Maximum entropy analysis of polarized fluorescence decay of (E)GFP in aqueous solution

The maximum entropy method (MEM) was used for the analysis of polarized fluorescence decays of enhanced green fluorescent protein (EGFP) in buffered water/glycerol mixtures, obtained with time-correlated single-photon counting (Visser et al 2016 Methods Appl. Fluoresc. 4 035002). To this end, we use...

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Veröffentlicht in:	Methods and applications in fluorescence 2018-01, Vol.6 (1), p.014001-014001
Hauptverfasser:	Novikov, Eugene G, Skakun, Victor V, Borst, Jan Willem, Visser, Antonie J W G
Format:	Artikel
Sprache:	eng
Schlagworte:	(enhanced) green fluorescent protein Maximum entropy method Rate spectrum Rotational correlation time Rotational diffusion Time-resolved fluorescence Time-resolved fluorescence anisotropy
Online-Zugang:	Volltext
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