Specific detection of PCR product from Legionella pneumophila strainPhiladelphia1 using zinc finger protein Sp2

Specific detection of PCR product from Legionella pneumophila strainPhiladelphia1 using zinc finger protein Sp2

We have developed a novel method to detect PCR products from pathogen genome DNA by Zinc finger protein that can bind to double strand DNA (dsDNA) in sequence specific manner. In this study, we tried to detect Legionella pneumophila strain Philadelphia 1 using Zinc finger protein. We found the speci...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nucleic Acids Symposium Series 2007-11, Vol.51 (1), p.285-286
Hauptverfasser: Ikebukuro, Kazunori, Kumagai, Takenori, Motoki, Hiroaki, Osawa, Yuko, Matuo, Takafumi, Horiuchi, Michio, Sode, Koji
Format: Artikel
Sprache:eng
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 286
container_issue 1
container_start_page 285
container_title Nucleic Acids Symposium Series
container_volume 51
creator Ikebukuro, Kazunori
Kumagai, Takenori
Motoki, Hiroaki
Osawa, Yuko
Matuo, Takafumi
Horiuchi, Michio
Sode, Koji
description We have developed a novel method to detect PCR products from pathogen genome DNA by Zinc finger protein that can bind to double strand DNA (dsDNA) in sequence specific manner. In this study, we tried to detect Legionella pneumophila strain Philadelphia 1 using Zinc finger protein. We found the specific target DNA sequence for zinc finger protein Sp2 in L. pneumophila strain Philadelphia 1 genome DNA. Specific PCR product was successfully amplified from L. pneumophila strain Philadelphia 1 genome DNA and we used Zinc finger protein Sp2 to detect it. We succeeded in detecting the PCR products from L. pneumophila strain Philadelphia 1 genome DNA with Sp2.
doi_str_mv 10.1093/nass/nrm143
format Article
fullrecord <record><control><sourceid>oup_TOX</sourceid><recordid>TN_cdi_crossref_primary_10_1093_nass_nrm143</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/nass/nrm143</oup_id><sourcerecordid>10.1093/nass/nrm143</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1043-e79d257851f93da1328e18ac52257d820e3298f96dd999cc0c2274d873ece03c3</originalsourceid><addsrcrecordid>eNp9kEtLAzEUhYMoWGpX_oGs3MjYPKaTZCnFFxQsVtdDSG5qZCYJycxCf70z1LV3cw_nfJzFQeiakjtKFF8HXco65J7W_AwtqKibSjLBztGCsIZWnDbNJVqV8kWmqwkRSixQPCQw3nmDLQxgBh8Djg7vt2845WhHM2CXY493cJwi6DqNU4Cxj-nTT7oMWfuwn7WFbvI0xWPx4Yh_fDDYTQry3DSAD_iQ2BW6cLorsPr7S_Tx-PC-fa52r08v2_tdZSipeQVCWbYRckOd4lZTziRQqc2GTa6VjABnSjrVWKuUMoYYxkRtpeBggHDDl-j21GtyLCWDa1P2vc7fLSXtPFc7z9We5promxMdx_Qv-As9U21O</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Specific detection of PCR product from Legionella pneumophila strainPhiladelphia1 using zinc finger protein Sp2</title><source>Oxford Journals Open Access Collection</source><creator>Ikebukuro, Kazunori ; Kumagai, Takenori ; Motoki, Hiroaki ; Osawa, Yuko ; Matuo, Takafumi ; Horiuchi, Michio ; Sode, Koji</creator><creatorcontrib>Ikebukuro, Kazunori ; Kumagai, Takenori ; Motoki, Hiroaki ; Osawa, Yuko ; Matuo, Takafumi ; Horiuchi, Michio ; Sode, Koji</creatorcontrib><description>We have developed a novel method to detect PCR products from pathogen genome DNA by Zinc finger protein that can bind to double strand DNA (dsDNA) in sequence specific manner. In this study, we tried to detect Legionella pneumophila strain Philadelphia 1 using Zinc finger protein. We found the specific target DNA sequence for zinc finger protein Sp2 in L. pneumophila strain Philadelphia 1 genome DNA. Specific PCR product was successfully amplified from L. pneumophila strain Philadelphia 1 genome DNA and we used Zinc finger protein Sp2 to detect it. We succeeded in detecting the PCR products from L. pneumophila strain Philadelphia 1 genome DNA with Sp2.</description><identifier>ISSN: 0261-3166</identifier><identifier>EISSN: 1746-8272</identifier><identifier>DOI: 10.1093/nass/nrm143</identifier><language>eng</language><publisher>Oxford University Press</publisher><ispartof>Nucleic Acids Symposium Series, 2007-11, Vol.51 (1), p.285-286</ispartof><rights>2007 Oxford University Press 2007</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,1605,27928,27929</link.rule.ids><linktorsrc>$$Uhttps://dx.doi.org/10.1093/nass/nrm143$$EView_record_in_Oxford_University_Press$$FView_record_in_$$GOxford_University_Press</linktorsrc></links><search><creatorcontrib>Ikebukuro, Kazunori</creatorcontrib><creatorcontrib>Kumagai, Takenori</creatorcontrib><creatorcontrib>Motoki, Hiroaki</creatorcontrib><creatorcontrib>Osawa, Yuko</creatorcontrib><creatorcontrib>Matuo, Takafumi</creatorcontrib><creatorcontrib>Horiuchi, Michio</creatorcontrib><creatorcontrib>Sode, Koji</creatorcontrib><title>Specific detection of PCR product from Legionella pneumophila strainPhiladelphia1 using zinc finger protein Sp2</title><title>Nucleic Acids Symposium Series</title><description>We have developed a novel method to detect PCR products from pathogen genome DNA by Zinc finger protein that can bind to double strand DNA (dsDNA) in sequence specific manner. In this study, we tried to detect Legionella pneumophila strain Philadelphia 1 using Zinc finger protein. We found the specific target DNA sequence for zinc finger protein Sp2 in L. pneumophila strain Philadelphia 1 genome DNA. Specific PCR product was successfully amplified from L. pneumophila strain Philadelphia 1 genome DNA and we used Zinc finger protein Sp2 to detect it. We succeeded in detecting the PCR products from L. pneumophila strain Philadelphia 1 genome DNA with Sp2.