Identification of a randomly amplified polymorphic DNA marker linked to the Fom 2 Fusarium wilt resistance gene in muskmelon MR-1
Resistance to Fusarium oxysporum f. sp. melonis race 1 (causal agent of Fusarium wilt) in Cucumis melo (muskmelon) is controlled by the dominant gene Fom 2. Bulked segregant and random amplified polymorphic DNA (RAPD) analyses were employed to identify genetic markers linked to race 1 Fusarium wilt...
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| Veröffentlicht in: | Phytopathology 1995-10, Vol.85 (10), p.1245-1249 |
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| creator | Wechter, W.P. (Clemson University, Clemson, SC.) Whitehead, M.P Thomas, C.E Dean, R.A |
| description | Resistance to Fusarium oxysporum f. sp. melonis race 1 (causal agent of Fusarium wilt) in Cucumis melo (muskmelon) is controlled by the dominant gene Fom 2. Bulked segregant and random amplified polymorphic DNA (RAPD) analyses were employed to identify genetic markers linked to race 1 Fusarium wilt resistance. DNA was isolated from pooled (bulked) susceptible or resistant plant tissue from an F2 population of a susceptible AY X resistant MR-1 cross that segregated for race 1 resistance. Bulks from resistant plants were composed of both homozygous and heterozygous individuals and also pure homozygous individuals, determined by analysis of F3 populations. After screening the DNA from bulked resistant and susceptible plants by PCR with 320 decamer primers, one primer, 5' CCC CTC GAA T 3', produced a 1.6-kb fragment in the resistant bulks that was absent from the susceptible bulks. Using this primer to screen individual F2 plants, the outcome of 92 of 94 individual host-pathogen interactions was correctly predicted. Nine susceptible and three resistant commercial cultivars also were screened with this primer. The 1.6-kb fragment was not amplified using DNA from any of these cultivars. This is the first report of a RAPD marker linked to resistance to Fusarium wilt in muskmelon |
| doi_str_mv | 10.1094/Phyto-85-1245 |
| format | Article |
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(Clemson University, Clemson, SC.) ; Whitehead, M.P ; Thomas, C.E ; Dean, R.A</creator><creatorcontrib>Wechter, W.P. (Clemson University, Clemson, SC.) ; Whitehead, M.P ; Thomas, C.E ; Dean, R.A</creatorcontrib><description>Resistance to Fusarium oxysporum f. sp. melonis race 1 (causal agent of Fusarium wilt) in Cucumis melo (muskmelon) is controlled by the dominant gene Fom 2. Bulked segregant and random amplified polymorphic DNA (RAPD) analyses were employed to identify genetic markers linked to race 1 Fusarium wilt resistance. DNA was isolated from pooled (bulked) susceptible or resistant plant tissue from an F2 population of a susceptible AY X resistant MR-1 cross that segregated for race 1 resistance. Bulks from resistant plants were composed of both homozygous and heterozygous individuals and also pure homozygous individuals, determined by analysis of F3 populations. After screening the DNA from bulked resistant and susceptible plants by PCR with 320 decamer primers, one primer, 5' CCC CTC GAA T 3', produced a 1.6-kb fragment in the resistant bulks that was absent from the susceptible bulks. Using this primer to screen individual F2 plants, the outcome of 92 of 94 individual host-pathogen interactions was correctly predicted. Nine susceptible and three resistant commercial cultivars also were screened with this primer. The 1.6-kb fragment was not amplified using DNA from any of these cultivars. This is the first report of a RAPD marker linked to resistance to Fusarium wilt in muskmelon</description><identifier>ISSN: 0031-949X</identifier><identifier>EISSN: 1943-7684</identifier><identifier>DOI: 10.1094/Phyto-85-1245</identifier><identifier>CODEN: PHYTAJ</identifier><language>eng</language><publisher>St. Paul, MN: American Phytopathological Society</publisher><subject>ADN ; Agronomy. Soil science and plant productions ; AMELIORATION DES PLANTES ; Biological and medical sciences ; CUCUMIS MELO ; FITOMEJORAMIENTO ; Fundamental and applied biological sciences. Psychology ; FUSARIUM OXYSPORUM ; GENE ; GENES ; GENETICA ; Genetics and breeding