Suppression of Human Corneal Epithelial Proliferation with Breast Carcinoma Immunotoxin
We examined the effects of the immunotoxin 260F9 Mabrecombinant ricin A (developed against human breast carcinoma) on proliferating and confluent human corneal epithelium (HCE) cells in vitro. HCE cells derived from explants of discarded human donor corneoscleral rims were established as proliferati...
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| Veröffentlicht in: | Cornea 1993-09, Vol.12 (5), p.391-396 |
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| container_title | Cornea |
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| creator | Fulcher, Samuel Foulks, Gary N Wilkerson, Mark Cobo, L Michael Houston, L L Hatchell, Diane |
| description | We examined the effects of the immunotoxin 260F9 Mabrecombinant ricin A (developed against human breast carcinoma) on proliferating and confluent human corneal epithelium (HCE) cells in vitro. HCE cells derived from explants of discarded human donor corneoscleral rims were established as proliferating and confluent cell cultures, and were exposed continuously for 7 days to immunotoxin. Final cell counts at day 7, and thymidine uptake measured at days 1 and 7 postexposure, showed >95% suppression of proliferating cells at an immunotoxin concentration of 10 ng/ml, with confluent HCE cells relatively unaffected. This immunotoxin may prove useful in treatment of proliferative ocular epithelial diseases such as epithelial downgrowth or squamous cell carcinoma of the ocular surface. |
| doi_str_mv | 10.1097/00003226-199309000-00004 |
| format | Article |
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HCE cells derived from explants of discarded human donor corneoscleral rims were established as proliferating and confluent cell cultures, and were exposed continuously for 7 days to immunotoxin. Final cell counts at day 7, and thymidine uptake measured at days 1 and 7 postexposure, showed >95% suppression of proliferating cells at an immunotoxin concentration of 10 ng/ml, with confluent HCE cells relatively unaffected. This immunotoxin may prove useful in treatment of proliferative ocular epithelial diseases such as epithelial downgrowth or squamous cell carcinoma of the ocular surface.</description><identifier>ISSN: 0277-3740</identifier><identifier>EISSN: 1536-4798</identifier><identifier>DOI: 10.1097/00003226-199309000-00004</identifier><identifier>PMID: 8306659</identifier><language>eng</language><publisher>United States: Lippincott-Raven Publishers</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Antigens, Neoplasm - immunology ; Breast Neoplasms - immunology ; Carcinoma - immunology ; Cell Count ; Cell Division - drug effects ; Cells, Cultured ; Cornea - cytology ; DNA - biosynthesis ; DNA Replication - drug effects ; Female ; Humans ; Immunotoxins - pharmacology ; Mice ; Mice, Inbred BALB C ; Recombinant Proteins - pharmacology ; Ricin - pharmacology</subject><ispartof>Cornea, 1993-09, Vol.12 (5), p.391-396</ispartof><rights>Lippincott-Raven Publishers.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3554-2b2d2d48425d48e2b7c57f4a16849cf6c64595bfdfa3712769536a737118e7ba3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8306659$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fulcher, Samuel</creatorcontrib><creatorcontrib>Foulks, Gary N</creatorcontrib><creatorcontrib>Wilkerson, Mark</creatorcontrib><creatorcontrib>Cobo, L Michael</creatorcontrib><creatorcontrib>Houston, L L</creatorcontrib><creatorcontrib>Hatchell, Diane</creatorcontrib><title>Suppression of Human Corneal Epithelial Proliferation with Breast Carcinoma Immunotoxin</title><title>Cornea</title><addtitle>Cornea</addtitle><description>We examined the effects of the immunotoxin 260F9 Mabrecombinant ricin A (developed against human breast carcinoma) on proliferating and confluent human corneal epithelium (HCE) cells in vitro. HCE cells derived from explants of discarded human donor corneoscleral rims were established as proliferating and confluent cell cultures, and were exposed continuously for 7 days to immunotoxin. Final cell counts at day 7, and thymidine uptake measured at days 1 and 7 postexposure, showed >95% suppression of proliferating cells at an immunotoxin concentration of 10 ng/ml, with confluent HCE cells relatively unaffected. This immunotoxin may prove useful in treatment of proliferative ocular epithelial diseases such as epithelial downgrowth or squamous cell carcinoma of the ocular surface.