Toxicity and Pharmacokinetics of Subconjunctival Amphotericin B: An Experimental Study

The pharmacokinetics and toxicity of subconjunctival (S/C) amphotericin B (AmB) were evaluated in Dutch-belted rabbits. Following the S /C injection of 1.500 µg of AmB, corneal and aqueous levels were determined by bioassay. The highest levels were present in the periphery of debrided corneas at 1 h (90.12 ± 2.4 µg/g). The debrided central cornea contained 30.84 µg/g, almost double the amount present in the intact central cornea. These levels were transientin the central intact cornea only 2.08 µg/g could be detected at 2 h. Peak aqueous levels were low (0.95 ±. 24 µg/mL in debrided corneas at 1 h). The S/C injection of 1.500 µg of AmB in sodium deoxycholate produced a severe inflammatory response in the conjunctiva, episclera, iris, anterior chamber, and superior rectus muscle that persisted 10 days. Injection of sodium deoxycholate alone produced a similar but less severe response.