of economic plants ; GENETIQUE ; MARCADORES GENETICOS ; MARQUEUR GENETIQUE ; Pest resistance ; Plant pathogens ; POLIMORFISMO GENETICO ; POLYMORPHISME GENETIQUE ; RESISTANCE AUX MALADIES ; RESISTENCIA A LA ENFERMEDAD ; SELECCION ; SELECTION ; VARIACION GENETICA ; VARIATION GENETIQUE ; VARIEDADES ; Varietal selection. Specialized plant breeding, plant breeding aims ; VARIETE</subject><ispartof>Phytopathology, 1995-10, Vol.85 (10), p.1245-1249</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c283t-bf4d76f1269782cf2279230258982e3144b27e2b5e734af8e04126457c6543f13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,777,781,786,787,3711,23911,23912,25121,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3698415$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Wechter, W.P. (Clemson University, Clemson, SC.)</creatorcontrib><creatorcontrib>Whitehead, M.P</creatorcontrib><creatorcontrib>Thomas, C.E</creatorcontrib><creatorcontrib>Dean, R.A</creatorcontrib><title>Identification of a randomly amplified polymorphic DNA marker linked to the Fom 2 Fusarium wilt resistance gene in muskmelon MR-1</title><title>Phytopathology</title><description>Resistance to Fusarium oxysporum f. sp. melonis race 1 (causal agent of Fusarium wilt) in Cucumis melo (muskmelon) is controlled by the dominant gene Fom 2. Bulked segregant and random amplified polymorphic DNA (RAPD) analyses were employed to identify genetic markers linked to race 1 Fusarium wilt resistance. DNA was isolated from pooled (bulked) susceptible or resistant plant tissue from an F2 population of a susceptible AY X resistant MR-1 cross that segregated for race 1 resistance. Bulks from resistant plants were composed of both homozygous and heterozygous individuals and also pure homozygous individuals, determined by analysis of F3 populations. After screening the DNA from bulked resistant and susceptible plants by PCR with 320 decamer primers, one primer, 5' CCC CTC GAA T 3', produced a 1.6-kb fragment in the resistant bulks that was absent from the susceptible bulks. Using this primer to screen individual F2 plants, the outcome of 92 of 94 individual host-pathogen interactions was correctly predicted. Nine susceptible and three resistant commercial cultivars also were screened with this primer. The 1.6-kb fragment was not amplified using DNA from any of these cultivars. This is the first report of a RAPD marker linked to resistance to Fusarium wilt in muskmelon</description><subject>ADN</subject><subject>Agronomy. Soil science and plant productions</subject><subject>AMELIORATION DES PLANTES</subject><subject>Biological and medical sciences</subject><subject>CUCUMIS MELO</subject><subject>FITOMEJORAMIENTO</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>FUSARIUM OXYSPORUM</subject><subject>GENE</subject><subject>GENES</subject><subject>GENETICA</subject><subject>Genetics and breeding of economic plants</subject><subject>GENETIQUE</subject><subject>MARCADORES GENETICOS</subject><subject>MARQUEUR GENETIQUE</subject><subject>Pest resistance</subject><subject>Plant pathogens</subject><subject>POLIMORFISMO GENETICO</subject><subject>POLYMORPHISME GENETIQUE</subject><subject>RESISTANCE AUX MALADIES</subject><subject>RESISTENCIA A LA ENFERMEDAD</subject><subject>SELECCION</subject><subject>SELECTION</subject><subject>VARIACION GENETICA</subject><subject>VARIATION GENETIQUE</subject><subject>VARIEDADES</subject><subject>Varietal selection. 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(Clemson University, Clemson, SC.)</creator><creator>Whitehead, M.P</creator><creator>Thomas, C.E</creator><creator>Dean, R.A</creator><general>American Phytopathological Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19951001</creationdate><title>Identification of a randomly amplified polymorphic DNA marker linked to the Fom 2 Fusarium wilt resistance gene in muskmelon MR-1</title><author>Wechter, W.P. (Clemson University, Clemson, SC.) ; Whitehead, M.P ; Thomas, C.E ; Dean, R.