GDNF Family Receptor alpha1 Phenotype of Spermatogonial Stem Cells in Immature Mouse Testes1

Spermatogonial stem cells (SSCs) are essential for spermatogenesis, and these adult tissue stem cells balance self-renewal and differentiation to meet the biological demand of the testis. The developmental dynamics of SSCs are controlled, in part, by factors in the stem cell niche, which is located...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biology of reproduction 2005-11, Vol.73 (5), p.1011-1016
Hauptverfasser: Buageaw, Anyanee, Sukhwani, Meena, Ben-Yehudah, Ahmi, Ehmcke, Jens, Rawe, Vanesa Y, Pholpramool, Chumpol, Orwig, Kyle E, Schlatt, Stefan
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1016
container_issue 5
container_start_page 1011
container_title Biology of reproduction
container_volume 73
creator Buageaw, Anyanee
Sukhwani, Meena
Ben-Yehudah, Ahmi
Ehmcke, Jens
Rawe, Vanesa Y
Pholpramool, Chumpol
Orwig, Kyle E
Schlatt, Stefan
description Spermatogonial stem cells (SSCs) are essential for spermatogenesis, and these adult tissue stem cells balance self-renewal and differentiation to meet the biological demand of the testis. The developmental dynamics of SSCs are controlled, in part, by factors in the stem cell niche, which is located on the basement membrane of seminiferous tubules situated among Sertoli cells. Sertoli cells produce glial cell line-derived neurotrophic factor (GDNF), and disruption of GDNF expression results in spermatogenic defects and infertility. The GDNF signals through a receptor complex that includes GDNF family receptor α1 (GFRA1), which is thought to be expressed by SSCs. However, expression of GFRA1 on SSCs has not been confirmed by in vivo functional assay, which is the only method that allows definitive identification of SSCs. Therefore, we fractionated mouse pup testis cells based on GFRA1 expression using magnetic activated cell sorting. The sorted and depleted fractions of GFRA1 were characterized for germ cell markers by immunocytochemistry and for stem cell activity by germ cell transplantation. The GFRA1-positive cell fraction coeluted with other markers of SSCs, including ITGA6 and CD9, and was significantly depleted of KIT-positive cells. The transplantation results confirmed that a subpopulation of SSCs expresses GFRA1, but also that the stem cell pool is heterogeneous with respect to the level of GFRA1 expression. Interestingly, POU5F1-positive cells were enriched nearly 15-fold in the GFRA1-selected fraction, possibly suggesting heterogeneity of developmental potential within the stem cell pool.
doi_str_mv 10.1095/biolreprod.105.043810
format Article
fullrecord <record><control><sourceid>bioone_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1095_biolreprod_105_043810</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>bioone_primary_10_1095_biolreprod_105_043810</sourcerecordid><originalsourceid>FETCH-LOGICAL-b2550-db1470a78f31454a8594aa982f405cf37f64e5183776b885912e4b45a1cb9d9d3</originalsourceid><addsrcrecordid>eNqNkF9LwzAUR4MoOKcfQcgX6MxtkjZ9lGq3wfyDm29CSdsbV0mbknQP-_ZWJvjq0-XH4dyHQ8gtsAWwTN5VrbMeB--aacsFE1wBOyMzkHEWpXGizsmMMZZEnCf8klyF8MUYCB7zGflYPjwXtNBda4_0DWscRueptsNeA33dY-_G44DUGbod0Hd6dJ-ub7Wl2xE7mqO1gbY9XXcTOnikT-4QkO4wjBjgmlwYbQPe_N45eS8ed_kq2rws1_n9JqpiKVnUVCBSplNlOAgptJKZ0DpTsRFM1oanJhEoQfE0TSo1UYhRVEJqqKusyRo-J_L0t_YuBI-mHHzbaX8sgZU_icq_RNOW5SnR5PGTN2HX4z-tbyLmbYE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>GDNF Family Receptor alpha1 Phenotype of Spermatogonial Stem Cells in Immature Mouse Testes1</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>BioOne Complete</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Buageaw, Anyanee ; Sukhwani, Meena ; Ben-Yehudah, Ahmi ; Ehmcke, Jens ; Rawe, Vanesa Y ; Pholpramool, Chumpol ; Orwig, Kyle E ; Schlatt, Stefan</creator><creatorcontrib>Buageaw, Anyanee ; Sukhwani, Meena ; Ben-Yehudah, Ahmi ; Ehmcke, Jens ; Rawe, Vanesa Y ; Pholpramool, Chumpol ; Orwig, Kyle E ; Schlatt, Stefan</creatorcontrib><description>Spermatogonial stem cells (SSCs) are essential for spermatogenesis, and these adult tissue stem cells balance self-renewal and differentiation to meet the biological demand of the testis. The developmental dynamics of SSCs are controlled, in part, by factors in the stem cell niche, which is located on the basement membrane of seminiferous tubules situated among Sertoli cells. Sertoli cells produce glial cell line-derived neurotrophic factor (GDNF), and disruption of GDNF expression results in spermatogenic defects and infertility. The GDNF signals through a receptor complex that includes GDNF family receptor α1 (GFRA1), which