Development of antibiotic marker-free creeping bentgrass resistance against herbicides
Herbicide-resistant creeping bentgrass plants （Agrostis stolonifera L.） without antibiotic-resistant markers were produced by Embryogenic Agrobacterium-mediated transtormation. callus tissues were infected with Agrobacterium tumefaciens EHA105, harboring： the bar and the CP4-EPSPS genes for bialapho...
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| Veröffentlicht in: | Acta biochimica et biophysica Sinica 2011, Vol.43 (1), p.13-18 |
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| container_title | Acta biochimica et biophysica Sinica |
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| creator | Lee, Ki-Won Kim, Ki-Yong Kim, Kyung-Hee Lee, Byung-Hyun Kim, Jin-Seog Lee, Sang-Hoon |
| description | Herbicide-resistant creeping bentgrass plants （Agrostis stolonifera L.） without antibiotic-resistant markers were produced by Embryogenic Agrobacterium-mediated transtormation. callus tissues were infected with Agrobacterium tumefaciens EHA105, harboring： the bar and the CP4-EPSPS genes for bialaphos and glyphosate resistance. Phosphinothricin-resistant calli and plants were selected. Soil-grown plants were obtained at 14-16 weeks after transformation. Genetic transformation of the selected, regenerated plants was validated by PCR. Southern blot analysis revealed that at least one copy of the transgene was integrated into the genome of the transgenic plants. Transgene expression was confirmed by Northern blot. CP4-EPSPS protein was detected by ELISA. Transgenic plants remained green and healthy when sprayed with Basta, containing 0.5% glufosinate ammonium or glyphosate. The optimized Agrobacteriummediated transformation method resulted in an average of 9.4% transgenic plants. The results of the present study suggest that the optimized marker-free technique could be used as an effective and reliable method for routine transformation, which may facilitate the development of varieties of new antibiotic-free grass species. |
| doi_str_mv | 10.1093/abbs/gmq106 |
| format | Article |
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Phosphinothricin-resistant calli and plants were selected. Soil-grown plants were obtained at 14-16 weeks after transformation. Genetic transformation of the selected, regenerated plants was validated by PCR. Southern blot analysis revealed that at least one copy of the transgene was integrated into the genome of the transgenic plants. Transgene expression was confirmed by Northern blot. CP4-EPSPS protein was detected by ELISA. Transgenic plants remained green and healthy when sprayed with Basta, containing 0.5% glufosinate ammonium or glyphosate. The optimized Agrobacteriummediated transformation method resulted in an average of 9.4% transgenic plants. The results of the present study suggest that the optimized marker-free technique could be used as an effective and reliable method for routine transformation, which may facilitate the development of varieties of new antibiotic-free grass species.</description><identifier>ISSN: 1672-9145</identifier><identifier>EISSN: 1745-7270</identifier><identifier>DOI: 10.1093/abbs/gmq106</identifier><identifier>PMID: 21173055</identifier><language>eng</language><publisher>China: Oxford University Press</publisher><subject>Agrobacterium tumefaciens - genetics ; Agrostis - drug effects ; Agrostis - genetics ; Aminobutyrates ; Drug Resistance, Microbial - genetics ; Glycine - analogs & derivatives ; Glyphosate ; Herbicide Resistance - genetics ; Herbicides - pharmacology ; Plants, Genetically Modified - genetics ; Transformation, Genetic ; Transgenes ; 农杆菌介导 ; 匍匐翦股颖 ; 抗性愈伤组织 ; 抗生素 ; 标记技术 ; 转基因植株 ; 除草剂Basta ; 除草剂抗性</subject><ispartof>Acta biochimica et biophysica Sinica, 2011, Vol.43 (1), p.13-18</ispartof><rights>The Author 2010. