Tobradex® (Alcon, Fort Worth, TX). The cells were observed using an inverted microscope, and the images were recorded in digital still‐frame and video, and analyzed on the Image Pro‐Plus 3.0 program (Media Cybernetics, Silver Spring, MD).
Results and Conclusions As measured by time to cell death and change in morphology of OHCs, acetic acid with or without hydrocortisone was most toxic to OHCs. Cortisporin was more cytotoxic than gentamicin and Tobradex.</description><subject>Acetasol HC</subject><subject>acetic acid</subject><subject>Acetic Acid - toxicity</subject><subject>Administration, Topical</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Size - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Chinchilla</subject><subject>Ciloxan</subject><subject>Cipro HC</subject><subject>Drug Combinations</subject><subject>Drug toxicity and drugs side effects treatment</subject><subject>Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology</subject><subject>Floxin</subject><subject>gentamicin</subject><subject>Gentamicins - toxicity</subject><subject>Hair Cells, Auditory, Outer - diagnostic imaging</subject><subject>Hair Cells, Auditory, Outer - drug effects</subject><subject>Hydrocortisone - toxicity</subject><subject>isolated outer hair cells</subject><subject>Medical sciences</subject><subject>Neomycin - toxicity</subject><subject>Non tumoral diseases</subject><subject>otic drops</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Ototoxicity</subject><subject>Pharmacology. Drug treatments</subject><subject>Polymyxin B - toxicity</subject><subject>Tobradex</subject><subject>Tobramycin - toxicity</subject><subject>Toxicity: respiratory system, ent, stomatology</subject><subject>Ultrasonography</subject><issn>0023-852X</issn><issn>1531-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF1PwyAYhYnR6Jz-BcONl9WXAm25NJ26JYtNpsaPG0IZVbRdF6hx-_dSN_VWQiAcnnPy5iCECZwREOk5hMU5TaMYgJBwQNRLbAcNCKckYkLwXTQAiGmU8fjxAB16_xbYlHLYRweEZBBcfICeR6YzrrEL1dl2gdsKF13btSurbbfun3nbNOGj6KzGI9cuPb73dvGCJ76tVWfmAdCvtVEOFx8hCY-VdTg3de2P0F6lam-Ot_cQ3V9d3uXjaFpcT_KLaaQZAxZlmeYm41orqkUS65gaKIXmqkxJJlQJVM-rjCc8KY2gcSCoYZXQNCG6TGhKhyjb5GrXeu9MJZfONsqtJQHZ1yV_6pK_dX1LLFhPNtblR9mY-Z9x208ATreA8lrVlVMLbf0fR1nYIAI32nCftjbrfw8gpxezJ84ZIb3azxNtYqzvzOo3Rrl3maQ05fLh5lrC8-gW4jGTM_oFXJKTnw</recordid><startdate>200112</startdate><enddate>200112</enddate><creator>Jinn, Tae Hoon</creator><creator>Kim, Paul D.</creator><creator>Russell, Paul T.</creator><creator>Church, Christopher A.</creator><creator>John, Earnest O.</creator><creator>Jung, Timothy T. K.</creator><general>John Wiley & Sons, Inc</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>200112</creationdate><title>Determination of Ototoxicity of Common Otic Drops Using Isolated Cochlear Outer Hair Cells</title><author>Jinn, Tae Hoon ; Kim, Paul D. ; Russell, Paul T. ; Church, Christopher A. ; John, Earnest O. ; Jung, Timothy T. K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4404-88c5e85cca3c962c23e0b9c5ab7189ab03cdf85656be93262c3e4f9c361cb6373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Acetasol HC</topic><topic>acetic acid</topic><topic>Acetic Acid - toxicity</topic><topic>Administration, Topical</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Size - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Chinchilla</topic><topic>Ciloxan</topic><topic>Cipro HC</topic><topic>Drug Combinations</topic><topic>Drug toxicity and drugs side effects treatment</topic><topic>Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology</topic><topic>Floxin</topic><topic>gentamicin</topic><topic>Gentamicins - toxicity</topic><topic>Hair Cells, Auditory, Outer - diagnostic imaging</topic><topic>Hair Cells, Auditory, Outer - drug effects</topic><topic>Hydrocortisone - toxicity</topic><topic>isolated outer hair cells</topic><topic>Medical sciences</topic><topic>Neomycin - toxicity</topic><topic>Non tumoral diseases</topic><topic>otic drops</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Ototoxicity</topic><topic>Pharmacology. Drug treatments</topic><topic>Polymyxin B - toxicity</topic><topic>Tobradex</topic><topic>Tobramycin - toxicity</topic><topic>Toxicity: respiratory system, ent, stomatology</topic><topic>Ultrasonography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jinn, Tae Hoon</creatorcontrib><creatorcontrib>Kim, Paul D.