is thought to be expressed by SSCs. However, expression of GFRA1 on SSCs has not been confirmed by in vivo functional assay, which is the only method that allows definitive identification of SSCs. Therefore, we fractionated mouse pup testis cells based on GFRA1 expression using magnetic activated cell sorting. The sorted and depleted fractions of GFRA1 were characterized for germ cell markers by immunocytochemistry and for stem cell activity by germ cell transplantation. The GFRA1-positive cell fraction coeluted with other markers of SSCs, including ITGA6 and CD9, and was significantly depleted of KIT-positive cells. The transplantation results confirmed that a subpopulation of SSCs expresses GFRA1, but also that the stem cell pool is heterogeneous with respect to the level of GFRA1 expression. Interestingly, POU5F1-positive cells were enriched nearly 15-fold in the GFRA1-selected fraction, possibly suggesting heterogeneity of developmental potential within the stem cell pool.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.105.043810</identifier><language>eng</language><subject>CONTENTS ; gamete biology ; spermatogenesis ; testis</subject><ispartof>Biology of reproduction, 2005-11, Vol.73 (5), p.1011-1016</ispartof><rights>Society for the Study of Reproduction</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b2550-db1470a78f31454a8594aa982f405cf37f64e5183776b885912e4b45a1cb9d9d3</citedby><cites>FETCH-LOGICAL-b2550-db1470a78f31454a8594aa982f405cf37f64e5183776b885912e4b45a1cb9d9d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1095/biolreprod.105.043810$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>314,780,784,26978,27924,27925,52363</link.rule.ids></links><search><creatorcontrib>Buageaw, Anyanee</creatorcontrib><creatorcontrib>Sukhwani, Meena</creatorcontrib><creatorcontrib>Ben-Yehudah, Ahmi</creatorcontrib><creatorcontrib>Ehmcke, Jens</creatorcontrib><creatorcontrib>Rawe, Vanesa Y</creatorcontrib><creatorcontrib>Pholpramool, Chumpol</creatorcontrib><creatorcontrib>Orwig, Kyle E</creatorcontrib><creatorcontrib>Schlatt, Stefan</creatorcontrib><title>GDNF Family Receptor alpha1 Phenotype of Spermatogonial Stem Cells in Immature Mouse Testes1</title><title>Biology of reproduction</title><description>Spermatogonial stem cells (SSCs) are essential for spermatogenesis, and these adult tissue stem cells balance self-renewal and differentiation to meet the biological demand of the testis. The developmental dynamics of SSCs are controlled, in part, by factors in the stem cell niche, which is located on the basement membrane of seminiferous tubules situated among Sertoli cells. Sertoli cells produce glial cell line-derived neurotrophic factor (GDNF), and disruption of GDNF expression results in spermatogenic defects and infertility. The GDNF signals through a receptor complex that includes GDNF family receptor α1 (GFRA1), which is thought to be expressed by SSCs. However, expression of GFRA1 on SSCs has not been confirmed by in vivo functional assay, which is the only method that allows definitive identification of SSCs. Therefore, we fractionated mouse pup testis cells based on GFRA1 expression using magnetic activated cell sorting. The sorted and depleted fractions of GFRA1 were characterized for germ cell markers by immunocytochemistry and for stem cell activity by germ cell transplantation. The GFRA1-positive cell fraction coeluted with other markers of SSCs, including ITGA6 and CD9, and was significantly depleted of KIT-positive cells. The transplantation results confirmed that a subpopulation of SSCs expresses GFRA1, but also that the stem cell pool is heterogeneous with respect to the level of GFRA1 expression. Interestingly, POU5F1-positive cells were enriched nearly 15-fold in the GFRA1-selected fraction, possibly suggesting heterogeneity of developmental potential within the stem cell pool.</description><subject>CONTENTS</subject><subject>gamete biology</subject><subject>spermatogenesis</subject><subject>testis</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqNkF9LwzAUR4MoOKcfQcgX6MxtkjZ9lGq3wfyDm29CSdsbV0mbknQP-_ZWJvjq0-XH4dyHQ8gtsAWwTN5VrbMeB--aacsFE1wBOyMzkHEWpXGizsmMMZZEnCf8klyF8MUYCB7zGflYPjwXtNBda4_0DWscRueptsNeA33dY-_G44DUGbod0Hd6dJ-ub7Wl2xE7mqO1gbY9XXcTOnikT-4QkO4wjBjgmlwYbQPe_N45eS8ed_kq2rws1_n9JqpiKVnUVCBSplNlOAgptJKZ0DpTsRFM1oanJhEoQfE0TSo1UYhRVEJqqKusyRo-J_L0t_YuBI-mHHzbaX8sgZU_icq_RNOW5SnR5PGTN2HX4z-tbyLmbYE</recordid><startdate>200511</startdate><enddate>200511</enddate><creator>Buageaw, Anyanee</creator><creator>Sukhwani, Meena</creator><creator>Ben-Yehudah, Ahmi</creator><creator>Ehmcke, Jens</creator><creator>Rawe, Vanesa