Increased Interleukin-4 Production by CD8 and γδ T Cells in Health-Care Workers Is Associated with the Subsequent Development of Active Tuberculosis
We evaluated immune responses to Mycobacterium tuberculosis in 10 health-care workers (HCWs) and 10 non-HCWs and correlated their immune status with the development of active tuberculosis (TB). Twenty individuals were randomly recruited, tested, and monitored longitudinally for TB presentation. Peri...
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Veröffentlicht in: | The Journal of infectious diseases 2004-08, Vol.190 (4), p.756-766 |
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creator | Ordway, Diane J. Costa, Leonor Martins, Marta Silveira, Henrique Amaral, Leonard Arroz, Maria J. Ventura, Fernando A. Dockrell, Hazel M. |
description | We evaluated immune responses to Mycobacterium tuberculosis in 10 health-care workers (HCWs) and 10 non-HCWs and correlated their immune status with the development of active tuberculosis (TB). Twenty individuals were randomly recruited, tested, and monitored longitudinally for TB presentation. Peripheral blood mononuclear cells (PBMCs) from donors were stimulated with M. tuberculosis and tested for cell proliferation and the production of interferon (IFN)-γ, interleukin (IL)-5, and IL-4, by use of enzyme-linked immunosorbent or flow-cytometric assays. HCWs had higher levels of cell proliferation (24,258 cpm) and IFN-γ (6373 pg/mL) to M. tuberculosis than did non-HCWs (cell proliferation, 11,462 cpm; IFN-γ, 3228 pg/ mL). Six of 10 HCWs showed increased median percentages of CD8+IL-44 (4.7%) and γδ+IL-4 (2.3%) T cells and progressed to active TB. HCWs who remained healthy showed increased median percentages of CDS^FNy+ (25.0%) and γδ+IFN-γ (8.0%) and lower percentages of CD8+-4 (0.05%) and γδ+IL-4 (0.03%) T cells. |
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Twenty individuals were randomly recruited, tested, and monitored longitudinally for TB presentation. Peripheral blood mononuclear cells (PBMCs) from donors were stimulated with M. tuberculosis and tested for cell proliferation and the production of interferon (IFN)-γ, interleukin (IL)-5, and IL-4, by use of enzyme-linked immunosorbent or flow-cytometric assays. HCWs had higher levels of cell proliferation (24,258 cpm) and IFN-γ (6373 pg/mL) to M. tuberculosis than did non-HCWs (cell proliferation, 11,462 cpm; IFN-γ, 3228 pg/ mL). Six of 10 HCWs showed increased median percentages of CD8+IL-44 (4.7%) and γδ+IL-4 (2.3%) T cells and progressed to active TB. HCWs who remained healthy showed increased median percentages of CDS^FNy+ (25.0%) and γδ+IFN-γ (8.0%) and lower percentages of CD8+-4 (0.05%) and γδ+IL-4 (0.03%) T cells.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/422532</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: University of Chicago Press</publisher><subject>Antigens ; Bacterial diseases ; Biological and medical sciences ; Blood ; Cell growth ; Cytokines ; Fundamental and applied biological sciences. 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Twenty individuals were randomly recruited, tested, and monitored longitudinally for TB presentation. Peripheral blood mononuclear cells (PBMCs) from donors were stimulated with M. tuberculosis and tested for cell proliferation and the production of interferon (IFN)-γ, interleukin (IL)-5, and IL-4, by use of enzyme-linked immunosorbent or flow-cytometric assays. HCWs had higher levels of cell proliferation (24,258 cpm) and IFN-γ (6373 pg/mL) to M. tuberculosis than did non-HCWs (cell proliferation, 11,462 cpm; IFN-γ, 3228 pg/ mL). Six of 10 HCWs showed increased median percentages of CD8+IL-44 (4.7%) and γδ+IL-4 (2.3%) T cells and progressed to active TB. HCWs who remained healthy showed increased median percentages of CDS^FNy+ (25.0%) and γδ+IFN-γ (8.0%) and lower percentages of CD8+-4 (0.05%) and γδ+IL-4 (0.03%) T cells.</description><subject>Antigens</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Cell growth</subject><subject>Cytokines</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Human bacterial diseases</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Major Articles and Brief Reports</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Mycobacterium tuberculosis</subject><subject>Pulmonary tuberculosis</subject><subject>T lymphocytes</subject><subject>Tuberculin</subject><subject>Tuberculosis</subject><subject>Tuberculosis and atypical mycobacterial infections</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNpFkN1O20AQhVcVSA2hfYNKc9PeGfbHu7YvI6dAJCSQCOIy2l2PFYPjDTt2Kl6EFynPwTPhKKi9OiOdT-doDmPfBT8TPDfnqZRayS9sIrTKEmOEOmITzqVMRF4UX9kJ0SPnPFUmm7DXRecjWsIKFl2PscXhqemSFG5jqAbfN6ED9wLlPAfbVfD-9_0NllBi2xI0HVyhbft1UtqI8BDiE0aCBcGMKPjG9mPqn6ZfQ79GuBsc4fOAXQ9z3GEbtpv9HWqYjTU7hOXgMPqhDdTQKTuubUv47VOn7P7i97K8Sq5vLhfl7DrxUvLxIZlq7Z2X6ArjVCF0YWqpauHq1KHMFHKXycqIWuhKWC1MUfhKcpGpIrW6UlP265DrYyCKWK-2sdnY-LISfLVfc3VYcwR_HsCtJW_bOtrON_SfNmLfnY_cjwP3SH2I_3zFeZYZnasPqRZ_Ag</recordid><startdate>20040815</startdate><enddate>20040815</enddate><creator>Ordway, Diane J.