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c283t-bf4d76f1269782cf2279230258982e3144b27e2b5e734af8e04126457c6543f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>ADN</topic><topic>Agronomy. Soil science and plant productions</topic><topic>AMELIORATION DES PLANTES</topic><topic>Biological and medical sciences</topic><topic>CUCUMIS MELO</topic><topic>FITOMEJORAMIENTO</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>FUSARIUM OXYSPORUM</topic><topic>GENE</topic><topic>GENES</topic><topic>GENETICA</topic><topic>Genetics and breeding of economic plants</topic><topic>GENETIQUE</topic><topic>MARCADORES GENETICOS</topic><topic>MARQUEUR GENETIQUE</topic><topic>Pest resistance</topic><topic>Plant pathogens</topic><topic>POLIMORFISMO GENETICO</topic><topic>POLYMORPHISME GENETIQUE</topic><topic>RESISTANCE AUX MALADIES</topic><topic>RESISTENCIA A LA ENFERMEDAD</topic><topic>SELECCION</topic><topic>SELECTION</topic><topic>VARIACION GENETICA</topic><topic>VARIATION GENETIQUE</topic><topic>VARIEDADES</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><topic>VARIETE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wechter, W.P. (Clemson University, Clemson, SC.)</creatorcontrib><creatorcontrib>Whitehead, M.P</creatorcontrib><creatorcontrib>Thomas, C.E</creatorcontrib><creatorcontrib>Dean, R.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Phytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wechter, W.P. (Clemson University, Clemson, SC.)</au><au>Whitehead, M.P</au><au>Thomas, C.E</au><au>Dean, R.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a randomly amplified polymorphic DNA marker linked to the Fom 2 Fusarium wilt resistance gene in muskmelon MR-1</atitle><jtitle>Phytopathology</jtitle><date>1995-10-01</date><risdate>1995</risdate><volume>85</volume><issue>10</issue><spage>1245</spage><epage>1249</epage><pages>1245-1249</pages><issn>0031-949X</issn><eissn>1943-7684</eissn><coden>PHYTAJ</coden><abstract>Resistance to Fusarium oxysporum f. sp. melonis race 1 (causal agent of Fusarium wilt) in Cucumis melo (muskmelon) is controlled by the dominant gene Fom 2. Bulked segregant and random amplified polymorphic DNA (RAPD) analyses were employed to identify genetic markers linked to race 1 Fusarium wilt resistance. DNA was isolated from pooled (bulked) susceptible or resistant plant tissue from an F2 population of a susceptible AY X resistant MR-1 cross that segregated for race 1 resistance. Bulks from resistant plants were composed of both homozygous and heterozygous individuals and also pure homozygous individuals, determined by analysis of F3 populations. After screening the DNA from bulked resistant and susceptible plants by PCR with 320 decamer primers, one primer, 5' CCC CTC GAA T 3', produced a 1.6-kb fragment in the resistant bulks that was absent from the susceptible bulks. Using this primer to screen individual F2 plants, the outcome of 92 of 94 individual host-pathogen interactions was correctly predicted. Nine susceptible and three resistant commercial cultivars also were screened with this primer. The 1.6-kb fragment was not amplified using DNA from any of these cultivars. This is the first report of a RAPD marker linked to resistance to Fusarium wilt in muskmelon</abstract><cop>St. Paul, MN</cop><pub>American Phytopathological Society</pub><doi>10.1094/Phyto-85-1245</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-949X |
| ispartof | Phytopathology, 1995-10, Vol.85 (10), p.1245-1249 |
| issn | 0031-949X 1943-7684 |
| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; American Phytopathological Society Journal Back Issues |
| subjects | ADN Agronomy. Soil science and plant productions AMELIORATION DES PLANTES Biological and medical sciences CUCUMIS MELO FITOMEJORAMIENTO Fundamental and applied biological sciences. Psychology FUSARIUM OXYSPORUM GENE GENES GENETICA Genetics and breeding of economic plants GENETIQUE MARCADORES GENETICOS MARQUEUR GENETIQUE Pest resistance Plant pathogens POLIMORFISMO GENETICO POLYMORPHISME GENETIQUE RESISTANCE AUX MALADIES RESISTENCIA A LA ENFERMEDAD SELECCION SELECTION VARIACION GENETICA VARIATION GENETIQUE VARIEDADES Varietal selection. Specialized plant breeding, plant breeding aims VARIETE |
| title | Identification of a randomly amplified polymorphic DNA marker linked to the Fom 2 Fusarium wilt resistance gene in muskmelon MR-1 |
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