Incompatibility Mechanism Between Radix Paeoniae Alba and Veratrum nigrum Focusing on Estrogen-Estrogen Receptor Pathway in Immature/Ovariectomized Mice
Radix paeoniae alba (RPA) and Veratrum nigrum (VN) L . belong to the 18 incompatible medicaments and have been prohibited for thousands of years in China. Previous studies focused on the chemical constituents that induced the toxicological response of the two agents, but this study offers preliminar...
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| Veröffentlicht in: | Rejuvenation research 2019-12, Vol.22 (6), p.465-477 |
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| creator | Xu, Ying Li, Xin Chen, Ting Qu, Ya-Kun Zheng, Hong-Xia Zhang, Zi-Jia Zhao, Yuan Lin, Na |
| description | Radix paeoniae alba
(RPA) and
Veratrum nigrum
(VN)
L
. belong to the 18 incompatible medicaments and have been prohibited for thousands of years in China. Previous studies focused on the chemical constituents that induced the toxicological response of the two agents, but this study offers preliminary insight into the pharmacodynamics and mechanism on estrogenic activity, which is responsible for their incompatibility. We undertook a characterization of the interaction on estrogenic activity of RPA and VN using
in vivo
models of immature and ovariectomized (OVX) mice and
in vitro
studies focused on estrogen receptor (ER) pathway for further mechanism. VN disturbed the estrogenic efficacy of RPA in promoting development of uterus and vagina in immature mice, and reversing the atrophy of reproductive tissues in OVX mice by decreasing the increase of serum estrogen level and upregulation of ER expression in reproductive tissues by treatment with RPA. Besides, VN antagonized the estrogenic efficacy of RPA in stimulating the binding with ERα and ERβ, increasing ERα/β-estrogen response element (ERE) luciferase reporter gene expression and promoting MCF-7 cell viability. This study provided evidence that VN antagonized the estrogenic efficacy of RPA by decreasing the up-regulations of estrogen biosynthesis in circulation and ERs in target tissues caused by RPA, and through ER-ERE-dependent pathway. |
| doi_str_mv | 10.1089/rej.2017.2026 |
| format | Article |
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(RPA) and
Veratrum nigrum
(VN)
L
. belong to the 18 incompatible medicaments and have been prohibited for thousands of years in China. Previous studies focused on the chemical constituents that induced the toxicological response of the two agents, but this study offers preliminary insight into the pharmacodynamics and mechanism on estrogenic activity, which is responsible for their incompatibility. We undertook a characterization of the interaction on estrogenic activity of RPA and VN using
in vivo
models of immature and ovariectomized (OVX) mice and
in vitro
studies focused on estrogen receptor (ER) pathway for further mechanism. VN disturbed the estrogenic efficacy of RPA in promoting development of uterus and vagina in immature mice, and reversing the atrophy of reproductive tissues in OVX mice by decreasing the increase of serum estrogen level and upregulation of ER expression in reproductive tissues by treatment with RPA. Besides, VN antagonized the estrogenic efficacy of RPA in stimulating the binding with ERα and ERβ, increasing ERα/β-estrogen response element (ERE) luciferase reporter gene expression and promoting MCF-7 cell viability. This study provided evidence that VN antagonized the estrogenic efficacy of RPA by decreasing the up-regulations of estrogen biosynthesis in circulation and ERs in target tissues caused by RPA, and through ER-ERE-dependent pathway.