</description><issn>0261-3166</issn><issn>1746-8272</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLAzEUhYMoWGpX_oGs3MjYPKaTZCnFFxQsVtdDSG5qZCYJycxCf70z1LV3cw_nfJzFQeiakjtKFF8HXco65J7W_AwtqKibSjLBztGCsIZWnDbNJVqV8kWmqwkRSixQPCQw3nmDLQxgBh8Djg7vt2845WhHM2CXY493cJwi6DqNU4Cxj-nTT7oMWfuwn7WFbvI0xWPx4Yh_fDDYTQry3DSAD_iQ2BW6cLorsPr7S_Tx-PC-fa52r08v2_tdZSipeQVCWbYRckOd4lZTziRQqc2GTa6VjABnSjrVWKuUMoYYxkRtpeBggHDDl-j21GtyLCWDa1P2vc7fLSXtPFc7z9We5promxMdx_Qv-As9U21O</recordid><startdate>200711</startdate><enddate>200711</enddate><creator>Ikebukuro, Kazunori</creator><creator>Kumagai, Takenori</creator><creator>Motoki, Hiroaki</creator><creator>Osawa, Yuko</creator><creator>Matuo, Takafumi</creator><creator>Horiuchi, Michio</creator><creator>Sode, Koji</creator><general>Oxford University Press</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>200711</creationdate><title>Specific detection of PCR product from Legionella pneumophila strainPhiladelphia1 using zinc finger protein Sp2</title><author>Ikebukuro, Kazunori ; Kumagai, Takenori ; Motoki, Hiroaki ; Osawa, Yuko ; Matuo, Takafumi ; Horiuchi, Michio ; Sode, Koji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1043-e79d257851f93da1328e18ac52257d820e3298f96dd999cc0c2274d873ece03c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><toplevel>online_resources</toplevel><creatorcontrib>Ikebukuro, Kazunori</creatorcontrib><creatorcontrib>Kumagai, Takenori</creatorcontrib><creatorcontrib>Motoki, Hiroaki</creatorcontrib><creatorcontrib>Osawa, Yuko</creatorcontrib><creatorcontrib>Matuo, Takafumi</creatorcontrib><creatorcontrib>Horiuchi, Michio</creatorcontrib><creatorcontrib>Sode, Koji</creatorcontrib><collection>CrossRef</collection><jtitle>Nucleic Acids Symposium Series</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Ikebukuro, Kazunori</au><au>Kumagai, Takenori</au><au>Motoki, Hiroaki</au><au>Osawa, Yuko</au><au>Matuo, Takafumi</au><au>Horiuchi, Michio</au><au>Sode, Koji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specific detection of PCR product from Legionella pneumophila strainPhiladelphia1 using zinc finger protein Sp2</atitle><jtitle>Nucleic Acids Symposium Series</jtitle><date>2007-11</date><risdate>2007</risdate><volume>51</volume><issue>1</issue><spage>285</spage><epage>286</epage><pages>285-286</pages><issn>0261-3166</issn><eissn>1746-8272</eissn><abstract>We have developed a novel method to detect PCR products from pathogen genome DNA by Zinc finger protein that can bind to double strand DNA (dsDNA) in sequence specific manner. In this study, we tried to detect Legionella pneumophila strain Philadelphia 1 using Zinc finger protein. We found the specific target DNA sequence for zinc finger protein Sp2 in L. pneumophila strain Philadelphia 1 genome DNA. Specific PCR product was successfully amplified from L. pneumophila strain Philadelphia 1 genome DNA and we used Zinc finger protein Sp2 to detect it. We succeeded in detecting the PCR products from L. pneumophila strain Philadelphia 1 genome DNA with Sp2.</abstract><pub>Oxford University Press</pub><doi>10.1093/nass/nrm143</doi><tpages>2</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier ISSN: 0261-3166
ispartof Nucleic Acids Symposium Series, 2007-11, Vol.51 (1), p.285-286
issn 0261-3166
1746-8272
language eng
recordid cdi_crossref_primary_10_1093_nass_nrm143
source Oxford Journals Open Access Collection
title Specific detection of PCR product from Legionella pneumophila strainPhiladelphia1 using zinc finger protein Sp2
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-16T23%3A04%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-oup_TOX&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Specific%20detection%20of%20PCR%20product%20from%20Legionella%20pneumophila%20strainPhiladelphia1%20using%20zinc%20finger%20protein%20Sp2&rft.jtitle=Nucleic%20Acids%20Symposium%20Series&rft.au=Ikebukuro,%20Kazunori&rft.date=2007-11&rft.volume=51&rft.issue=1&rft.spage=285&rft.epage=286&rft.pages=285-286&rft.issn=0261-3166&rft.eissn=1746-8272&rft_id=info:doi/10.1093/nass/nrm143&rft_dat=%3Coup_TOX%3E10.1093/nass/nrm143%3C/oup_TOX%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_oup_id=10.1093/nass/nrm143&rfr_iscdi=true