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens, Neoplasm - immunology</subject><subject>Breast Neoplasms - immunology</subject><subject>Carcinoma - immunology</subject><subject>Cell Count</subject><subject>Cell Division - drug effects</subject><subject>Cells, Cultured</subject><subject>Cornea - cytology</subject><subject>DNA - biosynthesis</subject><subject>DNA Replication - drug effects</subject><subject>Female</subject><subject>Humans</subject><subject>Immunotoxins - pharmacology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Ricin - pharmacology</subject><issn>0277-3740</issn><issn>1536-4798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kd9KwzAUxoMoc04fQcgLVPM_zaWW6QYDBRUvS9omrNo2JWmpvr2Zm7szF8k53znfgfMLABCjG4yUvEXxUEJEgpWiSMUs2UnsBMwxpyJhUqWnYI6IlAmVDJ2DixA-YoeUgszALKVICK7m4P1l7HtvQqhdB52Fq7HVHcyc74xu4LKvh61p6hg-e9fU1ng97DqnqMN7b3QYYKZ9WXeu1XDdtmPnBvdVd5fgzOommKvDuwBvD8vXbJVsnh7X2d0mKSnnLCEFqUjFUkZ4vA0pZMmlZRqLlKnSilIwrnhhK6upxEQKFbfTMsY4NbLQdAHS_dzSuxC8sXnv61b77xyjfIcq_0OVH1H9Sixar_fWfixaUx2NBzaxzvb1yTWD8eGzGSfj820EM2zz_36A_gC_WnQv</recordid><startdate>199309</startdate><enddate>199309</enddate><creator>Fulcher, Samuel</creator><creator>Foulks, Gary N</creator><creator>Wilkerson, Mark</creator><creator>Cobo, L Michael</creator><creator>Houston, L L</creator><creator>Hatchell, Diane</creator><general>Lippincott-Raven Publishers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>199309</creationdate><title>Suppression of Human Corneal Epithelial Proliferation with Breast Carcinoma Immunotoxin</title><author>Fulcher, Samuel ; Foulks, Gary N ; Wilkerson, Mark ; Cobo, L Michael ; Houston, L L ; Hatchell, Diane</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3554-2b2d2d48425d48e2b7c57f4a16849cf6c64595bfdfa3712769536a737118e7ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens, Neoplasm - immunology</topic><topic>Breast Neoplasms - immunology</topic><topic>Carcinoma - immunology</topic><topic>Cell Count</topic><topic>Cell Division - drug effects</topic><topic>Cells, Cultured</topic><topic>Cornea - cytology</topic><topic>DNA - biosynthesis</topic><topic>DNA Replication - drug effects</topic><topic>Female</topic><topic>Humans</topic><topic>Immunotoxins - pharmacology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Ricin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fulcher, Samuel</creatorcontrib><creatorcontrib>Foulks, Gary N</creatorcontrib><creatorcontrib>Wilkerson, Mark</creatorcontrib><creatorcontrib>Cobo, L Michael</creatorcontrib><creatorcontrib>Houston, L L</creatorcontrib><creatorcontrib>Hatchell, Diane</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Cornea</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fulcher, Samuel</au><au>Foulks, Gary N</au><au>Wilkerson, Mark</au><au>Cobo, L Michael</au><au>Houston, L L</au><au>Hatchell, Diane</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Suppression of Human Corneal Epithelial Proliferation with Breast Carcinoma Immunotoxin</atitle><jtitle>Cornea</jtitle><addtitle>Cornea</addtitle><date>1993-09</date><risdate>1993</risdate><volume>12</volume><issue>5</issue><spage>391</spage><epage>396</epage><pages>391-396</pages><issn>0277-3740</issn><eissn>1536-4798</eissn><abstract>We examined the effects of the immunotoxin 260F9 Mabrecombinant ricin A (developed against human breast carcinoma) on proliferating and confluent human corneal epithelium (HCE) cells in vitro. HCE cells derived from explants of discarded human donor corneoscleral rims were established as proliferating and confluent cell cultures, and were exposed continuously for 7 days to immunotoxin. Final cell counts at day 7, and thymidine uptake measured at days 1 and 7 postexposure, showed >95% suppression of proliferating cells at an immunotoxin concentration of 10 ng/ml, with confluent HCE cells relatively unaffected. This immunotoxin may prove useful in treatment of proliferative ocular epithelial diseases such as epithelial downgrowth or squamous cell carcinoma of the ocular surface.</abstract><cop>United States</cop><pub>Lippincott-Raven Publishers</pub><pmid>8306659</pmid><doi>10.1097/00003226-199309000-00004</doi><tpages>6</tpages></addata></record> |
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| ispartof | Cornea, 1993-09, Vol.12 (5), p.391-396 |
| issn | 0277-3740 1536-4798 |
| language | eng |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Animals Antibodies, Monoclonal - immunology Antigens, Neoplasm - immunology Breast Neoplasms - immunology Carcinoma - immunology Cell Count Cell Division - drug effects Cells, Cultured Cornea - cytology DNA - biosynthesis DNA Replication - drug effects Female Humans Immunotoxins - pharmacology Mice Mice, Inbred BALB C Recombinant Proteins - pharmacology Ricin - pharmacology |
| title | Suppression of Human Corneal Epithelial Proliferation with Breast Carcinoma Immunotoxin |
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