</description><identifier>ISSN: 1549-1684</identifier><identifier>EISSN: 1557-8577</identifier><identifier>DOI: 10.1089/rej.2017.2026</identifier><identifier>PMID: 30672382</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc., publishers</publisher><subject>Animals ; Estrogens - metabolism ; Female ; Humans ; MCF-7 Cells ; Mice ; Original Articles ; Ovariectomy ; Ovary - drug effects ; Ovary - metabolism ; Ovary - surgery ; Paeonia - chemistry ; Plant Extracts - pharmacology ; Receptors, Estrogen - metabolism ; Uterus - drug effects ; Uterus - metabolism ; Uterus - surgery ; Vagina - drug effects ; Vagina - metabolism ; Vagina - surgery ; Veratrum - chemistry</subject><ispartof>Rejuvenation research, 2019-12, Vol.22 (6), p.465-477</ispartof><rights>2019, Mary Ann Liebert, Inc., publishers</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-cc8f9d18393112c3d3f1249caf5c7495d1fbe39df6c18bf703e1297d9d7bcc1e3</citedby><cites>FETCH-LOGICAL-c337t-cc8f9d18393112c3d3f1249caf5c7495d1fbe39df6c18bf703e1297d9d7bcc1e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30672382$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Ying</creatorcontrib><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Chen, Ting</creatorcontrib><creatorcontrib>Qu, Ya-Kun</creatorcontrib><creatorcontrib>Zheng, Hong-Xia</creatorcontrib><creatorcontrib>Zhang, Zi-Jia</creatorcontrib><creatorcontrib>Zhao, Yuan</creatorcontrib><creatorcontrib>Lin, Na</creatorcontrib><title>Incompatibility Mechanism Between Radix Paeoniae Alba and Veratrum nigrum Focusing on Estrogen-Estrogen Receptor Pathway in Immature/Ovariectomized Mice</title><title>Rejuvenation research</title><addtitle>Rejuvenation Res</addtitle><description>Radix paeoniae alba
(RPA) and
Veratrum nigrum
(VN)
L
. belong to the 18 incompatible medicaments and have been prohibited for thousands of years in China. Previous studies focused on the chemical constituents that induced the toxicological response of the two agents, but this study offers preliminary insight into the pharmacodynamics and mechanism on estrogenic activity, which is responsible for their incompatibility. We undertook a characterization of the interaction on estrogenic activity of RPA and VN using
in vivo
models of immature and ovariectomized (OVX) mice and
in vitro
studies focused on estrogen receptor (ER) pathway for further mechanism. VN disturbed the estrogenic efficacy of RPA in promoting development of uterus and vagina in immature mice, and reversing the atrophy of reproductive tissues in OVX mice by decreasing the increase of serum estrogen level and upregulation of ER expression in reproductive tissues by treatment with RPA. Besides, VN antagonized the estrogenic efficacy of RPA in stimulating the binding with ERα and ERβ, increasing ERα/β-estrogen response element (ERE) luciferase reporter gene expression and promoting MCF-7 cell viability. This study provided evidence that VN antagonized the estrogenic efficacy of RPA by decreasing the up-regulations of estrogen biosynthesis in circulation and ERs in target tissues caused by RPA, and through ER-ERE-dependent pathway.</description><subject>Animals</subject><subject>Estrogens - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>MCF-7 Cells</subject><subject>Mice</subject><subject>Original Articles</subject><subject>Ovariectomy</subject><subject>Ovary - drug effects</subject><subject>Ovary - metabolism</subject><subject>Ovary - surgery</subject><subject>Paeonia - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Receptors, Estrogen - metabolism</subject><subject>Uterus - drug effects</subject><subject>Uterus - metabolism</subject><subject>Uterus - surgery</subject><subject>Vagina - drug effects</subject><subject>Vagina - metabolism</subject><subject>Vagina - surgery</subject><subject>Veratrum - chemistry</subject><issn>1549-1684</issn><issn>1557-8577</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFPwjAYhhujEUSPXk3_wGBdt3U9IgElgWCIel269huUbN3SFRF_iT_XLYhXL9_75suT9_AgdE_8IfETPrKwGwY-Ye0J4gvUJ1HEvCRi7LLrIfdInIQ9dNM0O98PGI-ia9SjfswCmgR99D03sipr4XSmC-2OeAlyK4xuSvwI7gBg8Foo_YlfBFRGC8DjIhNYGIXfwQpn9yU2etPFrJL7RpsNrgyeNs5WGzDeueA1SKhdZdshtz2II9YGz8tSuL2F0epDWA3SVaX-AoWXWsItuspF0cDdbw7Q22z6Onn2Fqun-WS88CSlzHlSJjlXJKGcEhJIqmhOgpBLkUeShTxSJM-AcpXHkiRZznwKJOBMccUyKQnQAfJOu9JWTWMhT2urS2GPKfHTznDaGk47w2lnuOUfTny9z0pQf_RZaQvQE9C9hTGFhgys-2f2B3cOjG8</recordid><startdate>20191201</startdate><enddate>20191201</enddate><creator>Xu, Ying</creator><creator>Li, Xin</creator><creator>Chen, Ting</creator><creator>Qu, Ya-Kun</creator><creator>Zheng, Hong-Xia</creator><creator>Zhang, Zi-Jia</creator><creator>Zhao, Yuan</creator><creator>Lin, Na</creator><general>Mary Ann Liebert, Inc., publishers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20191201</creationdate><title>Incompatibility Mechanism Between Radix Paeoniae Alba and Veratrum nigrum Focusing on Estrogen-Estrogen Receptor Pathway in Immature/Ovariectomized