Synergistic Action of Starch and Honey Against Pseudomonas aeruginosa in Correlation with Diastase Number
To evaluate the synergistic action of starch on the antibacterial activity of honey against Pseudomonas aeruginosa , a comparative method of adding honey with and without starch to culture media was used. P. aeruginosa (ATCC 27853) was used to determine the minimum inhibitory concentration (MIC) of...
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| Veröffentlicht in: | The journal of alternative and complementary medicine (New York, N.Y.) N.Y.), 2008-03, Vol.14 (2), p.181-184 |
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| container_title | The journal of alternative and complementary medicine (New York, N.Y.) |
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| creator | Boukraa, Laïd Benbarek, Hama Aissat, Saâd |
| description | To evaluate the synergistic action of starch on the antibacterial activity of honey against
Pseudomonas aeruginosa
, a comparative method of adding honey with and without starch to culture media was used.
P. aeruginosa
(ATCC 27853) was used to determine the minimum inhibitory concentration (MIC) of five varieties of honey. In a second step, lower concentrations of honey than the MIC were incubated with a set of concentrations of starch and then added to media to determine the minimum synergistic inhibitory concentration. The MIC for the five varieties of honey without starch against
P. aeruginosa
ranged between 15% and 26% (vol/vol). When starch was incubated with honey and then added to media, a significant MIC drop has been noticed with each variety and it ranged between 30.7% and 46.6%. No significant correlation has been established between the MIC drop and the diastase number. |
| doi_str_mv | 10.1089/acm.2007.0589 |
| format | Article |
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Pseudomonas aeruginosa
, a comparative method of adding honey with and without starch to culture media was used.
P. aeruginosa
(ATCC 27853) was used to determine the minimum inhibitory concentration (MIC) of five varieties of honey. In a second step, lower concentrations of honey than the MIC were incubated with a set of concentrations of starch and then added to media to determine the minimum synergistic inhibitory concentration. The MIC for the five varieties of honey without starch against
P. aeruginosa
ranged between 15% and 26% (vol/vol). When starch was incubated with honey and then added to media, a significant MIC drop has been noticed with each variety and it ranged between 30.7% and 46.6%. No significant correlation has been established between the MIC drop and the diastase number.</description><identifier>ISSN: 1075-5535</identifier><identifier>EISSN: 1557-7708</identifier><identifier>DOI: 10.1089/acm.2007.0589</identifier><identifier>PMID: 18315514</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Amylases - drug effects ; Anti-Bacterial Agents - pharmacology ; Colony Count, Microbial ; Dose-Response Relationship, Drug ; Drug Synergism ; Honey ; Microbial Sensitivity Tests ; ORIGINAL PAPERS ; Pseudomonas aeruginosa - drug effects ; Pseudomonas aeruginosa - enzymology ; Pseudomonas aeruginosa - growth & development ; Starch - administration & dosage</subject><ispartof>The journal of alternative and complementary medicine (New York, N.Y.), 2008-03, Vol.14 (2), p.181-184</ispartof><rights>2008 Mary Ann Liebert, Inc.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c327t-810dc55f40f7bd029f4b88c6a016ca3a4c98497d2308aee96a19ec758765fd5b3</citedby><cites>FETCH-LOGICAL-c327t-810dc55f40f7bd029f4b88c6a016ca3a4c98497d2308aee96a19ec758765fd5b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18315514$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boukraa, Laïd</creatorcontrib><creatorcontrib>Benbarek, Hama</creatorcontrib><creatorcontrib>Aissat, Saâd</creatorcontrib><title>Synergistic Action of Starch and Honey Against Pseudomonas aeruginosa in Correlation with Diastase Number</title><title>The journal of alternative and complementary medicine (New York, N.Y.)</title><addtitle>J Altern Complement Med</addtitle><description>To evaluate the synergistic action of starch on the antibacterial activity of honey against
Pseudomonas aeruginosa
, a comparative method of adding honey with and without starch to culture media was used.
