Synthetic single-framework antibody library integrated with rapid affinity maturation by VL shuffling

Affinity maturation is often applied to improve the properties of antibodies isolated from universal antibody libraries in vitro. A synthetic human scFv antibody library was constructed in single immunoglobulin framework to enable rapid affinity maturation by updated Kunkel's mutagenesis. The i...

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Veröffentlicht in:	Protein engineering, design and selection design and selection, 2011-09, Vol.24 (9), p.691-700
Hauptverfasser:	Brockmann, E.-C., Akter, S., Savukoski, T., Huovinen, T., Lehmusvuori, A., Leivo, J., Saavalainen, O., Azhayev, A., Lövgren, T., Hellman, J., Lamminmäki, U.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Antibody Affinity - genetics DNA Shuffling - methods Humans Molecular Sequence Data Muramidase - genetics Muramidase - immunology Muramidase - metabolism Mutagenesis Peptide Library Protein Engineering - methods Protein Folding Reproducibility of Results Single-Chain Antibodies - genetics Single-Chain Antibodies - immunology Single-Chain Antibodies - metabolism
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