Experimental Investigation of Cerebral Contusion: Histopathological and Immunohistochemical Evaluation of Dynamic Cortical Deformation
We used a new approach, termed dynamic cortical deformation (DCD), to study the neuronal, vascular, and glial responses that occur in focal cerebral contusions. DCD produces experimental contusion by rapidly deforming the cerebral cortex with a transient, nonablative vacuum pulse of short duration (...
Gespeichert in:
| Veröffentlicht in: | Journal of neuropathology and experimental neurology 1999-02, Vol.58 (2), p.153-164 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 164 |
|---|---|
| container_issue | 2 |
| container_start_page | 153 |
| container_title | Journal of neuropathology and experimental neurology |
| container_volume | 58 |
| creator | Shreiber, David I Bain, Allison C Ross, Douglas T Smith, Douglas H Gennarelli, T A McIntosh, Tracy K Meaney, David F |
| description | We used a new approach, termed dynamic cortical deformation (DCD), to study the neuronal, vascular, and glial responses that occur in focal cerebral contusions. DCD produces experimental contusion by rapidly deforming the cerebral cortex with a transient, nonablative vacuum pulse of short duration (25 milliseconds) to mimic the circumstances of traumatic injury. A neuropathological evaluation was performed on brain tissue from adult rats sacrificed 3 days following induction of either moderate (4 psi, n=6) or high (8 psi, n=6) severity DCD. In all animals, DCD produced focal hemorrhagic lesions at the vacuum site without overt damage to other regions. Examination of histological sections showed localized gross tissue and neuronal loss in the cortex at the injury site, with the volume of cell loss dependent upon the mechanical loading (p |
| doi_str_mv | 10.1097/00005072-199902000-00005 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1097_00005072_199902000_00005</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10029098</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4355-730853d9f8cbff1d9db3bf80381f8f3236f66fb00b11094a31332cd169dd427c3</originalsourceid><addsrcrecordid>eNp1UU1PwyAYJkbj5vQvmB68VqH0A7yZbrolS7zouaEU1mpbGqCb-wP-blk7Py5ygff5St4HADwEbxGkyR10J4JJ4CNKKQzc5A_QCZiiKAr9OErIKZhCGAQ-hjGdgAtj3pyCQhqegwlyjHuSKfhcfHRCV41oLau9VbsVxlYbZivVekp6qdAi145JVWt749B7b1kZqzpmS1WrTcUdydrCWzVN36rywPFSNAO-2LK6_8ma71vmcBel7UDPhVS6GfhLcCZZbcTV8Z6B18fFS7r0189Pq_Rh7fMQR5GfYEgiXFBJeC4lKmiR41wSiAmSROIAxzKOZQ5hjlxPIcMI44AXKKZFEQYJxzNAxlyulTFayKxzyzO9zxDMDtVm39VmP9WOkLNej9auzxtR_DGOXTrBzVHAjFtPatbyyvzqYhITTJ0sHGU7VVuhzXvd74TOSsFqW2b__Sz-AhdFk5I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Experimental Investigation of Cerebral Contusion: Histopathological and Immunohistochemical Evaluation of Dynamic Cortical Deformation</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Journals@Ovid Complete</source><creator>Shreiber, David I ; Bain, Allison C ; Ross, Douglas T ; Smith, Douglas H ; Gennarelli, T A ; McIntosh, Tracy K ; Meaney, David F</creator><creatorcontrib>Shreiber, David I ; Bain, Allison C ; Ross, Douglas T ; Smith, Douglas H ; Gennarelli, T A ; McIntosh, Tracy K ; Meaney, David F</creatorcontrib><description>We used a new approach, termed dynamic cortical deformation (DCD), to study the neuronal, vascular, and glial responses that occur in focal cerebral contusions. DCD produces experimental contusion by rapidly deforming the cerebral cortex with a transient, nonablative vacuum pulse of short duration (25 milliseconds) to mimic the circumstances of traumatic injury. A neuropathological evaluation was performed on brain tissue from adult rats sacrificed 3 days following induction of either moderate (4 psi, n=6) or high (8 psi, n=6) severity DCD. In all animals, DCD produced focal hemorrhagic lesions at the vacuum site without overt damage to other regions. Examination of histological sections showed localized gross tissue and neuronal loss in the cortex at the injury site, with the volume of cell loss dependent upon the mechanical loading (p<0.001). Axonal pathology shown with neurofilament immunostaining (SMI-31 and SMI- 32) was observed in the subcortical white matter inferior to the injury site and in the ipsilateral internal capsule. No axonal injury was observed in