A biochemical method for estimating viability of teliospores of Tilletia controversa
A rapid biochemical test was developed to assess indirectly the viability of teliospores of Tilletia controversa, the cause of dwarf bunt of wheat, that contaminate wheat grain. Lipase activity was detected consistently in extracts from viable teliospores by a fluorescein diacetate (FDA) assay. No f...
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| Veröffentlicht in: | Phytopathology 1990-05, Vol.80 (5), p.474-476 |
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| container_end_page | 476 |
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| container_title | Phytopathology |
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| creator | Chastain, T.G. (Oregon State University, Pendleton) King, B |
| description | A rapid biochemical test was developed to assess indirectly the viability of teliospores of Tilletia controversa, the cause of dwarf bunt of wheat, that contaminate wheat grain. Lipase activity was detected consistently in extracts from viable teliospores by a fluorescein diacetate (FDA) assay. No fluorescence activity was observed in extracts from autoclaved spores. By comparison, lipase detection was inconsistent when 4-methyl- umbelliferyl-palmitate was used as substrate. Extracts of teliospores that did not germinate on soil extract agar exhibited no lipase or glucosidase activity. Viability of individual teliospores could not be assessed by lipase assay because spore coats were impermeable to FDA. The FDA assay reduced the time needed to assess the average viability of a teliospore population from 2 mo to 1 hr |
| doi_str_mv | 10.1094/Phyto-80-474 |
| format | Article |
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(Oregon State University, Pendleton) ; King, B</creator><creatorcontrib>Chastain, T.G. (Oregon State University, Pendleton) ; King, B</creatorcontrib><description>A rapid biochemical test was developed to assess indirectly the viability of teliospores of Tilletia controversa, the cause of dwarf bunt of wheat, that contaminate wheat grain. Lipase activity was detected consistently in extracts from viable teliospores by a fluorescein diacetate (FDA) assay. No fluorescence activity was observed in extracts from autoclaved spores. By comparison, lipase detection was inconsistent when 4-methyl- umbelliferyl-palmitate was used as substrate. Extracts of teliospores that did not germinate on soil extract agar exhibited no lipase or glucosidase activity. Viability of individual teliospores could not be assessed by lipase assay because spore coats were impermeable to FDA. The FDA assay reduced the time needed to assess the average viability of a teliospore population from 2 mo to 1 hr</description><identifier>ISSN: 0031-949X</identifier><identifier>EISSN: 1943-7684</identifier><identifier>DOI: 10.1094/Phyto-80-474</identifier><identifier>CODEN: PHYTAJ</identifier><language>eng</language><publisher>St. Paul, MN: American Phytopathological Society</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; Agronomy. Soil science and plant productions ; BIOCHIMIE ; Biological and medical sciences ; BIOQUIMICA ; ESPORAS ; ESSAI ; FACULTE GERMINATIVE ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; GLUCOSIDASA ; GLUCOSIDASE ; METHODE ; METODOS ; Pathology, epidemiology, host-fungus relationships. Damages, economic importance ; Phytopathology. Animal pests. Plant and forest protection ; PODER GERMINATIVO ; PROPIEDADES OPTICAS ; PROPRIETE OPTIQUE ; PRUEBAS ; SPORE ; TILLETIA ; TRIACILGLICEROL LIPASA ; TRIACYLGLYCEROL LIPASE ; TRITICUM AESTIVUM</subject><ispartof>Phytopathology, 1990-05, Vol.80 (5), p.474-476</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c214t-a62522c1c82552c3c435c8b37a09119ec17b4d6dab4e167c3c9a46d4ff25e29a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,3713,27907,27908</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19350083$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Chastain, T.G. 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The FDA assay reduced the time needed to assess the average viability of a teliospore population from 2 mo to 1 hr</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>Agronomy. Soil science and plant productions</subject><subject>BIOCHIMIE</subject><subject>Biological and medical sciences</subject><subject>BIOQUIMICA</subject><subject>ESPORAS</subject><subject>ESSAI</subject><subject>FACULTE GERMINATIVE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>GLUCOSIDASA</subject><subject>GLUCOSIDASE</subject><subject>METHODE</subject><subject>METODOS</subject><subject>Pathology, epidemiology, host-fungus relationships. Damages, economic importance</subject><subject>Phytopathology. Animal pests. 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Soil science and plant productions</topic><topic>BIOCHIMIE</topic><topic>Biological and medical sciences</topic><topic>BIOQUIMICA</topic><topic>ESPORAS</topic><topic>ESSAI</topic><topic>FACULTE GERMINATIVE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>GLUCOSIDASA</topic><topic>GLUCOSIDASE</topic><topic>METHODE</topic><topic>METODOS</topic><topic>Pathology, epidemiology, host-fungus relationships. Damages, economic importance</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>PODER GERMINATIVO</topic><topic>PROPIEDADES OPTICAS</topic><topic>PROPRIETE OPTIQUE</topic><topic>PRUEBAS</topic><topic>SPORE</topic><topic>TILLETIA</topic><topic>TRIACILGLICEROL LIPASA</topic><topic>TRIACYLGLYCEROL LIPASE</topic><topic>TRITICUM AESTIVUM</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chastain, T.G. (Oregon State University, Pendleton)</creatorcontrib><creatorcontrib>King, B</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Phytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chastain, T.G. (Oregon State University, Pendleton)</au><au>King, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A biochemical method for estimating viability of teliospores of Tilletia controversa</atitle><jtitle>Phytopathology</jtitle><date>1990-05-01</date><risdate>1990</risdate><volume>80</volume><issue>5</issue><spage>474</spage><epage>476</epage><pages>474-476</pages><issn>0031-949X</issn><eissn>1943-7684</eissn><coden>PHYTAJ</coden><abstract>A rapid biochemical test was developed to assess indirectly the viability of teliospores of Tilletia controversa, the cause of dwarf bunt of wheat, that contaminate wheat grain. Lipase activity was detected consistently in extracts from viable teliospores by a fluorescein diacetate (FDA) assay. No fluorescence activity was observed in extracts from autoclaved spores. By comparison, lipase detection was inconsistent when 4-methyl- umbelliferyl-palmitate was used as substrate. Extracts of teliospores that did not germinate on soil extract agar exhibited no lipase or glucosidase activity. Viability of individual teliospores could not be assessed by lipase assay because spore coats were impermeable to FDA. The FDA assay reduced the time needed to assess the average viability of a teliospore population from 2 mo to 1 hr</abstract><cop>St. Paul, MN</cop><pub>American Phytopathological Society</pub><doi>10.1094/Phyto-80-474</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-949X |
| ispartof | Phytopathology, 1990-05, Vol.80 (5), p.474-476 |
| issn | 0031-949X 1943-7684 |
| language | eng |
| recordid | cdi_crossref_primary_10_1094_Phyto_80_474 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; American Phytopathological Society Journal Back Issues |
| subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Agronomy. Soil science and plant productions BIOCHIMIE Biological and medical sciences BIOQUIMICA ESPORAS ESSAI FACULTE GERMINATIVE Fundamental and applied biological sciences. Psychology Fungal plant pathogens GLUCOSIDASA GLUCOSIDASE METHODE METODOS Pathology, epidemiology, host-fungus relationships. Damages, economic importance Phytopathology. Animal pests. Plant and forest protection PODER GERMINATIVO PROPIEDADES OPTICAS PROPRIETE OPTIQUE PRUEBAS SPORE TILLETIA TRIACILGLICEROL LIPASA TRIACYLGLYCEROL LIPASE TRITICUM AESTIVUM |
| title | A biochemical method for estimating viability of teliospores of Tilletia controversa |
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