</creatorcontrib><creatorcontrib>Russell, Paul T.</creatorcontrib><creatorcontrib>Church, Christopher A.</creatorcontrib><creatorcontrib>John, Earnest O.</creatorcontrib><creatorcontrib>Jung, Timothy T. K.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>The Laryngoscope</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jinn, Tae Hoon</au><au>Kim, Paul D.</au><au>Russell, Paul T.</au><au>Church, Christopher A.</au><au>John, Earnest O.</au><au>Jung, Timothy T. K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Ototoxicity of Common Otic Drops Using Isolated Cochlear Outer Hair Cells</atitle><jtitle>The Laryngoscope</jtitle><addtitle>The Laryngoscope</addtitle><date>2001-12</date><risdate>2001</risdate><volume>111</volume><issue>12</issue><spage>2105</spage><epage>2108</epage><pages>2105-2108</pages><issn>0023-852X</issn><eissn>1531-4995</eissn><coden>LARYA8</coden><abstract>Objectives Otic drops are commonly used not only for otitis externa, but also for otorrhea in the presence of tympanostomy tubes or tympanic membrane perforations. Many studies have demonstrated the ototoxicity of common otic preparations such as Cortisporin® otic drops (Monarch Pharmaceuticals, Bristol, TN). The purpose of this study was to assess the relative ototoxicity of common otic preparations by direct exposure to isolated cochlear outer hair cells (OHCs).
Methods OHCs from adult chinchilla cochlea were exposed to standard bathing solution (control), acetic acid, Acetasol HC® (Alpharma USPD Inc., Baltimore, MD), Gentacidin® (CIBA Vision Ophthalmics, Atlanta, GA), and Tobradex® (Alcon, Fort Worth, TX). The cells were observed using an inverted microscope, and the images were recorded in digital still‐frame and video, and analyzed on the Image Pro‐Plus 3.0 program (Media Cybernetics, Silver Spring, MD).
Results and Conclusions As measured by time to cell death and change in morphology of OHCs, acetic acid with or without hydrocortisone was most toxic to OHCs. Cortisporin was more cytotoxic than gentamicin and Tobradex.</abstract><cop>Hoboken, NJ</cop><pub>John Wiley & Sons, Inc</pub><pmid>11802005</pmid><doi>10.1097/00005537-200112000-00004</doi><tpages>4</tpages></addata></record> |
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| ispartof | The Laryngoscope, 2001-12, Vol.111 (12), p.2105-2108 |
| issn | 0023-852X 1531-4995 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Journals@Ovid Complete |
| subjects | Acetasol HC acetic acid Acetic Acid - toxicity Administration, Topical Animals Biological and medical sciences Cell Size - drug effects Cell Survival - drug effects Cells, Cultured Chinchilla Ciloxan Cipro HC Drug Combinations Drug toxicity and drugs side effects treatment Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology Floxin gentamicin Gentamicins - toxicity Hair Cells, Auditory, Outer - diagnostic imaging Hair Cells, Auditory, Outer - drug effects Hydrocortisone - toxicity isolated outer hair cells Medical sciences Neomycin - toxicity Non tumoral diseases otic drops Otorhinolaryngology. Stomatology Ototoxicity Pharmacology. Drug treatments Polymyxin B - toxicity Tobradex Tobramycin - toxicity Toxicity: respiratory system, ent, stomatology Ultrasonography |
| title | Determination of Ototoxicity of Common Otic Drops Using Isolated Cochlear Outer Hair Cells |
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