the contralateral hemisphere or in any remote regions. Glial fibrillary acidic protein (GFAP) immunostaining revealed widespread reactive astrocytosis surrounding the necrotic region in the ipsilateral cortex. This analysis confirms that rapid mechanical deformation of the cortex induces focal contusions in the absence of primary damage to remote areas 3 days following injury. Although it is suggested that massive release of neurotoxic substances from a contusion may cause damage throughout the brain, these data emphasize the importance of combined injury mechanisms, e.g. mechanical distortion and excitatory amino acid mediated damage, that underlie the complex pathology patterns observed in traumatic brain injury.</description><identifier>ISSN: 0022-3069</identifier><identifier>EISSN: 1554-6578</identifier><identifier>DOI: 10.1097/00005072-199902000-00005</identifier><identifier>PMID: 10029098</identifier><identifier>CODEN: JNENAD</identifier><language>eng</language><publisher>Hagerstown, MD: American Association of Neuropathologists, Inc</publisher><subject>Animals ; Astrocytes - chemistry ; Biological and medical sciences ; Biomechanical Phenomena ; Brain Chemistry - physiology ; Brain Concussion - pathology ; Brain Concussion - physiopathology ; Cerebral Cortex - cytology ; Cerebral Cortex - pathology ; Cerebral Hemorrhage - pathology ; Cerebral Hemorrhage - physiopathology ; Disease Models, Animal ; Glial Fibrillary Acidic Protein - analysis ; Injuries of the nervous system and the skull. Diseases due to physical agents ; Male ; Medical sciences ; Necrosis ; Neurofilament Proteins - analysis ; Neurons - chemistry ; Rats ; Rats, Sprague-Dawley ; Traumas. Diseases due to physical agents</subject><ispartof>Journal of neuropathology and experimental neurology, 1999-02, Vol.58 (2), p.153-164</ispartof><rights>1999 American Association of Neuropathologists, Inc</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4355-730853d9f8cbff1d9db3bf80381f8f3236f66fb00b11094a31332cd169dd427c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1686839$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10029098$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shreiber, David I</creatorcontrib><creatorcontrib>Bain, Allison C</creatorcontrib><creatorcontrib>Ross, Douglas T</creatorcontrib><creatorcontrib>Smith, Douglas H</creatorcontrib><creatorcontrib>Gennarelli, T A</creatorcontrib><creatorcontrib>McIntosh, Tracy K</creatorcontrib><creatorcontrib>Meaney, David F</creatorcontrib><title>Experimental Investigation of Cerebral Contusion: Histopathological and Immunohistochemical Evaluation of Dynamic Cortical Deformation</title><title>Journal of neuropathology and experimental neurology</title><addtitle>J Neuropathol Exp Neurol</addtitle><description>We used a new approach, termed dynamic cortical deformation (DCD), to study the neuronal, vascular, and glial responses that occur in focal cerebral contusions. DCD produces experimental contusion by rapidly deforming the cerebral cortex with a transient, nonablative vacuum pulse of short duration (25 milliseconds) to mimic the circumstances of traumatic injury. A neuropathological evaluation was performed on brain tissue from adult rats sacrificed 3 days following induction of either moderate (4 psi, n=6) or high (8 psi, n=6) severity DCD. In all animals, DCD produced focal hemorrhagic lesions at the vacuum site without overt damage to other regions. Examination of histological sections showed localized gross tissue and neuronal loss in the cortex at the injury site, with the volume of cell loss dependent upon the mechanical loading (p<0.001). Axonal pathology shown with neurofilament immunostaining (SMI-31 and SMI- 32) was observed in the subcortical white matter inferior to the injury site and in the ipsilateral internal capsule. No axonal injury was observed in the contralateral hemisphere or in any remote regions. Glial fibrillary acidic protein (GFAP) immunostaining revealed widespread reactive astrocytosis surrounding the necrotic region in the ipsilateral cortex. This analysis confirms that rapid mechanical deformation of the cortex induces focal contusions in the absence of primary damage to remote areas 3 days following injury. Although it is suggested that massive release of neurotoxic substances from a contusion may cause damage throughout the brain, these data emphasize the importance of combined injury mechanisms, e.g. mechanical distortion and excitatory amino acid mediated damage, that underlie the complex pathology patterns observed in traumatic brain injury.