Analysis of the interaction between human TLR3 ectodomain and nucleic acids

Analysis of the interaction between human TLR3 ectodomain and nucleic acids

Toll-like receptor 3 (TLR3) recognizes dsRNA of viral origin and polyriboinosine-polyribocytidylic acid (poly (I:C)). TLR3 mediates the activation of IRF-3 and NF-κB and thereby the secretion of type I interferons and inflammatory cytokines. However, the mechanism of this activation is poorly unders...

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Veröffentlicht in:Nucleic Acids Symposium Series 2006-11, Vol.50 (1), p.249-250
Hauptverfasser: Fukuda, Kotaro, Tsujita, Tadayuki, Matsumoto, Misako, Seya, Tsukasa, Sakiyama, Hiroshi, Nishikawa, Fumiko, Nishikawa, Satoshi, Hasegawa, Tsunemi
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container_end_page 250
container_issue 1
container_start_page 249
container_title Nucleic Acids Symposium Series
container_volume 50
creator Fukuda, Kotaro
Tsujita, Tadayuki
Matsumoto, Misako
Seya, Tsukasa
Sakiyama, Hiroshi
Nishikawa, Fumiko
Nishikawa, Satoshi
Hasegawa, Tsunemi
description Toll-like receptor 3 (TLR3) recognizes dsRNA of viral origin and polyriboinosine-polyribocytidylic acid (poly (I:C)). TLR3 mediates the activation of IRF-3 and NF-κB and thereby the secretion of type I interferons and inflammatory cytokines. However, the mechanism of this activation is poorly understood. To study the molecular recognition events and biochemical interactions between TLR3 and dsRNA, human TLR3 ectodomain (ECD) fused with a signal peptide at the N-terminus and 6 x His-tag at the C-terminus (TLR3-ECD) was obtained by the baculovirus expression system. To examine the various nucleic acids binding to TLR3-ECD in vitro, a filter binding assay was carried out at pH 4.2–7.6. Interaction of TLR3-ECD with various nucleic acids (particularly dsRNA, in vitro transcripts of tRNA and HCV NS3 aptamer) required an acidic pH.
doi_str_mv 10.1093/nass/nrl124
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title Analysis of the interaction between human TLR3 ectodomain and nucleic acids
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