Attachment of microstructures to single bacteria by two-photon patterning of a protein based hydrogel
We investigate the fabrication and attachment of microstructures to bacteria by two photon laser lithography in single cell experiments. Bacteria are embedded in a protein based hydrogel precursor and crosslinking is used to attach microstructures, resulting in a motility change or complete immobili...
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| Veröffentlicht in: | Biomedical physics & engineering express 2018-03, Vol.4 (3), p.35004 |
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| container_title | Biomedical physics & engineering express |
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| creator | Hasselmann, Nils Frederik Horn, Wolfgang |
| description | We investigate the fabrication and attachment of microstructures to bacteria by two photon laser lithography in single cell experiments. Bacteria are embedded in a protein based hydrogel precursor and crosslinking is used to attach microstructures, resulting in a motility change or complete immobilization of a selected bacterium. The processing parameters of the compound are characterized and the viability of the bacteria exposed to the precursor solution is evaluated by cell leakage and motility assays pre-and post-fabrication. |
| doi_str_mv | 10.1088/2057-1976/aaafb7 |
| format | Article |
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Bacteria are embedded in a protein based hydrogel precursor and crosslinking is used to attach microstructures, resulting in a motility change or complete immobilization of a selected bacterium. 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Phys. Eng. Express</addtitle><description>We investigate the fabrication and attachment of microstructures to bacteria by two photon laser lithography in single cell experiments. Bacteria are embedded in a protein based hydrogel precursor and crosslinking is used to attach microstructures, resulting in a motility change or complete immobilization of a selected bacterium. The processing parameters of the compound are characterized and the viability of the bacteria exposed to the precursor solution is evaluated by cell leakage and motility assays pre-and post-fabrication.</description><subject>3D bioprinting</subject><subject>bacteria immobilization</subject><subject>bacteria powered microrobotics</subject><subject>BSA</subject><subject>FMN</subject><subject>microbiorobotics</subject><subject>two photon polymerization</subject><issn>2057-1976</issn><issn>2057-1976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kM1LAzEQxYMoWLR3j7l5cW0-utnNsRS_oOCl9zDJTtot7WZJUrT_vbtUxIN4muHxe8ObR8gdZ4-c1fVMsLIquK7UDAC8rS7I5Ee6_LVfk2lKO8YYV0IpXU4ILnIGtz1gl2nw9NC6GFKOR5ePERPNgaa22-yRWnAZYwvUnmj-CEW_DTl0tIc8yN3AjHagfQwZ227AEzZ0e2pi2OD-llx52Cecfs8bsn5-Wi9fi9X7y9tysSqc5CIXKDyABlY57wU2FrS3pS61tlb7IbWuVc1BVFo5x70UtXau4co3DiT3KG8IO58dn0gRvelje4B4MpyZsSgzNmHGJsy5qMHycLa0oTe7cIzdkO8__P4P3Pb4aeZGGiZLxuamb7z8AvUxe1w</recordid><startdate>20180306</startdate><enddate>20180306</enddate><creator>Hasselmann, Nils Frederik</creator><creator>Horn, Wolfgang</creator><general>IOP Publishing</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-5923-3007</orcidid></search><sort><creationdate>20180306</creationdate><title>Attachment of microstructures to single bacteria by two-photon patterning of a protein based hydrogel</title><author>Hasselmann, Nils Frederik ; Horn, Wolfgang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c312t-e2faa9a07cff2edba9fb59599bb9f00098681a2796cc1f3289ccd16fdca31fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>3D bioprinting</topic><topic>bacteria immobilization</topic><topic>bacteria powered microrobotics</topic><topic>BSA</topic><topic>FMN</topic><topic>microbiorobotics</topic><topic>two photon polymerization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hasselmann, Nils Frederik</creatorcontrib><creatorcontrib>Horn, Wolfgang</creatorcontrib><collection>CrossRef</collection><jtitle>Biomedical physics & engineering express</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hasselmann, Nils Frederik</au><au>Horn, Wolfgang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Attachment of microstructures to single bacteria by two-photon patterning of a protein based hydrogel</atitle><jtitle>Biomedical physics & engineering express</jtitle><stitle>BPEX</stitle><addtitle>Biomed. Phys. Eng. Express</addtitle><date>2018-03-06</date><risdate>2018</risdate><volume>4</volume><issue>3</issue><spage>35004</spage><pages>35004-</pages><issn>2057-1976</issn><eissn>2057-1976</eissn><abstract>We investigate the fabrication and attachment of microstructures to bacteria by two photon laser lithography in single cell experiments. Bacteria are embedded in a protein based hydrogel precursor and crosslinking is used to attach microstructures, resulting in a motility change or complete immobilization of a selected bacterium. The processing parameters of the compound are characterized and the viability of the bacteria exposed to the precursor solution is evaluated by cell leakage and motility assays pre-and post-fabrication.</abstract><pub>IOP Publishing</pub><doi>10.1088/2057-1976/aaafb7</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-5923-3007</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2057-1976 |
| ispartof | Biomedical physics & engineering express, 2018-03, Vol.4 (3), p.35004 |
| issn | 2057-1976 2057-1976 |
| language | eng |
| recordid | cdi_crossref_primary_10_1088_2057_1976_aaafb7 |
| source | IOP Publishing Journals; Institute of Physics (IOP) Journals - HEAL-Link |
| subjects | 3D bioprinting bacteria immobilization bacteria powered microrobotics BSA FMN microbiorobotics two photon polymerization |
| title | Attachment of microstructures to single bacteria by two-photon patterning of a protein based hydrogel |
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