</description><subject>Animals</subject><subject>Astrocytes - chemistry</subject><subject>Biological and medical sciences</subject><subject>Biomechanical Phenomena</subject><subject>Brain Chemistry - physiology</subject><subject>Brain Concussion - pathology</subject><subject>Brain Concussion - physiopathology</subject><subject>Cerebral Cortex - cytology</subject><subject>Cerebral Cortex - pathology</subject><subject>Cerebral Hemorrhage - pathology</subject><subject>Cerebral Hemorrhage - physiopathology</subject><subject>Disease Models, Animal</subject><subject>Glial Fibrillary Acidic Protein - analysis</subject><subject>Injuries of the nervous system and the skull. Diseases due to physical agents</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Necrosis</subject><subject>Neurofilament Proteins - analysis</subject><subject>Neurons - chemistry</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Traumas. Diseases due to physical agents</subject><issn>0022-3069</issn><issn>1554-6578</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UU1PwyAYJkbj5vQvmB68VqH0A7yZbrolS7zouaEU1mpbGqCb-wP-blk7Py5ygff5St4HADwEbxGkyR10J4JJ4CNKKQzc5A_QCZiiKAr9OErIKZhCGAQ-hjGdgAtj3pyCQhqegwlyjHuSKfhcfHRCV41oLau9VbsVxlYbZivVekp6qdAi145JVWt749B7b1kZqzpmS1WrTcUdydrCWzVN36rywPFSNAO-2LK6_8ma71vmcBel7UDPhVS6GfhLcCZZbcTV8Z6B18fFS7r0189Pq_Rh7fMQR5GfYEgiXFBJeC4lKmiR41wSiAmSROIAxzKOZQ5hjlxPIcMI44AXKKZFEQYJxzNAxlyulTFayKxzyzO9zxDMDtVm39VmP9WOkLNej9auzxtR_DGOXTrBzVHAjFtPatbyyvzqYhITTJ0sHGU7VVuhzXvd74TOSsFqW2b__Sz-AhdFk5I</recordid><startdate>199902</startdate><enddate>199902</enddate><creator>Shreiber, David I</creator><creator>Bain, Allison C</creator><creator>Ross, Douglas T</creator><creator>Smith, Douglas H</creator><creator>Gennarelli, T A</creator><creator>McIntosh, Tracy K</creator><creator>Meaney, David F</creator><general>American Association of Neuropathologists, Inc</general><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>199902</creationdate><title>Experimental Investigation of Cerebral Contusion: Histopathological and Immunohistochemical Evaluation of Dynamic Cortical Deformation</title><author>Shreiber, David I ; Bain, Allison C ; Ross, Douglas T ; Smith, Douglas H ; Gennarelli, T A ; McIntosh, Tracy K ; Meaney, David F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4355-730853d9f8cbff1d9db3bf80381f8f3236f66fb00b11094a31332cd169dd427c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Astrocytes - chemistry</topic><topic>Biological and medical sciences</topic><topic>Biomechanical Phenomena</topic><topic>Brain Chemistry - physiology</topic><topic>Brain Concussion - pathology</topic><topic>Brain Concussion - physiopathology</topic><topic>Cerebral Cortex - cytology</topic><topic>Cerebral Cortex - pathology</topic><topic>Cerebral Hemorrhage - pathology</topic><topic>Cerebral Hemorrhage - physiopathology</topic><topic>Disease Models, Animal</topic><topic>Glial Fibrillary Acidic Protein - analysis</topic><topic>Injuries of the nervous system and the skull. Diseases due to physical agents</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Necrosis</topic><topic>Neurofilament Proteins - analysis</topic><topic>Neurons - chemistry</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Traumas. Diseases due to physical agents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shreiber, David I</creatorcontrib><creatorcontrib>Bain, Allison C</creatorcontrib><creatorcontrib>Ross, Douglas T</creatorcontrib><creatorcontrib>Smith, Douglas H</creatorcontrib><creatorcontrib>Gennarelli, T A</creatorcontrib><creatorcontrib>McIntosh, Tracy K</creatorcontrib><creatorcontrib>Meaney, David F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of neuropathology and experimental neurology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shreiber, David I</au><au>Bain, Allison C</au><au>Ross, Douglas T</au><au>Smith, Douglas H</au><au>Gennarelli, T A</au><au>McIntosh, Tracy K</au><au>Meaney, David