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c351t-7c067a1fe92d9644ea3b0d15fc9d4b5978f007fa779125abe8d54ad5105a21143</citedby><cites>FETCH-LOGICAL-c351t-7c067a1fe92d9644ea3b0d15fc9d4b5978f007fa779125abe8d54ad5105a21143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/90160X/90160X.jpg</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21173055$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Ki-Won</creatorcontrib><creatorcontrib>Kim, Ki-Yong</creatorcontrib><creatorcontrib>Kim, Kyung-Hee</creatorcontrib><creatorcontrib>Lee, Byung-Hyun</creatorcontrib><creatorcontrib>Kim, Jin-Seog</creatorcontrib><creatorcontrib>Lee, Sang-Hoon</creatorcontrib><title>Development of antibiotic marker-free creeping bentgrass resistance against herbicides</title><title>Acta biochimica et biophysica Sinica</title><addtitle>Acta Biochimica et Biophysica Sinica</addtitle><description>Herbicide-resistant creeping bentgrass plants （Agrostis stolonifera L.） without antibiotic-resistant markers were produced by Embryogenic Agrobacterium-mediated transtormation. callus tissues were infected with Agrobacterium tumefaciens EHA105, harboring： the bar and the CP4-EPSPS genes for bialaphos and glyphosate resistance. Phosphinothricin-resistant calli and plants were selected. Soil-grown plants were obtained at 14-16 weeks after transformation. Genetic transformation of the selected, regenerated plants was validated by PCR. Southern blot analysis revealed that at least one copy of the transgene was integrated into the genome of the transgenic plants. Transgene expression was confirmed by Northern blot. CP4-EPSPS protein was detected by ELISA. Transgenic plants remained green and healthy when sprayed with Basta, containing 0.5% glufosinate ammonium or glyphosate. The optimized Agrobacteriummediated transformation method resulted in an average of 9.4% transgenic plants. The results of the present study suggest that the optimized marker-free technique could be used as an effective and reliable method for routine transformation, which may facilitate the development of varieties of new antibiotic-free grass species.</description><subject>Agrobacterium tumefaciens - genetics</subject><subject>Agrostis - drug effects</subject><subject>Agrostis - genetics</subject><subject>Aminobutyrates</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Glycine - analogs & derivatives</subject><subject>Glyphosate</subject><subject>Herbicide Resistance - genetics</subject><subject>Herbicides - pharmacology</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Transformation, Genetic</subject><subject>Transgenes</subject><subject>农杆菌介导</subject><subject>匍匐翦股颖</subject><subject>抗性愈伤组织</subject><subject>抗生素</subject><subject>标记技术</subject><subject>转基因植株</subject><subject>除草剂Basta</subject><subject>除草剂抗性</subject><issn>1672-9145</issn><issn>1745-7270</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EoqUwsSOLgQWF2nEcNyMqn1IlFmCNzs45NW2T1E6R-Pe4CrCy3N3w6L27h5Bzzm44K8QUtA7TerPlLD8gY64ymahUscM45ypNCp7JETkJ4YMxkeecHZNRyrkSTMoxeb_DT1y33QabnraWQtM77dreGboBv0KfWI9ITSyda2qqI1d7CIF6DC700BikUINrQk-X6LUzrsJwSo4srAOe_fQJeXu4f50_JYuXx-f57SIxQvI-UYblCrjFIq2KPMsQhGYVl9YUVaZloWaWMWVBqYKnEjTOKplBJTmTEH_IxIRcD7nGtyF4tGXnXTz8q-Ss3Nsp93bKwU6kLwa62-kNVn_sr44IXA1Au-v-Sbr82btsm3obzZQazMq6NZZCpZLLVIhvk5J7Cg</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Lee, Ki-Won</creator><creator>Kim, Ki-Yong</creator><creator>Kim, Kyung-Hee</creator><creator>Lee, Byung-Hyun</creator><creator>Kim, Jin-Seog</creator><creator>Lee, Sang-Hoon</creator><general>Oxford University Press</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W95</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2011</creationdate><title>Development of antibiotic marker-free creeping bentgrass resistance against