Biopolymeric corneal lenticules by digital light processing based bioprinting: a dynamic substitute for corneal transplant
Digital light processing (DLP) technology has gained significant attention for its ability to construct intricate structures for various applications in tissue modeling and regeneration. In this study, we aimed to design corneal lenticules using DLP bioprinting technology, utilizing dual network bio...
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| Veröffentlicht in: | Biomedical materials (Bristol) 2024-05, Vol.19 (3), p.35017 |
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| creator | Bhutani, Utkarsh Dey, Namit Chowdhury, Suvro Kanti Waghmare, Neha Mahapatra, Rita Das Selvakumar, Kamalnath Chandru, Arun Bhowmick, Tuhin Agrawal, Parinita |
| description | Digital light processing (DLP) technology has gained significant attention for its ability to construct intricate structures for various applications in tissue modeling and regeneration. In this study, we aimed to design corneal lenticules using DLP bioprinting technology, utilizing dual network bioinks to mimic the characteristics of the human cornea. The bioink was prepared using methacrylated hyaluronic acid and methacrylated gelatin, where ruthenium salt and sodium persulfate were included for mediating photo-crosslinking while tartrazine was used as a photoabsorber. The bioprinted lenticules were optically transparent (85.45% ± 0.14%), exhibited adhesive strength (58.67 ± 17.5 kPa), and compressive modulus (535.42 ± 29.05 kPa) sufficient for supporting corneal tissue integration and regeneration. Puncture resistance tests and drag force analysis further confirmed the excellent mechanical performance of the lenticules enabling their application as potential corneal implants. Additionally, the lenticules demonstrated outstanding support for re-epithelialization and stromal regeneration when assessed with human corneal stromal cells. We generated implant ready corneal lenticules while optimizing bioink and bioprinting parameters, providing valuable solution for individuals suffering from various corneal defects and waiting for corneal transplants. |
| doi_str_mv | 10.1088/1748-605X/ad3312 |
| format | Article |
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In this study, we aimed to design corneal lenticules using DLP bioprinting technology, utilizing dual network bioinks to mimic the characteristics of the human cornea. The bioink was prepared using methacrylated hyaluronic acid and methacrylated gelatin, where ruthenium salt and sodium persulfate were included for mediating photo-crosslinking while tartrazine was used as a photoabsorber. The bioprinted lenticules were optically transparent (85.45% ± 0.14%), exhibited adhesive strength (58.67 ± 17.5 kPa), and compressive modulus (535.42 ± 29.05 kPa) sufficient for supporting corneal tissue integration and regeneration. Puncture resistance tests and drag force analysis further confirmed the excellent mechanical performance of the lenticules enabling their application as potential corneal implants. Additionally, the lenticules demonstrated outstanding support for re-epithelialization and stromal regeneration when assessed with human corneal stromal cells. 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Mater</addtitle><description>Digital light processing (DLP) technology has gained significant attention for its ability to construct intricate structures for various applications in tissue modeling and regeneration. In this study, we aimed to design corneal lenticules using DLP bioprinting technology, utilizing dual network bioinks to mimic the characteristics of the human cornea. The bioink was prepared using methacrylated hyaluronic acid and methacrylated gelatin, where ruthenium salt and sodium persulfate were included for mediating photo-crosslinking while tartrazine was used as a photoabsorber. The bioprinted lenticules were optically transparent (85.45% ± 0.14%), exhibited adhesive strength (58.67 ± 17.5 kPa), and compressive modulus (535.42 ± 29.05 kPa) sufficient for supporting corneal tissue integration and regeneration. Puncture resistance tests and drag force analysis further confirmed the excellent mechanical performance of the lenticules enabling their application as potential corneal implants. Additionally, the lenticules demonstrated outstanding support for re-epithelialization and stromal regeneration when assessed with human corneal stromal cells. We generated implant ready corneal lenticules while optimizing bioink and bioprinting parameters, providing valuable solution for individuals suffering from various corneal defects and waiting for corneal transplants.