Y</creator><creator>Pholpramool, Chumpol</creator><creator>Orwig, Kyle E</creator><creator>Schlatt, Stefan</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>200511</creationdate><title>GDNF Family Receptor alpha1 Phenotype of Spermatogonial Stem Cells in Immature Mouse Testes1</title><author>Buageaw, Anyanee ; Sukhwani, Meena ; Ben-Yehudah, Ahmi ; Ehmcke, Jens ; Rawe, Vanesa Y ; Pholpramool, Chumpol ; Orwig, Kyle E ; Schlatt, Stefan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b2550-db1470a78f31454a8594aa982f405cf37f64e5183776b885912e4b45a1cb9d9d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>CONTENTS</topic><topic>gamete biology</topic><topic>spermatogenesis</topic><topic>testis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Buageaw, Anyanee</creatorcontrib><creatorcontrib>Sukhwani, Meena</creatorcontrib><creatorcontrib>Ben-Yehudah, Ahmi</creatorcontrib><creatorcontrib>Ehmcke, Jens</creatorcontrib><creatorcontrib>Rawe, Vanesa Y</creatorcontrib><creatorcontrib>Pholpramool, Chumpol</creatorcontrib><creatorcontrib>Orwig, Kyle E</creatorcontrib><creatorcontrib>Schlatt, Stefan</creatorcontrib><collection>CrossRef</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Buageaw, Anyanee</au><au>Sukhwani, Meena</au><au>Ben-Yehudah, Ahmi</au><au>Ehmcke, Jens</au><au>Rawe, Vanesa Y</au><au>Pholpramool, Chumpol</au><au>Orwig, Kyle E</au><au>Schlatt, Stefan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GDNF Family Receptor alpha1 Phenotype of Spermatogonial Stem Cells in Immature Mouse Testes1</atitle><jtitle>Biology of reproduction</jtitle><date>2005-11</date><risdate>2005</risdate><volume>73</volume><issue>5</issue><spage>1011</spage><epage>1016</epage><pages>1011-1016</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><abstract>Spermatogonial stem cells (SSCs) are essential for spermatogenesis, and these adult tissue stem cells balance self-renewal and differentiation to meet the biological demand of the testis. The developmental dynamics of SSCs are controlled, in part, by factors in the stem cell niche, which is located on the basement membrane of seminiferous tubules situated among Sertoli cells. Sertoli cells produce glial cell line-derived neurotrophic factor (GDNF), and disruption of GDNF expression results in spermatogenic defects and infertility. The GDNF signals through a receptor complex that includes GDNF family receptor α1 (GFRA1), which is thought to be expressed by SSCs. However, expression of GFRA1 on SSCs has not been confirmed by in vivo functional assay, which is the only method that allows definitive identification of SSCs. Therefore, we fractionated mouse pup testis cells based on GFRA1 expression using magnetic activated cell sorting. The sorted and depleted fractions of GFRA1 were characterized for germ cell markers by immunocytochemistry and for stem cell activity by germ cell transplantation. The GFRA1-positive cell fraction coeluted with other markers of SSCs, including ITGA6 and CD9, and was significantly depleted of KIT-positive cells. The transplantation results confirmed that a subpopulation of SSCs expresses GFRA1, but also that the stem cell pool is heterogeneous with respect to the level of GFRA1 expression. Interestingly, POU5F1-positive cells were enriched nearly 15-fold in the GFRA1-selected fraction, possibly suggesting heterogeneity of developmental potential within the stem cell pool.</abstract><doi>10.1095/biolreprod.105.043810</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0006-3363
ispartof Biology of reproduction, 2005-11, Vol.73 (5), p.1011-1016
issn 0006-3363
1529-7268
language eng
recordid cdi_crossref_primary_10_1095_biolreprod_105_043810
source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; BioOne Complete; Oxford University Press Journals All Titles (1996-Current)
subjects CONTENTS
gamete biology
spermatogenesis
testis
title GDNF Family Receptor alpha1 Phenotype of Spermatogonial Stem Cells in Immature Mouse Testes1
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T16%3A41%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-bioone_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=GDNF%20Family%20Receptor%20alpha1%20Phenotype%20of%20Spermatogonial%20Stem%20Cells%20in%20Immature%20Mouse%20Testes1&rft.jtitle=Biology%20of%20reproduction&rft.au=Buageaw,%20Anyanee&rft.date=2005-11&rft.volume=73&rft.issue=5&rft.spage=1011&rft.epage=1016&rft.pages=1011-1016&rft.issn=0006-3363&rft.eissn=1529-7268&rft_id=info:doi/10.1095/biolreprod.105.043810&rft_dat=%3Cbioone_cross%3Ebioone_primary_10_1095_biolreprod_105_043810%3C/bioone_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true