P. aeruginosa
(ATCC 27853) was used to determine the minimum inhibitory concentration (MIC) of five varieties of honey. In a second step, lower concentrations of honey than the MIC were incubated with a set of concentrations of starch and then added to media to determine the minimum synergistic inhibitory concentration. The MIC for the five varieties of honey without starch against
P. aeruginosa
ranged between 15% and 26% (vol/vol). When starch was incubated with honey and then added to media, a significant MIC drop has been noticed with each variety and it ranged between 30.7% and 46.6%. No significant correlation has been established between the MIC drop and the diastase number.</description><subject>Amylases - drug effects</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Colony Count, Microbial</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Synergism</subject><subject>Honey</subject><subject>Microbial Sensitivity Tests</subject><subject>ORIGINAL PAPERS</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Pseudomonas aeruginosa - enzymology</subject><subject>Pseudomonas aeruginosa - growth & development</subject><subject>Starch - administration & dosage</subject><issn>1075-5535</issn><issn>1557-7708</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM9LwzAUgIMobk6PXiV36UzapkmOY_6YMFSYnstrmm6RNRlJiuy_t3VD8fQej48P3ofQNSVTSoS8A9VOU0L4lDAhT9CYMsYTzok47XfCWcJYxkboIoRPQkiey_QcjajIeo7mY2RWe6v92oRoFJ6paJzFrsGrCF5tMNgaL5zVezxbg7Eh4regu9q1zkLAoH23NtYFwMbiufNeb-HH8GXiBt8bCBGCxi9dW2l_ic4a2AZ9dZwT9PH48D5fJMvXp-f5bJmoLOUxEZTUirEmJw2vapLKJq-EUAUQWijIIFdS5JLXaUYEaC0LoFIrzgQvWFOzKpug5OBV3oXgdVPuvGnB70tKyiFZ2Scrh2TlkKznbw78rqtaXf_Rx0Y9cHsAhjNYuzW6fyf-gv913y3-eAY</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Boukraa, Laïd</creator><creator>Benbarek, Hama</creator><creator>Aissat, Saâd</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20080301</creationdate><title>Synergistic Action of Starch and Honey Against Pseudomonas aeruginosa in Correlation with Diastase Number</title><author>Boukraa, Laïd ; Benbarek, Hama ; Aissat, Saâd</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c327t-810dc55f40f7bd029f4b88c6a016ca3a4c98497d2308aee96a19ec758765fd5b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Amylases - drug effects</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Colony Count, Microbial</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Synergism</topic><topic>Honey</topic><topic>Microbial Sensitivity Tests</topic><topic>ORIGINAL PAPERS</topic><topic>Pseudomonas aeruginosa - drug effects</topic><topic>Pseudomonas aeruginosa - enzymology</topic><topic>Pseudomonas aeruginosa - growth & development</topic><topic>Starch - administration & dosage</topic><toplevel>online_resources</toplevel><creatorcontrib>Boukraa, Laïd</creatorcontrib><creatorcontrib>Benbarek, Hama</creatorcontrib><creatorcontrib>Aissat, Saâd</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>The journal of alternative and complementary medicine (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boukraa, Laïd</au><au>Benbarek, Hama</au><au>Aissat, Saâd</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synergistic Action of Starch and Honey Against Pseudomonas aeruginosa in Correlation with Diastase Number</atitle><jtitle>The journal of alternative and complementary medicine (New York, N.Y.)</jtitle><addtitle>J Altern Complement Med</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>14</volume><issue>2</issue><spage>181</spage><epage>184</epage><pages>181-184</pages><issn>1075-5535</issn><eissn>1557-7708</eissn><abstract>To evaluate the synergistic action of starch on the antibacterial activity of honey against
Pseudomonas aeruginosa
, a comparative method of adding honey with and without starch to culture media was used.
P. aeruginosa
(ATCC 27853) was used to determine the minimum inhibitory concentration (MIC) of five varieties of honey. In a second step, lower concentrations of honey than the MIC were incubated with a set of concentrations of starch and then added to media to determine the minimum synergistic inhibitory concentration. The MIC for the five varieties of honey without starch against
P. aeruginosa
ranged between 15% and 26% (vol/vol). When starch was incubated with honey and then added to media, a significant MIC drop has been noticed with each variety and it ranged between 30.7% and 46.6%. No significant correlation has been established between the MIC drop and the diastase number.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>18315514</pmid><doi>10.1089/acm.2007.0589</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 1075-5535 |
| ispartof | The journal of alternative and complementary medicine (New York, N.Y.), 2008-03, Vol.14 (2), p.181-184 |
| issn | 1075-5535 1557-7708 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Amylases - drug effects Anti-Bacterial Agents - pharmacology Colony Count, Microbial Dose-Response Relationship, Drug Drug Synergism Honey Microbial Sensitivity Tests ORIGINAL PAPERS Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - enzymology Pseudomonas aeruginosa - growth & development Starch - administration & dosage |
| title | Synergistic Action of Starch and Honey Against Pseudomonas aeruginosa in Correlation with Diastase Number |
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