</description><subject>bioink</subject><subject>Bioprinting</subject><subject>Cornea</subject><subject>cornea lenticule</subject><subject>Corneal Transplantation</subject><subject>digital light processing</subject><subject>gelatin</subject><subject>Humans</subject><subject>hyaluronic acid</subject><subject>Hydrogels</subject><subject>Printing, Three-Dimensional</subject><subject>Tissue Engineering</subject><subject>Tissue Scaffolds - chemistry</subject><issn>1748-6041</issn><issn>1748-605X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDtPwzAURi0EoqWwMyFvMBBqx3Fis0HFS6rEAhKbZSd2cZUXtjOEX4-rlk6IydbV9x3dewA4x-gGI8bmuMhYkiP6MZcVITg9ANP96HD_z_AEnHi_RohySvgxmBCWFRjndAq-723Xd_XYaGdLWHau1bKGtW6DLYdae6hGWNmVDZupXX0G2Luu1N7bdgWV9LqCKhKcjYV2dQslrMZWNpHlB-WDDUPQ0HRujw5Otr6vZRtOwZGRtddnu3cG3h8f3hbPyfL16WVxt0xKkuKQKCVTLg0lOuWU5ylPsVYZM4hoVjFsVCFThTJaGKYQl5WiqGCZNCQGM2oKMgNXW27c_GvQPojG-lLXcQfdDV5EbJ4zkhUoRtE2WrrOe6eNiJc10o0CI7ExLjZKxUav2BqPlYsdfVCNrvaFX8UxcL0NRE1i3Q2ujcf-x7v8I66aRmAuiECEIlyIvjLkBzvQmfY</recordid><startdate>20240501</startdate><enddate>20240501</enddate><creator>Bhutani, Utkarsh</creator><creator>Dey, Namit</creator><creator>Chowdhury, Suvro Kanti</creator><creator>Waghmare, Neha</creator><creator>Mahapatra, Rita Das</creator><creator>Selvakumar, Kamalnath</creator><creator>Chandru, Arun</creator><creator>Bhowmick, Tuhin</creator><creator>Agrawal, Parinita</creator><general>IOP Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6271-9067</orcidid><orcidid>https://orcid.org/0000-0002-1696-7588</orcidid><orcidid>https://orcid.org/0000-0001-9610-8961</orcidid><orcidid>https://orcid.org/0000-0003-2750-9838</orcidid><orcidid>https://orcid.org/0000-0003-2419-114X</orcidid><orcidid>https://orcid.org/0000-0003-0860-4493</orcidid><orcidid>https://orcid.org/0000-0001-6897-4328</orcidid></search><sort><creationdate>20240501</creationdate><title>Biopolymeric corneal lenticules by digital light processing based bioprinting: a dynamic substitute for corneal transplant</title><author>Bhutani, Utkarsh ; Dey, Namit ; Chowdhury, Suvro Kanti ; Waghmare, Neha ; Mahapatra, Rita Das ; Selvakumar, Kamalnath ; Chandru, Arun ; Bhowmick, Tuhin ; Agrawal, Parinita</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c321t-bba29af53e295962921eb48f03e8d81fb7a2b0457f8b09adb50784af392145f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>bioink</topic><topic>Bioprinting</topic><topic>Cornea</topic><topic>cornea lenticule</topic><topic>Corneal Transplantation</topic><topic>digital light processing</topic><topic>gelatin</topic><topic>Humans</topic><topic>hyaluronic acid</topic><topic>Hydrogels</topic><topic>Printing, Three-Dimensional</topic><topic>Tissue Engineering</topic><topic>Tissue Scaffolds - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bhutani, Utkarsh</creatorcontrib><creatorcontrib>Dey, Namit</creatorcontrib><creatorcontrib>Chowdhury, Suvro Kanti</creatorcontrib><creatorcontrib>Waghmare, Neha</creatorcontrib><creatorcontrib>Mahapatra, Rita Das</creatorcontrib><creatorcontrib>Selvakumar, Kamalnath</creatorcontrib><creatorcontrib>Chandru, Arun</creatorcontrib><creatorcontrib>Bhowmick, Tuhin</creatorcontrib><creatorcontrib>Agrawal, Parinita</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical materials (Bristol)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bhutani, Utkarsh</au><au>Dey, Namit</au><au>Chowdhury, Suvro Kanti</au><au>Waghmare, Neha</au><au>Mahapatra, Rita Das</au><au>Selvakumar, Kamalnath</au><au>Chandru, Arun</au><au>Bhowmick, Tuhin</au><au>Agrawal, Parinita</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biopolymeric corneal lenticules by digital light processing based bioprinting: a dynamic substitute for corneal transplant</atitle><jtitle>Biomedical materials (Bristol)</jtitle><stitle>BMM</stitle><addtitle>Biomed. 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The bioprinted lenticules were optically transparent (85.45% ± 0.14%), exhibited adhesive strength (58.67 ± 17.5 kPa), and compressive modulus (535.42 ± 29.05 kPa) sufficient for supporting corneal tissue integration and regeneration. Puncture resistance tests and drag force analysis further confirmed the excellent mechanical performance of the lenticules enabling their application as potential corneal implants. Additionally, the lenticules demonstrated outstanding support for re-epithelialization and stromal regeneration when assessed with human corneal stromal cells. We generated implant ready corneal lenticules while optimizing bioink and bioprinting parameters, providing valuable solution for individuals suffering from various corneal defects and waiting for corneal transplants.</abstract><cop>England</cop><pub>IOP Publishing</pub><pmid>38471165</pmid><doi>10.1088/1748-605X/ad3312</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-6271-9067</orcidid><orcidid>https://orcid.org/0000-0002-1696-7588</orcidid><orcidid>https://orcid.org/0000-0001-9610-8961</orcidid><orcidid>https://orcid.org/0000-0003-2750-9838</orcidid><orcidid>https://orcid.org/0000-0003-2419-114X</orcidid><orcidid>https://orcid.org/0000-0003-0860-4493</orcidid><orcidid>https://orcid.org/0000-0001-6897-4328</orcidid></addata></record> |
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| subjects | bioink Bioprinting Cornea cornea lenticule Corneal Transplantation digital light processing gelatin Humans hyaluronic acid Hydrogels Printing, Three-Dimensional Tissue Engineering Tissue Scaffolds - chemistry |
| title | Biopolymeric corneal lenticules by digital light processing based bioprinting: a dynamic substitute for corneal transplant |
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