Increased Interleukin-4 Production by CD8 and γδ T Cells in Health-Care Workers Is Associated with the Subsequent Development of Active Tuberculosis

We evaluated immune responses to Mycobacterium tuberculosis in 10 health-care workers (HCWs) and 10 non-HCWs and correlated their immune status with the development of active tuberculosis (TB). Twenty individuals were randomly recruited, tested, and monitored longitudinally for TB presentation. Peri...

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Veröffentlicht in:The Journal of infectious diseases 2004-08, Vol.190 (4), p.756-766
Hauptverfasser: Ordway, Diane J., Costa, Leonor, Martins, Marta, Silveira, Henrique, Amaral, Leonard, Arroz, Maria J., Ventura, Fernando A., Dockrell, Hazel M.
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container_end_page 766
container_issue 4
container_start_page 756
container_title The Journal of infectious diseases
container_volume 190
creator Ordway, Diane J.
Costa, Leonor
Martins, Marta
Silveira, Henrique
Amaral, Leonard
Arroz, Maria J.
Ventura, Fernando A.
Dockrell, Hazel M.
description We evaluated immune responses to Mycobacterium tuberculosis in 10 health-care workers (HCWs) and 10 non-HCWs and correlated their immune status with the development of active tuberculosis (TB). Twenty individuals were randomly recruited, tested, and monitored longitudinally for TB presentation. Peripheral blood mononuclear cells (PBMCs) from donors were stimulated with M. tuberculosis and tested for cell proliferation and the production of interferon (IFN)-γ, interleukin (IL)-5, and IL-4, by use of enzyme-linked immunosorbent or flow-cytometric assays. HCWs had higher levels of cell proliferation (24,258 cpm) and IFN-γ (6373 pg/mL) to M. tuberculosis than did non-HCWs (cell proliferation, 11,462 cpm; IFN-γ, 3228 pg/ mL). Six of 10 HCWs showed increased median percentages of CD8+IL-44 (4.7%) and γδ+IL-4 (2.3%) T cells and progressed to active TB. HCWs who remained healthy showed increased median percentages of CDS^FNy+ (25.0%) and γδ+IFN-γ (8.0%) and lower percentages of CD8+-4 (0.05%) and γδ+IL-4 (0.03%) T cells.
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source JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection
subjects Antigens
Bacterial diseases
Biological and medical sciences
Blood
Cell growth
Cytokines
Fundamental and applied biological sciences. Psychology
Human bacterial diseases
Infections
Infectious diseases
Major Articles and Brief Reports
Medical sciences
Microbiology
Mycobacterium tuberculosis
Pulmonary tuberculosis
T lymphocytes
Tuberculin
Tuberculosis
Tuberculosis and atypical mycobacterial infections
title Increased Interleukin-4 Production by CD8 and γδ T Cells in Health-Care Workers Is Associated with the Subsequent Development of Active Tuberculosis
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