F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Experimental Investigation of Cerebral Contusion: Histopathological and Immunohistochemical Evaluation of Dynamic Cortical Deformation</atitle><jtitle>Journal of neuropathology and experimental neurology</jtitle><addtitle>J Neuropathol Exp Neurol</addtitle><date>1999-02</date><risdate>1999</risdate><volume>58</volume><issue>2</issue><spage>153</spage><epage>164</epage><pages>153-164</pages><issn>0022-3069</issn><eissn>1554-6578</eissn><coden>JNENAD</coden><abstract>We used a new approach, termed dynamic cortical deformation (DCD), to study the neuronal, vascular, and glial responses that occur in focal cerebral contusions. DCD produces experimental contusion by rapidly deforming the cerebral cortex with a transient, nonablative vacuum pulse of short duration (25 milliseconds) to mimic the circumstances of traumatic injury. A neuropathological evaluation was performed on brain tissue from adult rats sacrificed 3 days following induction of either moderate (4 psi, n=6) or high (8 psi, n=6) severity DCD. In all animals, DCD produced focal hemorrhagic lesions at the vacuum site without overt damage to other regions. Examination of histological sections showed localized gross tissue and neuronal loss in the cortex at the injury site, with the volume of cell loss dependent upon the mechanical loading (p<0.001). Axonal pathology shown with neurofilament immunostaining (SMI-31 and SMI- 32) was observed in the subcortical white matter inferior to the injury site and in the ipsilateral internal capsule. No axonal injury was observed in the contralateral hemisphere or in any remote regions. Glial fibrillary acidic protein (GFAP) immunostaining revealed widespread reactive astrocytosis surrounding the necrotic region in the ipsilateral cortex. This analysis confirms that rapid mechanical deformation of the cortex induces focal contusions in the absence of primary damage to remote areas 3 days following injury. Although it is suggested that massive release of neurotoxic substances from a contusion may cause damage throughout the brain, these data emphasize the importance of combined injury mechanisms, e.g. mechanical distortion and excitatory amino acid mediated damage, that underlie the complex pathology patterns observed in traumatic brain injury.</abstract><cop>Hagerstown, MD</cop><pub>American Association of Neuropathologists, Inc</pub><pmid>10029098</pmid><doi>10.1097/00005072-199902000-00005</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3069 |
| ispartof | Journal of neuropathology and experimental neurology, 1999-02, Vol.58 (2), p.153-164 |
| issn | 0022-3069 1554-6578 |
| language | eng |
| recordid | cdi_crossref_primary_10_1097_00005072_199902000_00005 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Journals@Ovid Complete |
| subjects | Animals Astrocytes - chemistry Biological and medical sciences Biomechanical Phenomena Brain Chemistry - physiology Brain Concussion - pathology Brain Concussion - physiopathology Cerebral Cortex - cytology Cerebral Cortex - pathology Cerebral Hemorrhage - pathology Cerebral Hemorrhage - physiopathology Disease Models, Animal Glial Fibrillary Acidic Protein - analysis Injuries of the nervous system and the skull. Diseases due to physical agents Male Medical sciences Necrosis Neurofilament Proteins - analysis Neurons - chemistry Rats Rats, Sprague-Dawley Traumas. Diseases due to physical agents |
| title | Experimental Investigation of Cerebral Contusion: Histopathological and Immunohistochemical Evaluation of Dynamic Cortical Deformation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T00%3A50%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Experimental%20Investigation%20of%20Cerebral%20Contusion:%20Histopathological%20and%20Immunohistochemical%20Evaluation%20of%20Dynamic%20Cortical%20Deformation&rft.jtitle=Journal%20of%20neuropathology%20and%20experimental%20neurology&rft.au=Shreiber,%20David%20I&rft.date=1999-02&rft.volume=58&rft.issue=2&rft.spage=153&rft.epage=164&rft.pages=153-164&rft.issn=0022-3069&rft.eissn=1554-6578&rft.coden=JNENAD&rft_id=info:doi/10.1097/00005072-199902000-00005&rft_dat=%3Cpubmed_cross%3E10029098%3C/pubmed_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/10029098&rfr_iscdi=true |