herbicides</title><author>Lee, Ki-Won ; Kim, Ki-Yong ; Kim, Kyung-Hee ; Lee, Byung-Hyun ; Kim, Jin-Seog ; Lee, Sang-Hoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-7c067a1fe92d9644ea3b0d15fc9d4b5978f007fa779125abe8d54ad5105a21143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Agrobacterium tumefaciens - genetics</topic><topic>Agrostis - drug effects</topic><topic>Agrostis - genetics</topic><topic>Aminobutyrates</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Glycine - analogs & derivatives</topic><topic>Glyphosate</topic><topic>Herbicide Resistance - genetics</topic><topic>Herbicides - pharmacology</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Transformation, Genetic</topic><topic>Transgenes</topic><topic>农杆菌介导</topic><topic>匍匐翦股颖</topic><topic>抗性愈伤组织</topic><topic>抗生素</topic><topic>标记技术</topic><topic>转基因植株</topic><topic>除草剂Basta</topic><topic>除草剂抗性</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Ki-Won</creatorcontrib><creatorcontrib>Kim, Ki-Yong</creatorcontrib><creatorcontrib>Kim, Kyung-Hee</creatorcontrib><creatorcontrib>Lee, Byung-Hyun</creatorcontrib><creatorcontrib>Kim, Jin-Seog</creatorcontrib><creatorcontrib>Lee, Sang-Hoon</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-农业科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Acta biochimica et biophysica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Ki-Won</au><au>Kim, Ki-Yong</au><au>Kim, Kyung-Hee</au><au>Lee, Byung-Hyun</au><au>Kim, Jin-Seog</au><au>Lee, Sang-Hoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of antibiotic marker-free creeping bentgrass resistance against herbicides</atitle><jtitle>Acta biochimica et biophysica Sinica</jtitle><addtitle>Acta Biochimica et Biophysica Sinica</addtitle><date>2011</date><risdate>2011</risdate><volume>43</volume><issue>1</issue><spage>13</spage><epage>18</epage><pages>13-18</pages><issn>1672-9145</issn><eissn>1745-7270</eissn><abstract>Herbicide-resistant creeping bentgrass plants （Agrostis stolonifera L.） without antibiotic-resistant markers were produced by Embryogenic Agrobacterium-mediated transtormation. callus tissues were infected with Agrobacterium tumefaciens EHA105, harboring： the bar and the CP4-EPSPS genes for bialaphos and glyphosate resistance. Phosphinothricin-resistant calli and plants were selected. Soil-grown plants were obtained at 14-16 weeks after transformation. Genetic transformation of the selected, regenerated plants was validated by PCR. Southern blot analysis revealed that at least one copy of the transgene was integrated into the genome of the transgenic plants. Transgene expression was confirmed by Northern blot. CP4-EPSPS protein was detected by ELISA. Transgenic plants remained green and healthy when sprayed with Basta, containing 0.5% glufosinate ammonium or glyphosate. The optimized Agrobacteriummediated transformation method resulted in an average of 9.4% transgenic plants. The results of the present study suggest that the optimized marker-free technique could be used as an effective and reliable method for routine transformation, which may facilitate the development of varieties of new antibiotic-free grass species.</abstract><cop>China</cop><pub>Oxford University Press</pub><pmid>21173055</pmid><doi>10.1093/abbs/gmq106</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Agrobacterium tumefaciens - genetics Agrostis - drug effects Agrostis - genetics Aminobutyrates Drug Resistance, Microbial - genetics Glycine - analogs & derivatives Glyphosate Herbicide Resistance - genetics Herbicides - pharmacology Plants, Genetically Modified - genetics Transformation, Genetic Transgenes 农杆菌介导 匍匐翦股颖 抗性愈伤组织 抗生素 标记技术 转基因植株 除草剂Basta 除草剂抗性 |
| title | Development of antibiotic marker-free creeping bentgrass resistance against herbicides |
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