</creator><creator>Costa, Leonor</creator><creator>Martins, Marta</creator><creator>Silveira, Henrique</creator><creator>Amaral, Leonard</creator><creator>Arroz, Maria J.</creator><creator>Ventura, Fernando A.</creator><creator>Dockrell, Hazel M.</creator><general>University of Chicago Press</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20040815</creationdate><title>Increased Interleukin-4 Production by CD8 and γδ T Cells in Health-Care Workers Is Associated with the Subsequent Development of Active Tuberculosis</title><author>Ordway, Diane J. ; Costa, Leonor ; Martins, Marta ; Silveira, Henrique ; Amaral, Leonard ; Arroz, Maria J. ; Ventura, Fernando A. ; Dockrell, Hazel M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2202-12455cbc2eb96b391596f23f1bf4be273e0b72d61f15d1a51699cd2017394a5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Antigens</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Cell growth</topic><topic>Cytokines</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Human bacterial diseases</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Major Articles and Brief Reports</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Mycobacterium tuberculosis</topic><topic>Pulmonary tuberculosis</topic><topic>T lymphocytes</topic><topic>Tuberculin</topic><topic>Tuberculosis</topic><topic>Tuberculosis and atypical mycobacterial infections</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ordway, Diane J.</creatorcontrib><creatorcontrib>Costa, Leonor</creatorcontrib><creatorcontrib>Martins, Marta</creatorcontrib><creatorcontrib>Silveira, Henrique</creatorcontrib><creatorcontrib>Amaral, Leonard</creatorcontrib><creatorcontrib>Arroz, Maria J.</creatorcontrib><creatorcontrib>Ventura, Fernando A.</creatorcontrib><creatorcontrib>Dockrell, Hazel M.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ordway, Diane J.</au><au>Costa, Leonor</au><au>Martins, Marta</au><au>Silveira, Henrique</au><au>Amaral, Leonard</au><au>Arroz, Maria J.</au><au>Ventura, Fernando A.</au><au>Dockrell, Hazel M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased Interleukin-4 Production by CD8 and γδ T Cells in Health-Care Workers Is Associated with the Subsequent Development of Active Tuberculosis</atitle><jtitle>The Journal of infectious diseases</jtitle><date>2004-08-15</date><risdate>2004</risdate><volume>190</volume><issue>4</issue><spage>756</spage><epage>766</epage><pages>756-766</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>We evaluated immune responses to Mycobacterium tuberculosis in 10 health-care workers (HCWs) and 10 non-HCWs and correlated their immune status with the development of active tuberculosis (TB). Twenty individuals were randomly recruited, tested, and monitored longitudinally for TB presentation. Peripheral blood mononuclear cells (PBMCs) from donors were stimulated with M. tuberculosis and tested for cell proliferation and the production of interferon (IFN)-γ, interleukin (IL)-5, and IL-4, by use of enzyme-linked immunosorbent or flow-cytometric assays. HCWs had higher levels of cell proliferation (24,258 cpm) and IFN-γ (6373 pg/mL) to M. tuberculosis than did non-HCWs (cell proliferation, 11,462 cpm; IFN-γ, 3228 pg/ mL). Six of 10 HCWs showed increased median percentages of CD8+IL-44 (4.7%) and γδ+IL-4 (2.3%) T cells and progressed to active TB. HCWs who remained healthy showed increased median percentages of CDS^FNy+ (25.0%) and γδ+IFN-γ (8.0%) and lower percentages of CD8+-4 (0.05%) and γδ+IL-4 (0.03%) T cells.</abstract><cop>Chicago, IL</cop><pub>University of Chicago Press</pub><doi>10.1086/422532</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Antigens Bacterial diseases Biological and medical sciences Blood Cell growth Cytokines Fundamental and applied biological sciences. Psychology Human bacterial diseases Infections Infectious diseases Major Articles and Brief Reports Medical sciences Microbiology Mycobacterium tuberculosis Pulmonary tuberculosis T lymphocytes Tuberculin Tuberculosis Tuberculosis and atypical mycobacterial infections |
title | Increased Interleukin-4 Production by CD8 and γδ T Cells in Health-Care Workers Is Associated with the Subsequent Development of Active Tuberculosis |
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