Mice</title><author>Xu, Ying ; Li, Xin ; Chen, Ting ; Qu, Ya-Kun ; Zheng, Hong-Xia ; Zhang, Zi-Jia ; Zhao, Yuan ; Lin, Na</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-cc8f9d18393112c3d3f1249caf5c7495d1fbe39df6c18bf703e1297d9d7bcc1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Estrogens - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>MCF-7 Cells</topic><topic>Mice</topic><topic>Original Articles</topic><topic>Ovariectomy</topic><topic>Ovary - drug effects</topic><topic>Ovary - metabolism</topic><topic>Ovary - surgery</topic><topic>Paeonia - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Receptors, Estrogen - metabolism</topic><topic>Uterus - drug effects</topic><topic>Uterus - metabolism</topic><topic>Uterus - surgery</topic><topic>Vagina - drug effects</topic><topic>Vagina - metabolism</topic><topic>Vagina - surgery</topic><topic>Veratrum - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Ying</creatorcontrib><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Chen, Ting</creatorcontrib><creatorcontrib>Qu, Ya-Kun</creatorcontrib><creatorcontrib>Zheng, Hong-Xia</creatorcontrib><creatorcontrib>Zhang, Zi-Jia</creatorcontrib><creatorcontrib>Zhao, Yuan</creatorcontrib><creatorcontrib>Lin, Na</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Rejuvenation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Ying</au><au>Li, Xin</au><au>Chen, Ting</au><au>Qu, Ya-Kun</au><au>Zheng, Hong-Xia</au><au>Zhang, Zi-Jia</au><au>Zhao, Yuan</au><au>Lin, Na</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Incompatibility Mechanism Between Radix Paeoniae Alba and Veratrum nigrum Focusing on Estrogen-Estrogen Receptor Pathway in Immature/Ovariectomized Mice</atitle><jtitle>Rejuvenation research</jtitle><addtitle>Rejuvenation Res</addtitle><date>2019-12-01</date><risdate>2019</risdate><volume>22</volume><issue>6</issue><spage>465</spage><epage>477</epage><pages>465-477</pages><issn>1549-1684</issn><eissn>1557-8577</eissn><abstract>Radix paeoniae alba
(RPA) and
Veratrum nigrum
(VN)
L
. belong to the 18 incompatible medicaments and have been prohibited for thousands of years in China. Previous studies focused on the chemical constituents that induced the toxicological response of the two agents, but this study offers preliminary insight into the pharmacodynamics and mechanism on estrogenic activity, which is responsible for their incompatibility. We undertook a characterization of the interaction on estrogenic activity of RPA and VN using
in vivo
models of immature and ovariectomized (OVX) mice and
in vitro
studies focused on estrogen receptor (ER) pathway for further mechanism. VN disturbed the estrogenic efficacy of RPA in promoting development of uterus and vagina in immature mice, and reversing the atrophy of reproductive tissues in OVX mice by decreasing the increase of serum estrogen level and upregulation of ER expression in reproductive tissues by treatment with RPA. Besides, VN antagonized the estrogenic efficacy of RPA in stimulating the binding with ERα and ERβ, increasing ERα/β-estrogen response element (ERE) luciferase reporter gene expression and promoting MCF-7 cell viability. This study provided evidence that VN antagonized the estrogenic efficacy of RPA by decreasing the up-regulations of estrogen biosynthesis in circulation and ERs in target tissues caused by RPA, and through ER-ERE-dependent pathway.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc., publishers</pub><pmid>30672382</pmid><doi>10.1089/rej.2017.2026</doi><tpages>13</tpages></addata></record> |
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| ispartof | Rejuvenation research, 2019-12, Vol.22 (6), p.465-477 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Estrogens - metabolism Female Humans MCF-7 Cells Mice Original Articles Ovariectomy Ovary - drug effects Ovary - metabolism Ovary - surgery Paeonia - chemistry Plant Extracts - pharmacology Receptors, Estrogen - metabolism Uterus - drug effects Uterus - metabolism Uterus - surgery Vagina - drug effects Vagina - metabolism Vagina - surgery Veratrum - chemistry |
| title | Incompatibility Mechanism Between Radix Paeoniae Alba and Veratrum nigrum Focusing on Estrogen-Estrogen Receptor Pathway in Immature/Ovariectomized Mice |
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