Quantitative Analysis of Gene Expression in Preimplantation Mouse Embryos Using Green Fluorescent Protein Reporter1
We have developed a method to monitor noninvasively, quantitatively, and in real-time transcription in living preimplantation mouse embryos by measuring expression of a short half-life form of enhanced green fluoresecent protein (EGFP) following microinjection of a plasmid-borne EGFP reporter gene....
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| Veröffentlicht in: | Biology of reproduction 2002-07, Vol.67 (1), p.282-286 |
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| container_issue | 1 |
| container_start_page | 282 |
| container_title | Biology of reproduction |
| container_volume | 67 |
| creator | Medvedev, Serguei Yu Tokunaga, Tomoyuki Schultz, Richard M Furukawa, Tsutomu Nagai, Takashi Yamaguchi, Manabu Hosoe, Misa Yakovlev, Alexander F Takahashi, Seiya Izaike, Yoshiaki |
| description | We have developed a method to monitor noninvasively, quantitatively, and in real-time transcription in living preimplantation mouse embryos by measuring expression of a short half-life form of enhanced green fluoresecent protein (EGFP) following microinjection of a plasmid-borne EGFP reporter gene. A standard curve was established by injecting known amounts of recombinant green fluorescent protein, and transcriptional activity was then determined by interpolating the amount of fluoresence in the DNA-injected embryos. This approach permitted multiple measurements in single embryos with no significant detrimental effect on embryonic development as long as light exposure was brief ( |
| doi_str_mv | 10.1095/biolreprod67.1.282 |
| format | Article |
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| identifier | ISSN: 0006-3363 |
| ispartof | Biology of reproduction, 2002-07, Vol.67 (1), p.282-286 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_crossref_primary_10_1095_biolreprod67_1_282 |
| source | Oxford University Press Journals All Titles (1996-Current); Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; BioOne Complete |
| subjects | Contents developmental biology early development embryo gene regulation |
| title | Quantitative Analysis of Gene Expression in Preimplantation Mouse Embryos Using Green Fluorescent Protein Reporter1 |
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